LOCUS       sequence-422971-        5451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector with N terminal HA  tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5451)
  TITLE     Emory Custom Cloning Core Plasmids - Oskar Laur
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-HA
FEATURES             Location/Qualifiers
     source          1..5451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     560..579
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(798..902)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(977..996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1168..1547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1548..1751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1702..1722
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1796..1815
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1796..1814
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1843..1869
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1975..1992)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1981..2205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2251..2679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2338..2357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2548..2569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2688..2708)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2693..3022
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2873..3008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2935..2954
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3089..3883
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3143..3162)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3753..3772
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4057..4178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4094..4113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4148..4167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4227..4243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4227..4243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4240..4262)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4251..4267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4275..4281,4282..4299,4300..4305))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4320..4341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4458..4475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4629..5217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4709..4728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5388..5451,1..728,729..797))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
       61 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      121 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      181 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      241 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      301 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      361 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      421 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      481 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      541 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      601 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      661 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      721 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      781 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      841 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      901 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
      961 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     1021 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     1081 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     1141 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     1201 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     1261 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1321 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1381 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1441 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1501 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1561 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1621 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1681 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1741 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     1801 cgactcacta tagggagacc caagctggct agcgccatgg tttacccata cgatgttcca
     1861 gattacgctg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
     1921 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     1981 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2041 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2101 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2161 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     2221 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     2281 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2341 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2401 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2461 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2521 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2581 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2641 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     2701 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2761 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2821 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2881 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2941 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     3001 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     3061 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3121 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3181 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3241 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3301 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3361 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3421 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3481 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3541 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3601 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3661 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3721 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3781 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3841 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     3901 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     3961 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     4021 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     4081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     4201 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4261 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4321 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4381 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4441 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4501 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4561 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4621 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4681 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4741 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4801 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4861 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4921 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4981 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5041 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5101 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5161 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5221 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5281 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5341 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5401 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt t
//
