LOCUS       sequence-42291-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MPP8 (3R93)
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             300..317
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(370..388)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      384..431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(555..574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcgag gatgtgttcg aggtggagaa gatcctggac
      121 atgaagaccg aggggggtaa agttctttac aaagttcgct ggaaaggcta tacatcggat
      181 gatgatacct gggagcccga gattcacctg gaggactgta aagaagtgct tcttgaattt
      241 aggaagaaaa ttgcagagaa caaagccaaa tgatgacgaa gcttgcggcc gcactcgagc
      301 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      361 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      421 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      481 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      541 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      601 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      661 cctcgac
//
