LOCUS       sequence-423005-        7847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse talin1 (1-490 + 1974-2541)-mCherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Rahikainen R, Ohman T, Turkki P, Varjosalo M, Hytonen VP
  TITLE     Talin-mediated force transmission and talin rod domain unfolding 
            independently regulate adhesion signaling.
  JOURNAL   J Cell Sci. 2019 Apr 3;132(7):jcs226514. doi: 10.1242/jcs.226514.
  PUBMED    30837291
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-3"; volume: "132(7):jcs226514. doi"; pages: " 
            10.1242/jcs.226514"
COMMENT     Sequence Label: Talin1(Delta-R1-10)-mCherry
FEATURES             Location/Qualifiers
     source          1..7847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3784..4494
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3929..3947)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4197..4216
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4635..4756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4672..4691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4726..4745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4763..5218)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4900..4921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5112..5131
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5245..5349
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5351..5708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5351..5542
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5374..5394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5559..5694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5743..6537
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5743..6537
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5797..5816)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6407..6426
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6725..6744)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6769..6816
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7145..7733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7634..7653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcacca tggttgcgct ttcgctgaag attagcattg ggaatgtggt gaagacgatg
      661 caatttgagc catctaccat ggtgtacgac gcctgccgca tgattcgtga gcggatccca
      721 gaggccctgg ctggccctcc caacgacttt gggctctttc tgtcagatga tgaccccaaa
      781 aaaggcatct ggctagaggc tgggaaagct ttggactact acatgctccg aaatggggac
      841 accatggagt acagaaaaaa gcagagaccc ctgaagatcc ggatgttaga cggaacggtg
      901 aagactatca tggtggatga ctccaagacc gtcacagaca tgctcatgac catttgtgcc
      961 cgaattggta tcaccaacca tgatgagtat tcactggttc gagagctgat ggaagaaaag
     1021 aaagatgagg ggacagggac cctgagaaaa gacaagaccc tactgcgaga tgaaaagaag
     1081 atggagaagc taaagcagaa gttgcacaca gacgatgagt tgaactggct ggaccatggg
     1141 cggacactga gggaacaggg agtggaagag catgagacgt tgctgctgcg gagaaagttc
     1201 ttctactcag accagaatgt ggattcccga gaccctgtac agctgaacct tctctatgtg
     1261 caggcacgag atgacatcct gaatggctcc catcctgtct cctttgacaa ggcctgtgaa
     1321 tttgcaggct tccagtgcca gatccagttt ggacctcaca atgaacagaa gcacaaggct
     1381 ggcttccttg acctgaagga cttcctgccc aaggagtatg tgaagcagaa gggagagcgt
     1441 aagatctttc aggcacacaa gaattgtggg cagatgagtg aaattgaggc caaggtacgc
     1501 tacgtgaagc tggcccgttc cctcaagact tacggtgtct ccttcttcct agtcaaggaa
     1561 aagatgaagg ggaagaataa actggtgccc aggctgttgg gcatcactaa ggagtgtgtg
     1621 atgcgtgtgg atgagaagac caaggaggtg attcaggaat ggagcctcac caacatcaaa
     1681 cgctgggctg cctctcccaa gagctttact ctggactttg gagactatca ggatggctac
     1741 tactcagtac agacgacaga aggcgagcag atcgcacagc tcattgctgg ctacatagat
     1801 atcatcctta agaagaaaaa aagcaaggac cattttgggc tggagggaga tgaagagtct
     1861 actatgttgg aggactcagt gtctcccaaa aagtcaacag tccttcagca gcagtacaac
     1921 cgagtgggga aagtggagca cggctctgtg gctctgccag ccatcatgcg ctctggagcc
     1981 tctggtcctg agaatttcca ggtggggagc atgccacctg cccagcagca gatcaccagt
     2041 ggccagatgc accgaggaca catgccacct ctgactagtg gtacccaggc ctgcattaca
     2101 gcagccagtg ctgtgtctgg tatcattgct gacctcgaca ccaccatcat gttcgctact
     2161 gctggcacac ttaaccgtga gggtgctgaa acttttgctg accaccggga gggtatctta
     2221 aagacagcaa aggttcttgt ggaggacacc aaggtcctag tgcagaatgc agctgggagc
     2281 caggagaagt tggcacaagc cgcccagtcc tccgtggcca ccattacccg cctcgctgat
     2341 gtggtcaagc tcggtgcagc cagcctagga gccgaagacc ctgaaactca ggtggtgctg
     2401 atcaatgcag taaaggacgt agccaaggcc ctgggtgacc tcatcagcgc tacgaaggct
     2461 gcagcgggca aagttgggga tgaccctgca gtgtggcagc tcaagaactc tgccaaggtg
     2521 atggtgacca atgtgacatc attgctcaag acagtgaagg ctgtggaaga tgaggccacc
     2581 aaaggcacac gggccctaga ggcaaccaca gagcacatac gtcaggaact ggcggtcttc
     2641 tgttccccag agccacctgc caagacctct acccctgaag acttcatccg aatgaccaag
     2701 ggtattacta tggcaacagc caaagccgtt gctgctggca attcctgtcg acaggaagat
     2761 gtcattgcca cagccaatct gagccgacgg gctattgcgg acatgcttcg ggcttgcaag
     2821 gaagcagctt tccacccaga agtggcgcct gatgtacggc tccgagccct gcactatggc
     2881 cgggagtgtg ccaatggtta cctggaactg ctggaccacg tgctgctgac ccttcagaag
     2941 ccaaacccag acctgaagca gcagctaact ggacactcaa agcgggttgc tggctctgtg
     3001 actgagctca tccaggctgc tgaagccatg aaaggaacag agtgggtgga cccagaggac
     3061 cctactgtca ttgctgagaa tgagctcttg ggagccgcag ccgccatcga ggctgcagcc
     3121 aagaagctgg agcagctgaa gcccagggcc aaacccaagg aggcggatga gtccttgaac
     3181 tttgaggaac aaatcctaga agctgccaag tccatcgctg cagccaccag tgcactggta
     3241 aaggctgcgt cagcagccca gagggagctg gtggctcaag gaaaggtggg cgccattcca
     3301 gccaatgcac tggatgatgg gcagtggtcg cagggcctca tttccgctgc ccgtatggtg
     3361 gctgcggcca ccaacaatct gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc
     3421 caggagaaac tcatttcctc agccaagcaa gtagctgcct ccacagctca gctcctggta
     3481 gcttgcaagg tcaaagcaga tcaggactct gaggcaatga aacggctcca ggctgctggc
     3541 aatgcagtga agagggcctc agataacctg gtaaaggcgg cccagaaagc tgcagccttc
     3601 gaagaccagg agaatgagac ggtggtggtg aaggagaaaa tggttggggg cattgcccag
     3661 attatcgcag cacaggaaga gatgcttcgg aaggaacgag agctggaaga ggctcggaaa
     3721 aagctcgccc agatccggca gcagcagtac aagttcttgc cttcagagct tcgagacgag
     3781 cacatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     3841 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     3901 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3961 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     4021 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     4081 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     4141 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4201 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4261 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4321 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4381 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4441 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaccgcgg
     4501 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     4561 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4621 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4681 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4741 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     4801 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4861 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4921 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4981 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5041 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5101 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5161 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     5221 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5281 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5341 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     5401 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5461 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5521 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5581 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5641 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5701 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5761 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5821 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5881 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     5941 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6001 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6061 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6121 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6181 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6241 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     6301 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6361 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6421 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6481 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6541 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6601 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6661 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     6721 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     6781 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     6841 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     6901 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     6961 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     7021 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7261 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7741 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7801 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
