LOCUS       sequence-422895-        2437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  high-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO1te
FEATURES             Location/Qualifiers
     source          1..2437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     630..646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(969..986)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(969..985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1106..2311
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     primer_bind     2312..2331
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2312..2330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2419..2437)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaaggctccg aagagccgag tgtcttcggt tcgagaccga attcgcgagc tctcgctagc
      781 cagtttaaac aattgcggcc gcgtactagt ctatcgattt ggatccaccc tgcaggaagt
      841 ctagaccgtc gacacggtct ctgagagaag acacgtcgct cttcgacgta agtaactgac
      901 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
      961 taagcttaac tggccgtcgt tttacatacg gccccaaggt ccaaacggtg atagggataa
     1021 cagggtaatg taccatttac gttgacacca cctttcgcgt atggcatgac agcgcccgga
     1081 agagagtcaa ttcagggtgg tgaatatgaa tagttcgaca aagatcgcat tggtaattac
     1141 gttgctcgat gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga
     1201 atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt
     1261 aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc
     1321 agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag
     1381 tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc
     1441 tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg
     1501 ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc
     1561 aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcacttt
     1621 ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga
     1681 agtaggggtt gagacgcaat caaattcggt gtacatcact ttatttaaaa cgatgcccat
     1741 tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt
     1801 gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg
     1861 tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg
     1921 gggcgaaaaa acggcagtac tgctcggatt tattgcagat agtagtgcat ttgccttttt
     1981 agcgtttata tctgaaggtt ggttagtttt ccctgtttta attttattgg ctggtggtgg
     2041 gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc atcagcaagg
     2101 tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg gcccattact
     2161 gtttgctgtt atttataatc attcactacc aatttgggat ggctggattt ggattattgg
     2221 tttagcgttt tactgtatta ttatcctgct atcaatgacc ttcatgttga cccctcaagc
     2281 tcaggggagt aaacaggaga caagtgctta gtaatacgac tcactatagg gtaactaatt
     2341 gaggcgcgcc taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
     2401 ccttcctagg taagtgaccc tttagtgagg gttaatt
//
