LOCUS       sequence-422977-        6011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mushroom luciferase (nnLuz) with the 
            truncated 35sCAMV promoter and Tomato mosaic virus UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6011)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35s[TMV]:nnLuz:NosT
FEATURES             Location/Qualifiers
     source          1..6011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             723..1796
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1862..2056
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     2519..2539
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        2528..2574
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             complement(3148..3159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      3506..3758
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3934..3953)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3945..4760
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3945..4760
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4018..4037)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4846..5434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5335..5354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(5676..6011,1..294)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
       61 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
      121 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
      181 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
      241 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
      301 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
      361 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
      421 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
      481 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
      541 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
      601 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
      661 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
      721 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
      781 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
      841 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
      901 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
      961 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     1021 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     1081 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     1141 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     1201 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     1261 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     1321 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     1381 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     1441 acatcgagat cgagctagtt gattggatgt accgcgagat cacagaaggc aagaacccgg
     1501 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     1561 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     1621 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     1681 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     1741 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     1801 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa gatctctttc
     1861 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     1921 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     1981 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     2041 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     2101 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     2161 gccgctcaaa aatggctggc ctacggccag gcaatctgct agcgcgcgga caagctagga
     2221 ttggcaggat atattcagtt gtaaatgata tcgcatgcgg aaacctcctc ggattccatt
     2281 gcccagctat ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc tcctacaaat
     2341 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     2401 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     2461 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     2521 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct
     2581 cgagtataag agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     2641 caattacatt tacaattatc gatacatcga gggtaccatg aggataaata tctctttgtc
     2701 tagtctcttt gagagactga gcaaattgtc atcccgatcc atcgcaatta cctgcggcgt
     2761 agtcttggca agtgcaatag cattcccaat tatccgaaga gactatcaaa cgttccttga
     2821 agtcggtccg agctatgccc cacagaactt ccgaggctat atcatcgttt gtgtcttgtc
     2881 actttttagg caagagcaga aagggttggc aatctatgac aggcttccag aaaagaggcg
     2941 ttggcttgcc gacttgccgt ttcgtgaagg gacgaggcca tccataacct cccacattat
     3001 acagcgtcag cgtactcagc tggtagatca agagtttgca accagagaac ttatcgataa
     3061 ggtgatccca cgtgtgcaag caagacacac agacaaaact tttctcagta cgtcaaaatt
     3121 tgagtttcat gcaaaggcca tcttccttct cccctctatc cctattaatg atcctcttaa
     3181 cattccctca cacgatacgg taagaagaac caagcgtgag atcgctcaca tgcatgatta
     3241 ccatgattgc actctgcact tggctcttgc tgctcaggat ggtaaggaag ttttgaagaa
     3301 ggggtggggc cagcgtcacc cactggccgg accaggagtc ccaggccctc ctactgagtg
     3361 gaccttcctt tacgcaccaa ggaacgaaga ggaggccaga gttgtcgaga tgatagtcga
     3421 agctagtatt ggctacatga ctaacgatcc tgctggtaag attgtcgaaa atgccaagtc
     3481 aggttgagaa ttcggatccc tgcaggatcg ttcaaacatt tggcaataaa gtttcttaag
     3541 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     3601 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     3661 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     3721 taaattatcg cgcgcggtgt catctatgtt actagatcgg ggagctctgg caggatatat
     3781 gcggttgtaa ttcattttta ttgtctaaat ttctgtattt gtttgtttgc aattagatcc
     3841 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     3901 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     3961 gggaaacgtc ttgttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     4021 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctaccga ttgtatggga
     4081 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     4141 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     4201 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag
     4261 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     4321 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     4381 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     4441 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     4501 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     4561 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     4621 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     4681 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     4741 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     4801 cgctgacttg acgggacggc ggctttgttg aatcaattga tcttcttgaa atcctttttt
     4861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4981 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5521 tgtggataac cgtattaccg cctttgagtg atctctgaag aaaccgagcg ccgccgtcta
     5581 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     5641 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     5701 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     5761 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     5821 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     5881 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     5941 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     6001 cttacgacat a
//
