LOCUS       sequence-42292-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00413
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(634..733)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(749..766)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(749..765)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
       61 ttataatgcc aactttgtac aagaaagttg gcaaccaggg gcgccacaag ggctgctgca
      121 tgggctgtgg cgagggggcg gggctgtcgc agtcgctgtg atatccggag gaggcggggc
      181 tgagcggagc cttgtccatt gaggagcagt ccatcgagtt ctggatgaag tcggcggcgg
      241 actgtgcttc gtggaattgc tgcaagttct tgtagacgcg tcccaggtga gtcatcaccg
      301 atttgcccag gtcgacgctc atgccaggtg tggaggccat gacacgtgac acctcgttga
      361 cggcattcat gtagccattc ttaaagctgt ccagggggag ggattgttct tcctgagcca
      421 ccttctttgg tgtcttttgc tggcgcatga agatcaccgc tgactcgagc atctcggcct
      481 tgtccatgcg caggatgcca tcatcacctc gtagctcggc gacaagtgtt ttcaggttgt
      541 ccaggcactt gttcatgcgg gcacgtcgct ggcgctccag cattggcttc ttcaccttct
      601 ggtagatctg ggtcttggtg gtgtattcca tggtgccaac ttttttgtac aaagttggca
      661 ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      721 aaatcattat ttggggcccg agcttaagac tggccgtcgt tttacaacgt
//
