LOCUS       sequence-42305-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HECTD1
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(455..473)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      469..516
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(640..659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atataccatg catcatcatc atcatcacag
       61 cagcggcaga gaaaacttgt atttccaggg catgaaggat tctgataaag aaaaggaaaa
      121 tggaaaaatg ggttgctggt ctatagagca tgtggagcag taccttggca ctgatgaatt
      181 accaaagaat gacttgataa cctacctgca gaagaatgca gacgctgctt tcctgcgcca
      241 ctggaaatta actggcacta ataaaagtat taggaaaaac agaaattgtt ctcagctcat
      301 agctgcatat aaggattttt gtgagcatgg aacaaagtct gggttaaacc aggggtgatg
      361 acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      421 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      481 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      541 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      781 cacgtagtgg gccatcg
//
