LOCUS       sequence-423109-       13314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for DOX-inducible NUDT18 gene expression with 
            C-terminal FLAG tag. Gateway expression system. Selection by 
            puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13314)
  TITLE     Chromosome 8p-engineering reveals increased metastasis targetable by
            patient-specific synthetic lethality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ-NUDT18-C-FLAG-Puro
FEATURES             Location/Qualifiers
     source          1..13314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    696..821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1318..1551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1736..1780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2078..2195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2260..2634)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2631..2640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2644..2662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2654..2701)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2737..2755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2772..2790
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2808..2826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2844..2862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2879..2897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2915..2933
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2945..2983
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2980..3004
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3017..3041
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4011..4035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4046..4069
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4079..5290
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5127..5146
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5303..5336)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             5343..6050
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(6051..6084)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6107..6679
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6274..6291)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6501..6520
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6682..7284
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7181..7201
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7300..7888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7353..7373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7771..7782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8095..8328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8429..8446)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8435..8659
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8705..9133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8792..8811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9002..9023
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9142..9162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9147..9476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9327..9462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9389..9408
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10684..10805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10854..10870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10854..10870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10867..10889)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10878..10894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10902..10908,10909..10926,10927..10932))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10947..10968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11085..11102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11256..11844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11336..11355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12015..12806,12807..12875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12638..12657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12876..12980)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13028..13162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
       61 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      121 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      181 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      241 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      301 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      361 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      421 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1141 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1201 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1261 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1321 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1381 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1441 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1501 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1561 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1621 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1681 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1741 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1801 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1861 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1921 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1981 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     2041 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2101 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2161 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2221 atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct cctcggccac
     2281 gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc
     2341 gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga
     2401 ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg
     2461 caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc
     2521 gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc
     2581 tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatggtggc
     2641 cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
     2701 cacgtgctgc aggtccgagg ttctagacga gtttactccc tatcagtgat agagaacgat
     2761 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2821 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     2881 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2941 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     3001 gatcgcaccg gtatcaacaa gtttgtacaa aaaagttggc atgacctcgg agggcctggc
     3061 gggggcgctg gcttccgtgc tggctggcca ggggtccagc gtgcacagct gcgactcggc
     3121 gccggccggg gagccgccgg cgcccgtgcg gctgcggaag aacgtgtgct acgtggtgct
     3181 ggccgtgttc ctcagcgagc aggatgaggt gctactgatc caggaggcca agagggagtg
     3241 ccgggggtcg tggtacctgc ctgcggggag aatggagcca ggggagacca tcgtggaggc
     3301 gctgcagcgg gaggtgaagg aggaggcggg gctgcactgt gagcccgaga cactgctgtc
     3361 cgtggaggag cggggcccct cctgggtccg cttcgtgttc ctcgctcgcc ccacaggtgg
     3421 aattctcaag acttccaagg aggccgatgc ggagtccctg caggctgcct ggtacccacg
     3481 gacctccctg cccactccgc tgcgagccca tgacatcctg cacctggttg aactagccgc
     3541 ccagtatcgc cagcaagcca ggcaccctct cattctgccc caagagctac cctgtgatct
     3601 ggtctgccag cggctcgtgg ctacctttac cagcgcccag acagtgtggg tgttagtggg
     3661 cacagtgggg atgcctcact tgcctgtcac tgcctgtggc ctcgacccta tggagcagag
     3721 gggtggcatg aagatggccg tcctgcggct gctgcaggag tgtctgaccc tgcaccactt
     3781 ggtggtggag atcaaggggt tgcttggact gcagcacctg ggccgagatc acagtgatgg
     3841 catctgtttg aatgtgctgg tgaccgtggc ttttcggagc ccagggatcc aggatgaacc
     3901 cccaaaagtt cggggtgaga acttctcttg gtggaaggtg atggaggaag acctgcaaag
     3961 ccagctcctc cagcggcttc agggatcctc tgttgtccca gtgaacagat acccaacttt
     4021 cttgtacaaa gtggtacgcg atgatgacta caaagacgat gacgacaagt gatcgcgtgg
     4081 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     4141 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     4201 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     4261 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     4321 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     4381 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     4441 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     4501 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     4561 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     4621 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     4681 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     4741 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     4801 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     4861 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     4921 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     4981 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     5041 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     5101 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     5161 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     5221 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     5281 tttgttagac gctagcggat ccataacttc gtatagtata cattatacga agttatgcca
     5341 ccatgtctag gctggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     5401 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     5461 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     5521 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     5581 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     5641 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     5701 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     5761 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     5821 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     5881 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     5941 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     6001 tcccagccga tgcccttgac gattttgacc ttgacatgct ccccgggtaa ataacttcgt
     6061 atagtataca ttatacgaag ttatggatcc gcggccgcaa attccgcccc tctccctccc
     6121 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     6181 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     6241 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     6301 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     6361 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     6421 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     6481 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     6541 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6601 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6661 tttgaaaaac acgataatac catggccacc gagtacaagc ccacggtgcg cctcgccacc
     6721 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6781 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6841 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6901 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6961 tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7021 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7081 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7141 cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7201 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7261 atgacccgca agcccggtgc ctgagttcgc gtctggaaca atcaacctct ggattacaaa
     7321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7681 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     7741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7801 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7861 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     7921 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7981 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     8041 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     8101 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8161 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8221 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8281 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     8341 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     8401 cttgacattg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     8461 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8521 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8581 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8641 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     8701 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8761 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8821 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8881 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8941 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9001 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9061 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9121 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     9181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9301 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     9421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9481 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa aagcctgaac
     9541 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     9601 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     9661 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     9721 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttaattcagc
     9781 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     9841 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     9901 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     9961 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    10021 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    10081 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    10141 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    10201 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    10261 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
    10321 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    10381 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    10441 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    10501 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgtg
    10561 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    10621 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    10681 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10801 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    10861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    10981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    11041 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    11101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    11161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    11221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    11281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    11341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    11401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    11461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    11521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    11581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    11641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    11701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    11821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    11881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    12301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    12361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    13021 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13141 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    13201 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    13261 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtac
//
