LOCUS       sequence-423007-        6881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse talin1 (1-490 + 2296-2541)-mCherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Rahikainen R, Ohman T, Turkki P, Varjosalo M, Hytonen VP
  TITLE     Talin-mediated force transmission and talin rod domain unfolding 
            independently regulate adhesion signaling.
  JOURNAL   J Cell Sci. 2019 Apr 3;132(7):jcs226514. doi: 10.1242/jcs.226514.
  PUBMED    30837291
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-04-3"; volume: "132(7):jcs226514. doi"; pages: " 
            10.1242/jcs.226514"
COMMENT     Sequence Label: Talin1(Delta-R1-12)-mCherry
FEATURES             Location/Qualifiers
     source          1..6881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2818..3528
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2963..2981)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3231..3250
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3669..3790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3706..3725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3760..3779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3797..4252)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3934..3955)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4146..4165
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4279..4383
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4385..4742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4385..4576
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4408..4428)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4593..4728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4777..5571
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4777..5571
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4831..4850)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5441..5460
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5759..5778)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5803..5850
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6179..6767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6668..6687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcacca tggttgcgct ttcgctgaag attagcattg ggaatgtggt gaagacgatg
      661 caatttgagc catctaccat ggtgtacgac gcctgccgca tgattcgtga gcggatccca
      721 gaggccctgg ctggccctcc caacgacttt gggctctttc tgtcagatga tgaccccaaa
      781 aaaggcatct ggctagaggc tgggaaagct ttggactact acatgctccg aaatggggac
      841 accatggagt acagaaaaaa gcagagaccc ctgaagatcc ggatgttaga cggaacggtg
      901 aagactatca tggtggatga ctccaagacc gtcacagaca tgctcatgac catttgtgcc
      961 cgaattggta tcaccaacca tgatgagtat tcactggttc gagagctgat ggaagaaaag
     1021 aaagatgagg ggacagggac cctgagaaaa gacaagaccc tactgcgaga tgaaaagaag
     1081 atggagaagc taaagcagaa gttgcacaca gacgatgagt tgaactggct ggaccatggg
     1141 cggacactga gggaacaggg agtggaagag catgagacgt tgctgctgcg gagaaagttc
     1201 ttctactcag accagaatgt ggattcccga gaccctgtac agctgaacct tctctatgtg
     1261 caggcacgag atgacatcct gaatggctcc catcctgtct cctttgacaa ggcctgtgaa
     1321 tttgcaggct tccagtgcca gatccagttt ggacctcaca atgaacagaa gcacaaggct
     1381 ggcttccttg acctgaagga cttcctgccc aaggagtatg tgaagcagaa gggagagcgt
     1441 aagatctttc aggcacacaa gaattgtggg cagatgagtg aaattgaggc caaggtacgc
     1501 tacgtgaagc tggcccgttc cctcaagact tacggtgtct ccttcttcct agtcaaggaa
     1561 aagatgaagg ggaagaataa actggtgccc aggctgttgg gcatcactaa ggagtgtgtg
     1621 atgcgtgtgg atgagaagac caaggaggtg attcaggaat ggagcctcac caacatcaaa
     1681 cgctgggctg cctctcccaa gagctttact ctggactttg gagactatca ggatggctac
     1741 tactcagtac agacgacaga aggcgagcag atcgcacagc tcattgctgg ctacatagat
     1801 atcatcctta agaagaaaaa aagcaaggac cattttgggc tggagggaga tgaagagtct
     1861 actatgttgg aggactcagt gtctcccaaa aagtcaacag tccttcagca gcagtacaac
     1921 cgagtgggga aagtggagca cggctctgtg gctctgccag ccatcatgcg ctctggagcc
     1981 tctggtcctg agaatttcca ggtggggagc atgccacctg cccagcagca gatcaccagt
     2041 ggccagatgc accgaggaca catgccacct ctgactagtg tggacccaga ggaccctact
     2101 gtcattgctg agaatgagct cttgggagcc gcagccgcca tcgaggctgc agccaagaag
     2161 ctggagcagc tgaagcccag ggccaaaccc aaggaggcgg atgagtcctt gaactttgag
     2221 gaacaaatcc tagaagctgc caagtccatc gctgcagcca ccagtgcact ggtaaaggct
     2281 gcgtcagcag cccagaggga gctggtggct caaggaaagg tgggcgccat tccagccaat
     2341 gcactggatg atgggcagtg gtcgcagggc ctcatttccg ctgcccgtat ggtggctgcg
     2401 gccaccaaca atctgtgtga agcagctaac gcagctgtcc agggccatgc tagccaggag
     2461 aaactcattt cctcagccaa gcaagtagct gcctccacag ctcagctcct ggtagcttgc
     2521 aaggtcaaag cagatcagga ctctgaggca atgaaacggc tccaggctgc tggcaatgca
     2581 gtgaagaggg cctcagataa cctggtaaag gcggcccaga aagctgcagc cttcgaagac
     2641 caggagaatg agacggtggt ggtgaaggag aaaatggttg ggggcattgc ccagattatc
     2701 gcagcacagg aagagatgct tcggaaggaa cgagagctgg aagaggctcg gaaaaagctc
     2761 gcccagatcc ggcagcagca gtacaagttc ttgccttcag agcttcgaga cgagcacatg
     2821 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2881 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2941 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     3001 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     3061 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     3121 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     3181 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3241 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3301 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3361 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3421 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3481 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaacc gcgggcccgg
     3541 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     3601 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3661 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3781 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3841 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3901 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3961 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4021 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4081 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4141 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4201 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     4261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4381 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4741 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4801 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4861 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4921 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     4981 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5041 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5101 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5161 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5221 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5281 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     5341 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5401 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5461 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5521 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5581 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5641 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5701 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     5761 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     5821 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5881 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     5941 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     6001 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     6061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6841 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
