LOCUS       sequence-422981-        9076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens. This is the control vector 
            for the collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3550
FEATURES             Location/Qualifiers
     source          1..9076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        372..476
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(477..545,546..1337)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(695..714)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1508..2096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1997..2016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2250..2267
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2384..2405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2420..2425,2426..2443,2444..2450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2458..2474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2463..2485
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2482..2498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2482..2498
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2517..2537
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2519..2537
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2565..2791
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2792..2972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3019..3144
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3637..3870
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4055..4099
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4330..4540
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4352..4548
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4399..4534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4580..5182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(4580..4599)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5076..5096
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5225..5342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5480..6658
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5711..6649
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6606..6626
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6669..6678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6675..7385
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6820..6838)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7088..7107
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7493..8081
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7546..7566)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7964..7975)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8153..8386
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8464..8585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8501..8520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8555..8574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8625..8760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8780..8799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8781..8799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8809..8826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8809..8825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8818..8840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8967..9076,1..346)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9054..9073)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
       61 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      301 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
      361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      661 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      781 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      841 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      901 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      961 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1021 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1081 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1141 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1201 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1261 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1321 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1381 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2161 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2221 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2281 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2341 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2401 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2461 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     2521 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     2581 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     2641 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     2701 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     2761 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     2821 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2881 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2941 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     3001 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     3061 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     3121 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     3181 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     3241 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     3301 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     3361 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     3421 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     3481 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     3541 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     3601 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     3661 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     3721 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     3781 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     3841 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     3901 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3961 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     4021 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     4081 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     4141 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     4201 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     4261 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4321 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     4381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     4561 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4621 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4681 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4741 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     4801 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4861 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4921 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4981 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5041 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5101 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5161 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     5221 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     5281 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     5341 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     5401 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     5461 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     5521 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     5581 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     5641 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     5701 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     5761 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     5821 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     5881 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     5941 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     6001 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     6061 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     6121 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     6181 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     6241 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     6301 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     6361 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     6421 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     6481 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     6541 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     6601 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     6661 ctagcaccgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     6721 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     6781 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     6841 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     6901 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     6961 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     7021 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     7081 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     7141 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     7201 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     7261 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     7321 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     7381 agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     7441 gtggaaagga cgaagagaat atcacgtccg tgcaaagacg cgttaagtcg acaatcaacc
     7501 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7561 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7621 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7681 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7741 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7801 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7861 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7921 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7981 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8041 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     8101 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8161 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     8221 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8281 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8341 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     8401 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8461 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8521 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8581 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     8641 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8701 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8761 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8821 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8881 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8941 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     9001 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9061 cgctttcttc ccttcc
//
