LOCUS       sequence-422886-        2262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO2a
FEATURES             Location/Qualifiers
     source          1..2262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..582
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     483..502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     699..716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     849..865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     849..865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1188..1205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1188..1204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1204..1275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1276..1344,1345..2136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1494..1513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2137..2156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2137..2155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2244..2262)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
       61 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      121 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      181 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      241 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      361 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      421 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      541 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      601 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      661 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      721 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      781 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatatacac
      841 tccgctagca ggaaacagct atgaccatgc ccgggtaagt gactagcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggctccga agagccgagt
      961 gtcttcggtt cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg
     1021 cgtactagtc tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc
     1081 tgagagaaga cacgtcgctc ttcgacgtaa gtaactgact cggtaccaaa ttccagaaaa
     1141 gaggcctccc gaaagggggg ccttttttcg ttttggtcct aagcttaact ggccgtcgtt
     1201 ttacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2101 gctgagatag gtgcctcact gattaagcat tggtaataat acgactcact atagggtaac
     2161 taattgaggc gcgcctacca ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt
     2221 ttcaaccttc ctaggtaagt gaccctttag tgagggttaa tt
//
