LOCUS       sequence-42303-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00419
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(557..656)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(672..689)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(672..688)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(681..703)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagtt ggcaatgcaa agcttttcac aactttggtg
      121 gacgaaatgg acttatgaca ccgcggacac ttgatgcagg tttccggatc tagaagtcct
      181 ttgatcttgt gcagacacgg atgtatctgc gttctcacgt gttcctttgc tgcgattggc
      241 tcggcaatat ccaaccgctg gtggagtata ttgtaggcct ccttccataa acttgatttg
      301 tagctctgtt ttctgggctc actgtattca taaacacgaa tgccattttt tactcgaaca
      361 gcatgcggat acggcggaat gctgcgcgaa agtttcctag ttataatttt gccttcagca
      421 gaagcgtaag ctgaatccaa aatatttcgt acacttaatt tggaatccgg catgtgcgaa
      481 acgtgttttt cgtcgtcgta taaatattcg tatggtagat cagaactatg aaaaactacc
      541 ttgtccccct ccatggtgcc aacttttttg tacaaagttg gcattataaa aaagcattgc
      601 tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggggc
      661 ccgagcttaa gactggccgt cgttttacaa cgtcgtgact gggaaaacat ccntgctagc
      721 gttaacgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
//
