LOCUS       sequence-422934-        5790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses Nanoluciferase (Nluc) containing the 
            Argonaut (AGO) intron (405 bp into the gene) with the truncated 
            35sCAMV promoter and the tomato mosaic virus UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35s[TMV]:NLuc-I{AGO-405}:NosT
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     658..678
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        667..713
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1424..1676
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1852..1871)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1863..2678
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1863..2678
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1936..1955)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2764..3352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3253..3272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3594..4223
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4652..5725
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcgga aacctcctcg gattccattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
      721 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
      781 aattacattt acaattatcg atacacgcgt atggtgttta ctcttgaaga tttcgtggga
      841 gactggcgtc aaacagcagg ctataactta gaccaggtcc tagaacaggg aggcgtttcc
      901 agtctgtttc agaatcttgg agttagtgtt acacctatcc aaagaatagt tctcagcggt
      961 gaaaatggcc tcaaaataga catccatgtg atcatcccat acgagggctt gtccggcgat
     1021 caaatgggcc agatagagaa aatattcaaa gtagtgtacc cagtagatga ccaccacttt
     1081 aaggtaatac ttcactacgg gaccttagtt atagacgggg taacccctaa tatgatcgac
     1141 tacttcggcc gtccctacga gggcattgct gtcttcgatg ggaagaagat aacagttacc
     1201 ggcactttgt ggaacggtaa gtttcatcta tcaactcaaa actttatttg ttaatgtttg
     1261 ctcctctgaa tcgttttaat actctttatg ttctgaatta ggtaacaaaa tcatcgacga
     1321 gcgactgatc aacccagatg gtagccttct gttcagggtt accattaacg gggttacagg
     1381 gtggcgtctg tgcgagcgta tattagctta agtatacctg caggatcgtt caaacatttg
     1441 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     1501 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     1561 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     1621 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggg
     1681 agctctggca ggatatatgc ggttgtaatt catttttatt gtctaaattt ctgtatttgt
     1741 ttgtttgcaa ttagatccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     1801 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1861 ttatgagcca tattcaacgg gaaacgtctt gttcgaggcc gcgattaaat tccaacatgg
     1921 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1981 tctaccgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2041 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2101 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2161 cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2221 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2281 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2341 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2401 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2461 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2521 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2581 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2641 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2701 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa tcaattgatc
     2761 ttcttgaaat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2881 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2941 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3061 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3121 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3181 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3241 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3361 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3421 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgat ctctgaagaa
     3481 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     3541 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     3601 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     3661 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     3721 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     3781 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     3841 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     3901 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     3961 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     4021 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     4081 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     4141 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     4201 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     4261 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     4321 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     4381 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     4441 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     4501 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     4561 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     4621 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     4681 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     4741 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     4801 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     4861 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     4921 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     4981 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     5041 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     5101 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     5161 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     5221 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     5281 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     5341 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagttg attggatgta
     5401 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     5461 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     5521 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     5581 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     5641 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     5701 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     5761 aggggaaaaa ggtcgaaaag atctctttcc
//
