LOCUS       sequence-423003-        6143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse talin1 head (1-490)-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Rahikainen R, Ohman T, Turkki P, Varjosalo M, Hytonen VP
  TITLE     Talin-mediated force transmission and talin rod domain unfolding 
            independently regulate adhesion signaling.
  JOURNAL   J Cell Sci. 2019 Apr 3;132(7):jcs226514. doi: 10.1242/jcs.226514.
  PUBMED    30837291
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-3"; volume: "132(7):jcs226514. doi"; pages: " 
            10.1242/jcs.226514"
COMMENT     Sequence Label: Talin1 head-mCherry
FEATURES             Location/Qualifiers
     source          1..6143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2080..2790
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2225..2243)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2493..2512
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2931..3052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2968..2987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3022..3041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3059..3514)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3196..3217)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3408..3427
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3541..3645
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3647..4004
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3647..3838
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3670..3690)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3855..3990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4039..4833
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4039..4833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4093..4112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4703..4722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5021..5040)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5065..5112
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5441..6029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5930..5949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcacca tggttgcgct ttcgctgaag attagcattg ggaatgtggt gaagacgatg
      661 caatttgagc catctaccat ggtgtacgac gcctgccgca tgattcgtga gcggatccca
      721 gaggccctgg ctggccctcc caacgacttt gggctctttc tgtcagatga tgaccccaaa
      781 aaaggcatct ggctagaggc tgggaaagct ttggactact acatgctccg aaatggggac
      841 accatggagt acagaaaaaa gcagagaccc ctgaagatcc ggatgttaga cggaacggtg
      901 aagactatca tggtggatga ctccaagacc gtcacagaca tgctcatgac catttgtgcc
      961 cgaattggta tcaccaacca tgatgagtat tcactggttc gagagctgat ggaagaaaag
     1021 aaagatgagg ggacagggac cctgagaaaa gacaagaccc tactgcgaga tgaaaagaag
     1081 atggagaagc taaagcagaa gttgcacaca gacgatgagt tgaactggct ggaccatggg
     1141 cggacactga gggaacaggg agtggaagag catgagacgt tgctgctgcg gagaaagttc
     1201 ttctactcag accagaatgt ggattcccga gaccctgtac agctgaacct tctctatgtg
     1261 caggcacgag atgacatcct gaatggctcc catcctgtct cctttgacaa ggcctgtgaa
     1321 tttgcaggct tccagtgcca gatccagttt ggacctcaca atgaacagaa gcacaaggct
     1381 ggcttccttg acctgaagga cttcctgccc aaggagtatg tgaagcagaa gggagagcgt
     1441 aagatctttc aggcacacaa gaattgtggg cagatgagtg aaattgaggc caaggtacgc
     1501 tacgtgaagc tggcccgttc cctcaagact tacggtgtct ccttcttcct agtcaaggaa
     1561 aagatgaagg ggaagaataa actggtgccc aggctgttgg gcatcactaa ggagtgtgtg
     1621 atgcgtgtgg atgagaagac caaggaggtg attcaggaat ggagcctcac caacatcaaa
     1681 cgctgggctg cctctcccaa gagctttact ctggactttg gagactatca ggatggctac
     1741 tactcagtac agacgacaga aggcgagcag atcgcacagc tcattgctgg ctacatagat
     1801 atcatcctta agaagaaaaa aagcaaggac cattttgggc tggagggaga tgaagagtct
     1861 actatgttgg aggactcagt gtctcccaaa aagtcaacag tccttcagca gcagtacaac
     1921 cgagtgggga aagtggagca cggctctgtg gctctgccag ccatcatgcg ctctggagcc
     1981 tctggtcctg agaatttcca ggtggggagc atgccacctg cccagcagca gatcaccagt
     2041 ggccagatgc accgaggaca catgccacct ctgactagta tggtgagcaa gggcgaggag
     2101 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     2161 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     2221 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     2281 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2341 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2401 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     2461 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     2521 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     2581 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     2641 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     2701 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     2761 accggcggca tggacgagct gtacaagtaa ccgcgggccc gggatccacc ggatctagat
     2821 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2881 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     2941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     3061 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     3121 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     3181 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3241 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3301 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3361 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3421 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3481 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     3541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     3661 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3721 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     3781 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     3841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3961 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     4021 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4081 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4141 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4201 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     4261 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4321 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4381 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4441 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4501 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4561 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4621 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4681 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4741 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4801 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     4861 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     4921 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     4981 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     5041 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     5101 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     5161 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     5221 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     5281 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6121 ataaccgtat taccgccatg cat
//
