LOCUS       sequence-423075-        9917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of the PE2 proteins for genome editing in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Zhang H, Ma J, Wu Z, Chen X, Qian Y, Chen W, Wang Z, Zhang Y, Zhu H,
            Huang X, Ji Q
  TITLE     BacPE: a versatile prime-editing platform in bacteria by inhibiting 
            DNA exonucleases.
  JOURNAL   Nat Commun. 2024 Jan 27;15(1):825. doi: 10.1038/s41467-024-45114-4.
  PUBMED    38280845
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-01-27"; volume: "15(1):825. doi"; pages: " 
            10.1038/s41467-024-45114-4"
COMMENT     Sequence Label: Sp_PE
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(37..915)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        942..1226
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1149..1168
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1253..1261
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             join(1270..3786,3787..3789,3790..5373)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      7549..7793
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     rep_origin      complement(7821..8366)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7901..7920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8952..9743,9744..9812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9575..9594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9813..9917)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgcatc gatttattat gacaacttga cggctacatc
       61 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
      121 aaatacccgc gagaagtaga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
      181 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
      241 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
      301 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
      361 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
      421 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
      481 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
      541 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
      601 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
      661 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
      721 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
      781 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
      841 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
      901 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
      961 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     1021 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     1081 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     1141 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     1201 atcgcaactc tctactgttt ctccataccc gtttttttgg gaattcgagc tctaaggagg
     1261 ttataaaaaa tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga
     1321 tgggcggtga tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat
     1381 acagaccgcc acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag
     1441 acagcggaag cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat
     1501 cgtatttgtt atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc
     1561 tttcatcgac ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct
     1621 atttttggaa atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat
     1681 ctgcgaaaaa aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc
     1741 ttagcgcata tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat
     1801 aatagtgatg tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa
     1861 gaaaacccta ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt
     1921 aaatcaagac gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta
     1981 tttgggaatc tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat
     2041 ttggcagaag atgctaaatt acagctttca aaagatactt acgatgatga tttagataat
     2101 ttattggcgc aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca
     2161 gatgctattt tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta
     2221 tcagcttcaa tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct
     2281 ttagttcgac aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac
     2341 ggatatgcag gttatattga tgggggagct agccaagaag aattttataa atttatcaaa
     2401 ccaattttag aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat
     2461 ttgctgcgca agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt
     2521 gagctgcatg ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt
     2581 gagaagattg aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt
     2641 ggcaatagtc gttttgcatg gatgactcgg aaatctgaag aaacaattac cccatggaat
     2701 tttgaagaag ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac
     2761 tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat
     2821 tttacggttt ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca
     2881 gcatttcttt caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga
     2941 aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt
     3001 gttgaaattt caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg
     3061 ctaaaaatta ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag
     3121 gatattgttt taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa
     3181 acatatgctc acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact
     3241 ggttggggac gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa
     3301 acaatattag attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc
     3361 catgatgata gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc
     3421 gatagtttac atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt
     3481 ttacagactg taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa
     3541 aatatcgtta ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg
     3601 cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa
     3661 gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa
     3721 aatggaagag acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat
     3781 gtcgatgcta ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta
     3841 acgcgttctg ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa
     3901 aagatgaaaa actattggag acaacttcta aacgccaagt taatcactca acgtaagttt
     3961 gataatttaa cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc
     4021 aaacgccaat tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt
     4081 cgcatgaata ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc
     4141 ttaaaatcta aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag
     4201 attaacaatt accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg
     4261 attaagaaat atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat
     4321 gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc
     4381 ttttactcta atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt
     4441 cgcaaacgcc ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg
     4501 cgagattttg ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa
     4561 acagaagtac agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac
     4621 aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca
     4681 acggtagctt attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta
     4741 aaatccgtta aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat
     4801 ccgattgact ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa
     4861 ctacctaaat atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc
     4921 ggagaattac aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat
     4981 ttagctagtc attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg
     5041 tttgtggagc agcataagca ttatttagat gagattattg agcaaatcag tgaattttct
     5101 aagcgtgtta ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat
     5161 agagacaaac caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat
     5221 cttggagctc ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg
     5281 tctacaaaag aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa
     5341 acacgcattg atttgagtca gctaggaggt gacagcggcg gttcttccgg cggctccagc
     5401 ggttccgaaa ctccgggtac ctctgaatct gcgaccccgg aatcttctgg cggcagctct
     5461 ggcggtagca gcaccctgaa catcgaagat gaatatcgtc tgcacgaaac ctctaaagag
     5521 ccggacgttt ccctgggcag cacctggctg tccgatttcc cgcaggcgtg ggcggaaacc
     5581 ggcggcatgg gtctggcggt tcgccaggcg ccgctgatca tcccgctgaa agcgacctct
     5641 accccggtaa gcatcaaaca gtatccgatg agccaggaag cgcgtttagg tatcaaaccg
     5701 catattcagc gcctgctgga tcagggtatt ctggtgccgt gccagtctcc gtggaacact
     5761 ccactgcttc cggtcaaaaa accgggtacc aacgactacc gtccggtaca ggatctgcgt
     5821 gaagttaaca aacgtgttga agatatccat ccgaccgtac cgaacccgta taacctgctg
     5881 tctggcctgc cgccgagcca ccagtggtac accgtgctgg atttgaaaga tgcgtttttc
     5941 tgtctgcgcc tgcatccgac ctcccaaccg ctgttcgcct tcgaatggcg tgatccggag
     6001 atgggtatta gcggccagct gacttggact cgtctgccgc agggcttcaa aaacagcccg
     6061 acgctcttca acgaagcgct gcatcgtgac ctggccgatt tccgtatcca gcacccggat
     6121 ctgatcttgc tgcagtacgt ggacgacctg ctgctggcgg caacgtcgga actggactgt
     6181 cagcagggca ctcgtgcact gctgcagacc ctgggtaacc tgggttatcg tgcatctgct
     6241 aaaaaagccc agatctgtca gaaacaggtt aaatacctcg gctacttgct gaaagaaggc
     6301 cagcgctggc ttaccgaagc tcgtaaagag actgtgatgg gtcagccgac gccgaaaacc
     6361 ccgcgccaac tgcgtgaatt cctgggtaaa gctggcttct gccgtctgtt cattccgggc
     6421 tttgctgaaa tggcggctcc gctgtatcca ctgaccaaac cgggtaccct gttcaactgg
     6481 ggccctgacc agcagaaagc ataccaggaa attaaacagg ctcttctgac cgcgccggca
     6541 ctgggcctgc cggatctgac caaaccgttc gaactgtttg ttgatgaaaa acagggttac
     6601 gcgaaaggcg tcctgaccca gaaactgggt ccgtggcgcc gcccggtggc gtacctgagc
     6661 aaaaaactgg atccggttgc agcaggttgg ccgccgtgcc tgcgcatggt tgctgcgatt
     6721 gctgtgttga ccaaagatgc tggtaaactg accatgggtc agccgctggt gatcctggct
     6781 ccgcacgctg ttgaagcgct ggtaaaacag ccgccggacc gctggttgtc taacgcccgt
     6841 atgacccact atcaggctct gctgctggac accgaccgtg ttcagttcgg cccagtggtg
     6901 gcactgaatc cagcgaccct gctgccactg ccggaagagg gtctgcagca caactgcctt
     6961 gatatcctgg ccgaagcgca cggtacccgt ccggacttaa ccgatcagcc gctgccggat
     7021 gctgatcata cctggtatac cgacggttcc tctttgttgc aggaaggtca gcgtaaagcg
     7081 ggtgcggccg ttaccaccga aactgaagta atttgggcca aggcgctccc ggcgggcacg
     7141 tccgcgcagc gcgctgaact catcgcactg acccaggcgt tgaaaatggc agaaggcaaa
     7201 aaactgaatg tgtacactga ttcccgctac gcgttcgcaa ccgctcacat ccacggcgaa
     7261 atctaccgcc gtcgcggctg gctgactagc gaaggtaaag aaattaaaaa taaagatgaa
     7321 atcctggcac tgctgaaagc actgttcctg ccgaaacgtc tgtctatcat tcactgccca
     7381 ggccaccaga agggccactc cgctgaagca cgtggtaacc gcatggctga tcaggcagca
     7441 cgtaaagcgg caattaccga aaccccggac acctctaccc tgctgatcga aaactcttcg
     7501 ccgagcggtg gctctaaacg taccgctgac ggctccgaat ttgaataacg catcctcacg
     7561 ataatatccg ggtaggcgca atcactttcg tctactccgt tacaaagcga ggctgggtat
     7621 ttcccggcct ttctgttatc cgaaatccac tgaaagcaca gcggctggct gaggagataa
     7681 ataataaacg aggggctgta tgcacaaagc atcttctgtt gagttaagaa cgagtatcga
     7741 gatggcacat agccttgctc aaattggaat caggtttgtg ccaataccag tagaaacaga
     7801 cgaagaatcc atgggtatgg tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     7861 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7921 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     7981 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     8041 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     8101 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     8161 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     8221 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     8281 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     8341 ttacgcgcag accaaaacga tctcaagtcg caaacgctgt ttgctcctct acaaaacaga
     8401 ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cggttgcggc cgcaatcggg
     8461 caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc
     8521 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca
     8581 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg
     8641 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc
     8701 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca
     8761 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacggggtc tgacgctcag
     8821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9901 caaatagggg ttccgcg
//
