LOCUS       sequence-423100-       11029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-Off controlled GFP-Pho8 with TRP1 selection. 
            Tetracycline-controlled transactivator (tTA) present on same 
            plasmid. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, 
            Gong Q, Xie Z
  TITLE     Membrane recruitment of Atg8 by Hfl1 facilitates turnover of 
            vacuolar membrane proteins in yeast cells approaching stationary 
            phase.
  JOURNAL   BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7.
  PUBMED    34088313
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2021-06-4"; volume: "19(1):117. doi"; pages: " 
            10.1186/s12915-021-01048-7"
COMMENT     Sequence Label: BS-TRP1-TetOff(7repeats)-GFP-Pho8
FEATURES             Location/Qualifiers
     source          1..11029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5..21
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     40..60
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        42..60
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        796..1073
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     1810..1826
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1811..1827
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(1863..2249,2250..2870))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     2778..2797
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(2955..2979)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2976..3179)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3005..3025)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3180..3559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      3671..3858
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3726..3750)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(3916..3934,3935..3957,3958..3976,3977..3999,4000..4018,
                     4019..4041,4042..4060,4061..4083,4084..4102,4103..4125,
                     4126..4144,4145..4167,4168..4186)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             4411..5124
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     primer_bind     complement(7216..7232)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7216..7232)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8259..8278)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8260..8278)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8285..8302)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8285..8301)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8294..8316)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8443..8898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8530..8549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8740..8761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8924..9028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9029..9097,9098..9889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9247..9266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10060..10648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10549..10568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10802..10819
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10936..10957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10972..10977,10978..10995,10996..11002)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11010..11026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(11015..11029,1..8)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
       61 gaacaaaagc tggtactatt agctgaattg ccactgctat cgttgttagt ggcgttagtg
      121 cttgcattca aagacatgga gggcgttatt acgccggagc tcctcgacag cagatctgat
      181 gactggtcaa tatatttttg cattgaggct ctgtttggaa ttatattttg agatgaccca
      241 tctaatgtac tggtatcacc agatttcatg tcgtttttta aagcggctgc ttgagtctta
      301 gcaatagcgt caccatctgg tgaatccttt gaaggaacca ctgacgaagg tttggacagt
      361 gacgaagagg atctttcctg ctttgaatta gtcgcgctgg gagcagatga cgagttggtg
      421 gagctggggg caggattgct ggccgtcgtg ggtcctgaat gggtccttgg ctggtccatc
      481 tctattctga aaacggaaga ggagtaggga atattactgg ctgaaaataa gtcttgaatg
      541 aacgtatacg cgtatatttc taccaatctc tcaacactga gtaatggtag ttataagaaa
      601 gagaccgagt tagggacagt tagaggcggt ggagatattc cttatggcat gtctggcgat
      661 gataaaactt ttcaaacggc agccccgatc taaaagagct gacagggaaa tggtcagaaa
      721 aagaaacgtg cacccgcccg tctggacgcg ccgctcaccc gcacggcaga gaccaatcag
      781 taaaaatcaa cggttaacga cattactata tatataatat aggaagcatt taatagaaca
      841 gcatcgtaat atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt
      901 ctttattgaa aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt
      961 tgccgattaa gaattcggtc gaaaaaagaa aaggagaggg ccaagaggga gggcattggt
     1021 gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg agtatgctcg
     1081 ttaaagtgtg tggtttgcaa accgttgaag ctgcaaagac tgctgtggat gatggtgctg
     1141 attacttagg tatcatttgt gttcccggta ggaaaagaac cattgactca tctgttgcga
     1201 aaggtatttc aactgcagtt caccaacaag agaacgtgaa aggtactaaa ctagtcgggg
     1261 tgtttagaaa tcagtccgtt gatgatgtcc ttcaactgta ccacgaatat aatctagatg
     1321 tgatacaatt acatggagat gaagatatta aagaatacag atctttgatc ccatcttcaa
     1381 ttccaatcat taagaggttc cagttcccac aggattgtga attactactg gacctgtatg
     1441 aacacgtaga caatgtgctg acgttgttcg attctggtga aggtggcact ggtgagaaat
     1501 tgaattggag tgcaatttcc agttggtctg caagtcatcc cgagataaaa ttcattatcg
     1561 ctggtggatt gaatcctgat aacgtttctg ttgccattaa tatgttacca aatgcgatcg
     1621 gtgtcgatgt aagtggagga gtagagactg atggtatcaa ggatttagaa aaggtaaagc
     1681 tattcatcca gcaggcctct caatagagga aaaaacgtat ctttcttcaa ttacacatac
     1741 gtatatataa attgatatat atatagtgaa tgatcgttcc gcggccgctc tagggtaccg
     1801 ggccccccct cgaggtcgac ggtatcgata agcttgaatt cttattacga tcctcgcgcc
     1861 ccctacccac cgtactcgtc aattccaagg gcatcggtaa acatctgctc aaactcgaag
     1921 tcggccatat ccagagcgcc gtagggggcg gagtcgtggg gggtaaatcc cggacccggg
     1981 gaatccccgt cccccaacat gtccagatcg aaatcgtcta gcgcgtcggc atgcgccatc
     2041 gccacgtcct cgccgtctaa gtggagctcg tcccccaggc tgacatcggt cgggggggcc
     2101 gtcgacagtc tgcgcgtgtg tcccgcgggg agaaaggaca ggcgcggagc cgccagcccc
     2161 gcctcttcgg gggcgtcgtc gtccgggaga tcgagcaggc cctcgatggt agacccgtaa
     2221 ttgtttttcg tacgcgcgcg gctgtacgcg gacccacttt cacatttaag ttgtttttct
     2281 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     2341 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     2401 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     2461 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     2521 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     2581 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     2641 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     2701 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     2761 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     2821 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat atgaattaat
     2881 tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga
     2941 tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata gacctcccac
     3001 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     3061 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
     3121 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
     3181 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     3241 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     3301 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     3361 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     3421 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     3481 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     3541 ctagtcaata atcaatgtca acatggcggt aatgttggac atgagccaat ataaatgtac
     3601 atattatgat atggatacaa cgtatgcaat gggccaagct cctcgagtaa ttcgcgccac
     3661 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     3721 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     3781 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat
     3841 tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga tcaattcctc
     3901 gatcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     3961 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     4021 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     4081 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     4141 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     4201 gctcggtacc ctatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt
     4261 ttctctaaat attctttcct tatacattag gtcctttgta gcataaatta ctatacttct
     4321 atagacacgc aaacacaaat acacacacta aattaccgga tcaattcggg ggatccgttt
     4381 aaacaggcct gttaacatcg atagcggccg atgtctaaag gtgaagaatt attcactggt
     4441 gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc
     4501 ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact
     4561 ggtaaattgc cagttccatg gccaacctta gtcactactt taacttatgg tgttcaatgt
     4621 ttttctagat acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa
     4681 ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct
     4741 gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt
     4801 aaagaagatg gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt
     4861 tacatcatgg ctgacaaaca aaagaatggt atcaaagtta acttcaaaat tagacacaac
     4921 attgaagatg gttctgttca attagctgac cattatcaac aaaatactcc aattggtgat
     4981 ggtccagtct tgttaccaga caaccattac ttatccactc aatctgcctt atccaaagat
     5041 ccaaacgaaa agagagacca catggtcttg ttagaatttg ttactgctgc tggtattacc
     5101 catggtattg atgaattgta caaaggagcc ggaacaagtg acatgatgac tcacacatta
     5161 ccaagcgaac agacacgtct tgttcctgga tctgactcga gctctcgtcc taaaaagaga
     5221 cggatctcga agagatcaaa gataatagta tccactgtgg tctgtattgg tttgttgctg
     5281 gttttagtgc aactggcatt tccaagcagt tttgcattac gttctgcatc acacaagaag
     5341 aagaatgtca tattcttcgt gacggatggg atgggacctg cgtctttgtc catggcaaga
     5401 tcattcaacc agcatgttaa tgatttacct atagacgaca tcctgacact tgacgagcat
     5461 tttatcgggt cttcgagaac aaggtcctcg gactcactag tcactgactc agctgcgggc
     5521 gccaccgcat ttgcatgtgc attaaagagc tataatgggg ccatcggtgt tgatccgcat
     5581 cacagacctt gtggtacagt gctcgaggcg gctaagttag caggttatct cactgggctt
     5641 gttgttacga caagaatcac agatgccact ccggcttcat tcagttcaca tgttgattac
     5701 agatggcagg aagatcttat cgctacgcac cagttaggcg aatatcctct ggggagagta
     5761 gtagacttac tcatgggtgg cggcaggagc catttctacc ctcaaggcga aaaggcttct
     5821 ccatatggtc accacggtgc tagaaaagat ggaagagatt taatcgatga ggctcaatct
     5881 aatggctggc agtacgtggg tgaccgtaaa aattttgatt ctttgttgaa aagccacggt
     5941 gaaaacgtta ctttaccttt cttgggtctc tttgcagata acgacattcc ttttgaaatt
     6001 gacagagatg aaaaggagta tccttccctt aaggaacaag tcaaggtggc tttgggtgcc
     6061 ttggaaaaag cttccaatga ggacaaagat tctaatgggt ttttcttaat ggtggaaggc
     6121 tccagaattg atcatgctgg tcaccaaaac gatccagctt cgcaggtaag ggaggtgttg
     6181 gctttcgatg aagcattcca atacgttttg gagtttgctg aaaattccga tacggagact
     6241 gttttggtct ctacgtcgga tcatgaaacg ggtggtttag tcacttcaag acaagtcact
     6301 gctagctatc ctcagtatgt ctggtatcca caggtgctcg ccaacgccac ccattcaggt
     6361 gagtttttga aaagaaaact agtagatttt gttcatgagc ataagggcgc aagcagcaaa
     6421 atagaaaatt tcatcaaaca tgagatcctg gaaaaagatt tgggtattta cgattacacg
     6481 gatagcgatt tagaaacatt aattcatttg gatgacaacg caaatgctat ccaagacaaa
     6541 ctgaatgaca tggtatcatt tagggcccaa attggctgga ccacgcatgg tcacagcgca
     6601 gtcgacgtca acatatatgc atacgccaat aagaaagcta catggtcata tgtcttgaac
     6661 aatttacaag gcaatcacga aaatacggaa gttggccagt tcttagaaaa ctttttagaa
     6721 ttgaacctga acgaagtcac tgatttgatt agggacacca aacacacatc agattttgat
     6781 gctacagaaa tagctagtga agtacaacat tatgacgaat actaccatga gttgaccaac
     6841 tgatcctatc taactctccc tcttttttca catattattt aatacgttat aatttatttg
     6901 aatatatata tatatatata tgtatatata ctaaccgcat gacacgtact gtatcgcatt
     6961 tcgtttcgga gtgcaggcga gttttaggtg cattctatag cgtgaagaag tgaaatctcg
     7021 tcatttcttc aagttatgcc attcagtcca gattgtttca catgctttca tatagtcatt
     7081 tttgtcgtcg cgaacttcaa atttgaaaac atcatcatac agagagattt ctccaataat
     7141 gatcctttct ttgtcgttgt catcatcggc atcgtcgtta agtgaagctt gatatcgaat
     7201 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg ggttattact
     7261 gagtagtatt tatttaagta ttgtttgtgc acttgcctgc aggccttttg aaaagcaagc
     7321 ataaaagatc taaacataaa atctgtaaaa taacaagatg taaagataat gctaaatcat
     7381 ttggcttttt gattgattgt acaggaaaat atacatcgca gggggttgac ttttaccatt
     7441 tcaccgcaat ggaatcaaac ttgttgaaga gaatgttcac aggcgcatac gctacaatga
     7501 cccgattctt gctagccttt tctcggtctt gcaaacaacc gccggcagct tagtatataa
     7561 atacacatgt acatacctct ctccgtatcc tcgtaatcat tttcttgtat ttatcgtctt
     7621 ttcgctgtaa aaactttatc acacttatct caaatacact tattaaccgc ttttactatt
     7681 atcttctacg ctgacagtaa tatcaaacag tgacacatat taaacacagt ggtttctttg
     7741 cataaacacc atcagcctca agtcgtcaag taaagatttc gtgttcatgc agatagataa
     7801 caatctatat gttgataatt agcgttgcct catcaatgcg agatccgttt aaccggaccc
     7861 tagtgcactt accccacgtt cggtccactg tgtgccgaac atgctccttc actattttaa
     7921 catgtggaat tcttgaaaga atgaaatcgc catgccaagc catcacacgg tcttttatgc
     7981 aattgattga ccgcctgcaa cacataggca gtaaaatttt tactgaaacg tatataatca
     8041 tcataagcga caagtgaggc aacacctttg ttaccacatt gacaacccca ggtattcata
     8101 cttcctatta gcggaatcag gagtgcaaaa agagaaaata aaagtaaaaa ggtagggcaa
     8161 cacatagtat gaatacaaac gttccaatat tcagttctcc ggtcagagat ttaccaaggt
     8221 ctttcgaaca aaaacattta gcggttgtag acaattcgcc ctatagtgag tcgtattaca
     8281 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8341 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8401 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     8461 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8521 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8581 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8641 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8701 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8761 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8821 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8881 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8941 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9001 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9061 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9901 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9961 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10201 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10441 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10681 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10741 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10801 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    10861 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10921 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10981 tatgctcccg gctcgtatgt tgtgtggaat tgtgagcgga taacaattt
//
