LOCUS       sequence-423000-        9562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoted WBeta recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: pJT87
FEATURES             Location/Qualifiers
     source          1..9562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1060..1896
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    2069..2090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2105..2110,2111..2128,2129..2135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2143..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2167..2183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2526..2545
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2544..2599
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2645..2664
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    2754..2778
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4236..4260)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4291..4310)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4318..5337
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4372..4394)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5338..5612)
                     /label=chicken beta-actin promoter
     enhancer        5614..5917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(6027..6045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6052..6068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6150..6170)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6838..6863
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    6942..7745
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(7760..7778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7788..7804)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7946..8401
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8033..8052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8243..8264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8427..8531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8532..8600,8601..9392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8750..8769)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtgga gagcagagcc aggaacccct
     1081 gtagggaagg ggcaggagag ccaggggcat gagatggtgg acgaggaagg gggacaggga
     1141 agcctgagcg cctctcctgg gcttgccaag gactcaaacc cagaagccca gagcagggcc
     1201 ttagggaagc gggaccctgc tctgggcgga ggaatatgtc ccagatagca ctggggactc
     1261 tttaaggaaa gaaggatgga gaaagagaaa gggagtagag gcggccacga cctggtgaac
     1321 acctaggacg caccattctc acaaagggag ttttccacac ggacaccccc ctcctcacca
     1381 cagccctgcc aggacggggc tggctactgg ccttatctca caggtaaaac tgacgcacgg
     1441 aggaacaata taaattgggg actagaaagg tgaagagcca aagttagaac tcaggaccaa
     1501 cttattctga ttttgttttt ccaaactgct tctcctcttg ggaagtgtaa ggaagctgca
     1561 gcaccaggat cagtgaaacg caccagacag ccgcgtcaga gcagctcagg ttctgggaga
     1621 gggtagcgca gggtggccac tgagaaccgg gcaggtcacg catccccccc ttccctccca
     1681 ccccctgcca agctctccct cccaggatcc tctctggctc catcgtaagc aaaccttaga
     1741 ggttctggca aggagagaga tggctccagg aaatgggggt gtgtcaccag ataaggaatc
     1801 tgcctaacag gaggtggggg ttagacccaa tatcaggaga ctaggaagga ggaggcctaa
     1861 ggatggggct tttctgtcac caatcctgtc cctagtagcg gccgcgtcga cgtttaaacc
     1921 tcgagattac cctgttatcc ctattaatta aggatcttaa aaacattata caatactaca
     1981 agcataaaaa cgcaccaagc ttagtactgt cgatccgctg gcacgacagg tttcccgact
     2041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     2101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     2161 ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat
     2221 ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
     2281 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
     2341 aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca
     2401 gggggctgtt tcatatactg atgacctctt tatagccaac ctttgttcat ggcagccagc
     2461 atatgggcat atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt
     2521 gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg
     2581 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
     2641 taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca
     2701 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcacg cgtaccactt
     2761 tgtacaagaa agctgggtat cacaggctga aggtgtattc gatgtcaatg tatttgtctt
     2821 tgctcagggt aattttcttg atcagagtct tgaacacttc atgcatatct ttttcctcgt
     2881 ccagcattga aaaagcctcc ttaattttct tttcctcctt gtttcgcttg ttctggtctg
     2941 tcagcttcag cagggccagt tcctgctctt tgatttcatt ctcgaagttc aggtcgcgct
     3001 ttgaaaacat ttctttatca atcaggccct cgacgtacag gtcgatcagc ttttctttct
     3061 tcactttcag cagcttaatc tgtttcttga tctcggtact cttgtcattc cgtttctgat
     3121 gcatctttgg gttaaagtgg atttccagct ctttttcctg ctgcttcagt ccgtagatga
     3181 tggcctcgcg aatatcgtcg tagtgtgcag gatcgaaatt cacgcagcgg ttaaacctga
     3241 tataattgga gcacttcatg tacacccact ctttcttgga ccccttagca tacctgccgc
     3301 tgtatttggc agtgataggt tctccacaat gcttgcagaa catcatcccc cgaaactcgt
     3361 ttcgagtgtc ctgttttgtt ttcttttttg atgcgcgatc actattgaca gcgttccaca
     3421 gctccttact cacgattgta ggatagtggt cttcaatgat tgtcagcctc tccttggggg
     3481 tgtacactct ctttttcttg ccatcgactt tcacagtctt ataactattc aggaccaggt
     3541 tccccttgta tgtctgattt ttcaggatct tgcccactgt ggtatgagac cacagctggc
     3601 cgaacttggt ataagttcct ttgtcattca gaatgtttgc gattttcttg aacccatagc
     3661 ccttgtagct cagatcaaaa atctctctca cgatctcagc ttctttctcg ttaatgacca
     3721 gtttcttatc gatcacgtcg tatcccagcc cgggctttcc gataaactcc ccgcgccttg
     3781 ctttagcctg catggctgca gagacggaca cgctaatggt ctttggcagc tgagcggcga
     3841 acatggcaaa catttcgaat ttgatatcat tcccgccctc atacagagag tcgtagtttt
     3901 cctcaatagt caccagccgg atgccatgtc caatcaggat ttccttaatc tccagggcat
     3961 ctctcaggtc ccgtgccagt cggtggatag acttgaagac cactgtatcc agttcatttc
     4021 tccgggcttt ctccagaatc agctgcatag cgtgcctctc cagccatgcg gttccggaga
     4081 ttccatcgtc aaagtacacg gcattggggt cccattcata cccgttcttc tccagccagt
     4141 atctgcaaat atcgatctgg ttttcgacgg aagagacctg ctcatcccta tctgtgctga
     4201 ctctgacgta gactgcgtat ttcatggtgg caggcctcct gcttttttgt acaaacttgg
     4261 gtcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg
     4321 taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg
     4381 ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca
     4441 gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc
     4501 gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg
     4561 gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct
     4621 ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc
     4681 gctccggggg ccgccgcgcc cctcccccga gccctccccg gcccgaggcg gccccgcccc
     4741 gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg cgccacgccc
     4801 cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag gggggtgcag
     4861 ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca cgcacacacc
     4921 ccgcccgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac cgcccccggc
     4981 cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc
     5041 acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac acacgcacgc
     5101 accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa ggctttcacg
     5161 cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc ccggaggaga
     5221 agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg
     5281 gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcaa cgcagcgact cgactcccgc
     5341 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     5401 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg
     5461 cccctcgccc cgccccgccc cgcctggcgc gcgccccccc ccccccgccc ccatcgctgc
     5521 acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg
     5581 gagagtgaag cagaacgtgg ggctcacctc gaccatggta atagcgatga ctaatacgta
     5641 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     5701 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     5761 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     5821 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     5881 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     5941 aactaatgac cccgtaattg attactatta ataactagtc caccgcggtg gagctcgcgc
     6001 gctacgtagg cctagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt
     6061 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6121 acatttaatt aactcgagaa attcggagcc aacttttcta tacaaagttg atatcactag
     6181 cgttaacgtc gacatcgata cggtaccgag ttggcgcgcc tgggagctca cggggacagc
     6241 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     6301 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     6361 ggggacagcc cgcggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
     6421 ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat
     6481 ttagaatgac aggcgcgcct gggagctcac ggggacagcc ccctcccaaa gcccccaggg
     6541 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     6601 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     6661 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     6721 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacaggcgcg
     6781 ccaactcgaa ttcattgcag actttcactg gtaccatcga tgcaattgtc tcgaggccct
     6841 gtgggaggaa gagaagaggt cagaagcttg gcacagtcaa tgaagtttga aaaccaataa
     6901 gagctcggta aaccggttag ggataacagg gtaataagct tgccccactg tggggtggag
     6961 gggacagata aaagtaccca gaaccagagc cacattaacc ggccctggga atataaggtg
     7021 gtcccagctc ggggacacag gatccctgga ggcagcaaac atgctgtcct gaagtggaca
     7081 taggggcccg ggttggagga agaagactag ctgagctctc ggacccctgg aagatgccat
     7141 gacagggggc tggaagagct agcacagact agagaggtaa ggggggtagg ggagctgccc
     7201 aaatgaaagg agtgagaggt gacccgaatc cacaggagaa cggggtgtcc aggcaaagaa
     7261 agcaagagga tggagaggtg gctaaagcca gggagacggg gtactttggg gttgtccaga
     7321 aaaacggtga tgatgcaggc ctacaagaag gggaggcggg acgcaaggga gacatccgtc
     7381 ggagaaggcc atcctaagaa acgagagatg gcacaggccc cagaaggaga aggaaaaggg
     7441 aacccagcga gtgaagacgg catggggttg ggtgagggag gagagatgcc cggagaggac
     7501 ccagacacgg ggaggatccg ctcagaggac atcacgtggt gcagcgccga gaaggaagtg
     7561 ctccggaaag agcatccttg ggcagcaaca cagcagagag caaggggaag agggagtgga
     7621 ggaagacgga acctgaagga ggcggcaggg aaggatctgg gccagccgta gaggtgaccc
     7681 aggccacaag ctgcagacag aaagcggcac aggcccaggg gagagaatgc tggtcagaga
     7741 aagcaggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     7801 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7861 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7921 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7981 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8041 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8101 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8161 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8221 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8281 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8341 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8401 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8461 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8521 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8581 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8641 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8701 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8761 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8821 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8881 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8941 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9001 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9061 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9121 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9181 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9241 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9301 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9361 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9421 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9481 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9541 tagaaaagat caaaggatct tc
//
