LOCUS       sequence-422983-        9365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a codon-optimized ORF of human SF3B1 (wild-type).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Kesarwani AK, Ramirez O, Gupta AK, Yang X, Murthy T, Minella AC, 
            Pillai MM
  TITLE     Cancer-associated SF3B1 mutants recognize otherwise inaccessible 
            cryptic 3' splice sites within RNA secondary structures.
  JOURNAL   Oncogene. 2016 Aug 15. doi: 10.1038/onc.2016.279.
  PUBMED    27524419
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2016 Aug 15. doi: 10.1038/onc.2016.279."
COMMENT     Sequence Label: pCDNA3.1-FLAG-SF3B1-WT
FEATURES             Location/Qualifiers
     source          1..9365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..238
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     189..209
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     283..302
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        283..301
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             361..384
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4376..4393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4382..4606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4652..5080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4739..4758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4949..4970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5089..5109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5094..5423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5274..5409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5336..5355
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5490..6284
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5544..5563)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6154..6173
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6458..6579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6495..6514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6549..6568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6628..6644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6628..6644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6641..6663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6652..6668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6676..6682,6683..6700,6701..6706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6721..6742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6859..6876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7030..7618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7110..7129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7789..8580,8581..8649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8412..8431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8650..8754)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8829..8848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9020..9365,1..34)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
       61 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      121 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      181 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
      241 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
      301 ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg atccaccatg
      361 gactacaaag atgacgacga caagtctaga atggctaaga tcgctaaaac ccacgaggac
      421 attgaggcac agattcggga gattcagggc aagaaggcag cactggacga ggcacagggc
      481 gtggggctgg atagcaccgg ctactatgac caggaaatct acggcgggtc tgacagtaga
      541 ttcgccggct acgtgaccag catcgccgct accgagctgg aagacgatga cgatgactat
      601 agctcctcta cttcactgct ggggcagaag aaacctggat accacgcacc agtggccctg
      661 ctgaacgata tcccacagtc caccgagcag tatgacccct tcgctgaaca ccggccccct
      721 aagattgcag atagagagga cgaatacaag aaacatcgga gaacaatgat catttctcca
      781 gagcggctgg accccttcgc cgacggaggc aaaacacctg atccaaagat gaacgccagg
      841 acttacatgg acgtgatgcg cgaacagcat ctgaccaagg aggaacggga gatcagacag
      901 cagctggccg agaaggcaaa agccggagaa ctgaaagtcg tcaatggcgc agccgcttct
      961 cagccaccca gtaagaggaa aaggcgctgg gatcagaccg ctgaccagac acctggagca
     1021 actccaaaga aactgagttc atgggatcag gcagagaccc caggacacac acctagcctg
     1081 cgatgggacg aaacccctgg acgagcaaag ggctccgaga ctccaggagc taccccaggc
     1141 agcaagattt gggaccccac accttctcat actccagctg gcgcagcaac cccaggaaga
     1201 ggcgacacac caggacacgc aactccagga catggaggag ctacaagctc cgcaaggaaa
     1261 aatcgctggg atgagacccc taagacagaa agggacactc cagggcacgg atcaggctgg
     1321 gccgaaactc cacgaaccga tcgaggaggg gacagcatcg gggagactcc aacccccgga
     1381 gcttcaaaga gaaaaagcag atgggatgag acacccgcat cccagatggg aggctctacc
     1441 cctgtgctga caccaggaaa gactcccatc ggcacccctg ccatgaacat ggctacacct
     1501 actccaggcc atattatgtc tatgacccca gaacagctcc aggcctggcg atgggagcga
     1561 gaaatcgacg agcgcaatcg acctctgagt gatgaggaac tggacgctat gttcccagag
     1621 ggctataagg tgctgcctcc acctgccggc tacgtcccaa tcagaactcc cgccaggaaa
     1681 ctgaccgcta cacccactcc tctgggggga atgaccgggt ttcacatgca gacagaggat
     1741 cgcactatga agagcgtgaa cgaccagcct tccggaaatc tgcctttcct gaagccagat
     1801 gacatccagt actttgataa gctgctggtg gacgtggacg aatccacact gtctccagag
     1861 gaacagaagg agcgcaaaat catgaagctg ctgctgaaga tcaagaacgg cacacctcca
     1921 atgcgaaagg ctgcactgcg gcagatcact gacaaagcac gggagttcgg ggccggaccc
     1981 ctgtttaatc agattctgcc tctgctgatg tccccaaccc tggaggatca ggaacggcac
     2041 ctgctggtga aagtcatcga cagaatcctg tataagctgg atgacctggt gcgcccctac
     2101 gtccataaga tcctggtggt cattgagcct ctgctgatcg atgaagacta ctatgcccgc
     2161 gtggaggggc gagaaatcat ttctaacctg gccaaggccg ctggactggc taccatgatc
     2221 agtacaatgc ggccagatat tgacaacatg gacgagtatg tgagaaatac cacagctagg
     2281 gcattcgccg tggtcgcttc tgcactgggc atccccagtc tgctgccttt tctgaaagcc
     2341 gtgtgcaaga gtaagaaatc atggcaggca cggcacacag gcatcaagat tgtgcagcag
     2401 atcgcaattc tgatggggtg tgccatcctg ccccacctga gaagcctggt ggagatcatt
     2461 gaacatggcc tggtggatga gcagcagaag gtcaggacca tctccgccct ggctattgca
     2521 gccctggcag aggctgcaac accatacggg atcgagagct tcgactccgt gctgaaaccc
     2581 ctgtggaagg gcatccggca gcacagaggc aaaggactgg cagcttttct gaaggccatc
     2641 ggctatctga ttcccctgat ggatgctgag tacgcaaact actatacccg cgaagtgatg
     2701 ctgatcctga ttcgagagtt ccagagccct gatgaggaaa tgaagaaaat cgtcctgaaa
     2761 gtggtcaagc agtgctgtgg caccgacgga gtggaggcca attatatcaa gacagaaatt
     2821 ctgccccctt tctttaaaca cttttggcag cataggatgg ctctggatcg acggaactac
     2881 cgccagctgg tggacactac cgtcgaactg gccaataagg tcggagcagc cgagatcatt
     2941 agccgcatcg tcgatgacct gaaagacgag gccgaacagt accggaagat ggtcatggag
     3001 accatcgaaa aaattatggg aaacctgggc gccgccgaca ttgaccacaa gctggaggaa
     3061 cagctgatcg atggcattct gtatgccttc caggagcaga caactgaaga cagcgtgatg
     3121 ctgaacggat ttggcaccgt ggtcaatgcc ctgggaaaaa gagtgaagcc atacctgccc
     3181 cagatttgcg gcacagtcct gtggcggctg aacaacaaga gtgctaaggt gagacagcag
     3241 gccgctgatc tgatttcaag gactgccgtg gtcatgaaaa cctgtcagga ggaaaagctg
     3301 atggggcacc tgggagtggt cctgtacgaa tatctgggcg aggaataccc tgaggtgctg
     3361 ggctccatcc tgggggccct gaaggctatc gtgaacgtca ttgggatgca caaaatgact
     3421 ccacccatca aggacctgct gccaaggctg acccccattc tgaaaaaccg ccatgagaag
     3481 gtgcaggaaa attgcatcga tctggtcggc cggattgcag acagaggagc agagtacgtg
     3541 agcgcccggg agtggatgcg catctgtttc gagctgctgg aactgctgaa ggctcacaag
     3601 aaagcaatta gaagggccac agtgaatact tttgggtaca tcgcaaaggc cattggacca
     3661 catgacgtgc tggccaccct gctgaacaat ctgaaagtcc aggagaggca gaaccgcgtg
     3721 tgcaccacag tcgccatcgc catcgtggcc gagacctgtt cccccttcac agtcctgcct
     3781 gctctgatga atgagtatag ggtgcccgaa ctgaacgtgc agaatggggt cctgaagtca
     3841 ctgagcttcc tgtttgagta catcggcgaa atggggaaag attacatcta cgcagtgaca
     3901 cctctgctgg aggacgccct gatggatcgc gacctggtgc accgacagac tgcctcagct
     3961 gtggtccagc atatgagcct gggcgtgtat gggttcggat gcgaggactc cctgaaccac
     4021 ctgctgaatt acgtgtggcc caacgtcttt gaaacctctc ctcatgtgat ccaggctgtc
     4081 atgggagcac tggagggcct gagagtggcc attggacctt gcaggatgct ccagtattgt
     4141 ctccagggcc tgtttcaccc agcccgaaaa gtgcgggatg tctactggaa aatctacaac
     4201 agtatctaca tcggctcaca ggacgcactg attgcccatt accccagaat ctacaatgac
     4261 gacaaaaata cttacatccg atacgaactg gactacatcc tgtgagaatt ctgcagatat
     4321 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     4381 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4441 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4501 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4561 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4621 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4861 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4981 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5101 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5161 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5221 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5281 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5341 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5401 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5461 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5521 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5581 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5641 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5701 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5761 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5821 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5881 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5941 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6001 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6061 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6121 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6181 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6241 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6301 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6361 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6421 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6601 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8101 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8401 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8761 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8821 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8881 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     8941 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     9001 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     9061 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9121 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9181 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9241 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9301 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9361 tggca
//
