LOCUS       sequence-42294-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TDRD7 (3RCO)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             530..547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(600..618)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      614..661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcatgctgg aaggagatct ggtttcaaag atgctacgag
      121 ctgttctgca gtctcataag aatggagtag cattaccccg gctccaagga gagtacagat
      181 ccttgactgg agactggatc cccttcaaac agctaggttt ccctacacta gaagcctatc
      241 tgagaagtgt gccagcagtg gtcaggatag agactagtag atctggagag attacctgct
      301 atgccatggc ctgcacagaa actgcaagaa ttgctcagct tgtggctcgt caaaggagtt
      361 ctaaaaggaa aaccgggcgt caagttaatt gtcagatgag agtgaagaaa accatgccat
      421 tttttctaga aggaaaacca aaagcaaccc tcagacaacc aggatttgct tcaaattttt
      481 ctgttggcaa aaaacctaat tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      541 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      601 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      661 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      781 cccgctcctt tcgc
//
