LOCUS       sequence-423004-        8150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse talin1 (1-913 + 2296-2541)-mCherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Rahikainen R, Ohman T, Turkki P, Varjosalo M, Hytonen VP
  TITLE     Talin-mediated force transmission and talin rod domain unfolding 
            independently regulate adhesion signaling.
  JOURNAL   J Cell Sci. 2019 Apr 3;132(7):jcs226514. doi: 10.1242/jcs.226514.
  PUBMED    30837291
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-3"; volume: "132(7):jcs226514. doi"; pages: " 
            10.1242/jcs.226514"
COMMENT     Sequence Label: Talin1(Delta-R4-12)-mCherry
FEATURES             Location/Qualifiers
     source          1..8150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4087..4797
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4232..4250)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4500..4519
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4938..5059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4975..4994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5029..5048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5066..5521)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5203..5224)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5415..5434
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5548..5652
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5654..6011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5654..5845
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5677..5697)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5862..5997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6046..6840
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6046..6840
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6100..6119)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6710..6729
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7028..7047)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7072..7119
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7448..8036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7937..7956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcacca tggttgcgct ttcgctgaag attagcattg ggaatgtggt gaagacgatg
      661 caatttgagc catctaccat ggtgtacgac gcctgccgca tgattcgtga gcggatccca
      721 gaggccctgg ctggccctcc caacgacttt gggctctttc tgtcagatga tgaccccaaa
      781 aaaggcatct ggctagaggc tgggaaagct ttggactact acatgctccg aaatggggac
      841 accatggagt acagaaaaaa gcagagaccc ctgaagatcc ggatgttaga cggaacggtg
      901 aagactatca tggtggatga ctccaagacc gtcacagaca tgctcatgac catttgtgcc
      961 cgaattggta tcaccaacca tgatgagtat tcactggttc gagagctgat ggaagaaaag
     1021 aaagatgagg ggacagggac cctgagaaaa gacaagaccc tactgcgaga tgaaaagaag
     1081 atggagaagc taaagcagaa gttgcacaca gacgatgagt tgaactggct ggaccatggg
     1141 cggacactga gggaacaggg agtggaagag catgagacgt tgctgctgcg gagaaagttc
     1201 ttctactcag accagaatgt ggattcccga gaccctgtac agctgaacct tctctatgtg
     1261 caggcacgag atgacatcct gaatggctcc catcctgtct cctttgacaa ggcctgtgaa
     1321 tttgcaggct tccagtgcca gatccagttt ggacctcaca atgaacagaa gcacaaggct
     1381 ggcttccttg acctgaagga cttcctgccc aaggagtatg tgaagcagaa gggagagcgt
     1441 aagatctttc aggcacacaa gaattgtggg cagatgagtg aaattgaggc caaggtacgc
     1501 tacgtgaagc tggcccgttc cctcaagact tacggtgtct ccttcttcct agtcaaggaa
     1561 aagatgaagg ggaagaataa actggtgccc aggctgttgg gcatcactaa ggagtgtgtg
     1621 atgcgtgtgg atgagaagac caaggaggtg attcaggaat ggagcctcac caacatcaaa
     1681 cgctgggctg cctctcccaa gagctttact ctggactttg gagactatca ggatggctac
     1741 tactcagtac agacgacaga aggcgagcag atcgcacagc tcattgctgg ctacatagat
     1801 atcatcctta agaagaaaaa aagcaaggac cattttgggc tggagggaga tgaagagtct
     1861 actatgttgg aggactcagt gtctcccaaa aagtcaacag tccttcagca gcagtacaac
     1921 cgagtgggga aagtggagca cggctctgtg gctctgccag ccatcatgcg ctctggagcc
     1981 tctggtcctg agaatttcca ggtggggagc atgccacctg cccagcagca gatcaccagt
     2041 ggccagatgc accgaggaca catgccacct ctgacttcag cccagcaggc gctcactgga
     2101 accattaact ccagcatgca ggctgtgcag gctgcccagg ccactctgga tgactttgag
     2161 actctacccc ctcttggcca ggatgctgcc tccaaagctt ggcgtaagaa caagatggat
     2221 gaatcaaagc atgagatcca ctcccaggta gatgccatca cagctggcac tgcctctgtg
     2281 gtaaacctga cagcaggaga tcctgcagag acagactata cagcagtggg ctgtgcagtc
     2341 accaccatct cttccaacct gacggagatg tcccgtgggg tgaagctatt ggctgccctg
     2401 ctggaggatg aaggcggcaa tggccggccc cttctgcaag cagcaaaggg ccttgcaggg
     2461 gctgtgtcgg aactgcttcg cagtgctcag cccgccagcg ctgagcctcg tcagaacctg
     2521 ctgcaggcag ccgggaacgt gggccaggcc agtggggagc tgttgcagca aattggggaa
     2581 agtgacactg acccccactt ccaggatgtt ctaatgcagc tagcaaacgc agtggcaagt
     2641 gctgcagccg ccctggtcct caaggccaag agtgtggccc agcgcacaga ggattccggg
     2701 cttcagaccc aagtgattgc tgcagctacg caatgtgctc tgtccacttc ccagctggtg
     2761 gcctgtacca aggtggtagc acctacaatc agctcacctg tgtgccaaga acagctagtc
     2821 gaggctgggc gactggtggc caaagctgtg gagggctgtg tgtctgcctc gcaagcagct
     2881 acagaagatg gacagctgct tcgaggggta ggagcagcag ccacggctgt cacccaggcc
     2941 ctcaatgagc tgctgcagca cgtgaaggcc cacgccaccg gagctgggcc tgccggtcgg
     3001 tatgaccaag ccactgatac catcctcacc gtcactgaaa acatcttcag ctccatgggt
     3061 gatgctgggg agatggtgcg ccaggcccgc atcctggccc aagccacctc agacctggtc
     3121 aatgctatca aggccgatgc tgagggggag agtgatctgg agaactctag aaagctcctg
     3181 agtgctgcca agatcctcgc tgatgccacc gccaagatgg tggaggcggc caagggagca
     3241 gccgcccacc ctgacagtga ggaacagcag cagcgactgc gtgaagcagc tgaggggctt
     3301 cgcatggcca ccaatgcagc tgcgcagaac gccatcaaga agaagttggt cgacccagag
     3361 gaccctactg tcattgctga gaatgagctc ttgggagccg cagccgccat cgaggctgca
     3421 gccaagaagc tggagcagct gaagcccagg gccaaaccca aggaggcgga tgagtccttg
     3481 aactttgagg aacaaatcct agaagctgcc aagtccatcg ctgcagccac cagtgcactg
     3541 gtaaaggctg cgtcagcagc ccagagggag ctggtggctc aaggaaaggt gggcgccatt
     3601 ccagccaatg cactggatga tgggcagtgg tcgcagggcc tcatttccgc tgcccgtatg
     3661 gtggctgcgg ccaccaacaa tctgtgtgaa gcagctaacg cagctgtcca gggccatgct
     3721 agccaggaga aactcatttc ctcagccaag caagtagctg cctccacagc tcagctcctg
     3781 gtagcttgca aggtcaaagc agatcaggac tctgaggcaa tgaaacggct ccaggctgct
     3841 ggcaatgcag tgaagagggc ctcagataac ctggtaaagg cggcccagaa agctgcagcc
     3901 ttcgaagacc aggagaatga gacggtggtg gtgaaggaga aaatggttgg gggcattgcc
     3961 cagattatcg cagcacagga agagatgctt cggaaggaac gagagctgga agaggctcgg
     4021 aaaaagctcg cccagatccg gcagcagcag tacaagttct tgccttcaga gcttcgagac
     4081 gagcacatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     4141 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     4201 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     4261 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     4321 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     4381 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     4441 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     4501 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     4561 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     4621 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     4681 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4741 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaccg
     4801 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     4861 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4921 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5041 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     5101 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5161 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5221 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5281 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5341 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5401 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5461 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5521 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5581 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5641 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5941 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6001 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6061 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6121 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6181 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6241 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6301 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6361 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6421 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6481 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6541 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6601 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6661 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6721 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6781 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6841 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6901 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6961 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     7021 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7081 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7141 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7201 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7261 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7321 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7561 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
