LOCUS       sequence-42300-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MLH1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctgaatatt ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gctcccgaaa ggaaatgact gcagcttgta
      121 ccccccggag aaggatcatt aacctcacta gtgttttgag tctccaggaa gaaattaatg
      181 agcagggaca tgaggttctc cgggagatgt tgcataacca ctccttcgtg ggctgtgtga
      241 atcctcagtg ggccttggca cagcatcaaa ccaagttata ccttctcaac accaccaagc
      301 ttagtgaaga actgttctac cagatactca tttatgattt tgccaatttt ggtgttctca
      361 ggttatcgga gccagcaccg ctctttgacc ttgccatgct tgccttagat agtccagaga
      421 gtggctggac agaggaagat ggtcccaaag aaggacttgc tgaatacatt gttgagtttc
      481 tgaagaagaa ggctgagatg cttgcagact atttctcttt ggaaattgat gaggaaggga
      541 acctgattgg attacccctt ctgattgaca actatgtgcc ccctttggag ggactgccta
      601 tcttcattct tcgactagcc actgaggtga attgggacga agaaaaggaa tgttttgaaa
      661 gcctcagtaa agaatgcgct atgttctatt ccatccggaa gcagtacata tctgaggagt
      721 cgaccctctc aggccagcag agtgaagtgc ctggctccat tccaaactcc tggaagtgga
      781 ctgtggaaca cattgtctat aaagccttgc gctcacaca
//
