LOCUS       sequence-423138-       13367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for DOX-inducible NUDT17 gene expression with 
            C-terminal HA tag. Gateway expression system. Selection by 
            puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13367)
  TITLE     Chromosome 8p-engineering reveals increased metastasis targetable by
            patient-specific synthetic lethality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ-NUDT17-C-HA-Puro
FEATURES             Location/Qualifiers
     source          1..13367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    674..799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1296..1529
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1714..1758
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2056..2173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2238..2612)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2609..2618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2622..2640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2632..2679)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2715..2733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2750..2768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2786..2804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2822..2840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2857..2875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2893..2911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2923..2961
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2958..2982
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2995..3019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4012..4038
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4047..4071)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4077..4100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4110..5321
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5158..5177
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5334..5367)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             5374..6081
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(6082..6115)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6138..6710
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6305..6322)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6532..6551
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6713..7315
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7212..7232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7331..7919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7384..7404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7802..7813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8126..8359
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8460..8477)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8466..8690
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8736..9164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8823..8842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9033..9054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9173..9193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9178..9507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9358..9493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9420..9439
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10715..10836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10885..10901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10885..10901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10898..10920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10909..10925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10933..10939,10940..10957,10958..10963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10978..10999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11116..11133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11287..11875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11367..11386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12046..12837,12838..12906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12669..12688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12907..13011)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13059..13193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             join(13361..13367,1..627)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
       61 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
      121 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
      181 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
      241 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
      301 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
      361 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
      421 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      601 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      661 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      721 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      781 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      841 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
      901 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
      961 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1021 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1081 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1141 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1201 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1261 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1321 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1381 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1441 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1501 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1561 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1621 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1681 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1741 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1801 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     1861 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     1921 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     1981 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2041 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2101 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2161 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg
     2221 cccgctctag tccggaatca gtcctgctcc tcggccacga agtgcacgca gttgccggcc
     2281 gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc
     2341 ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc
     2401 aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg
     2461 ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc
     2521 cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg
     2581 agcaccggaa cggcactggt caacttggcc atggtggccc tcctatagtg agtcgtatta
     2641 tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgcag gtccgaggtt
     2701 ctagacgagt ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt
     2761 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2821 ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta
     2881 tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     2941 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcaccggt ggggacaagt
     3001 ttgtacaaaa aagcaggctc catggccgag gtgagagtgc agctgctgct gagcagaaga
     3061 cccgagtccg tgagttttgc tagaagcgtg tgtggtctgc tgggcgccgg cccgggactg
     3121 ggcacctggc ccatccactg cagcctgaag agaggcagac tggtgctgag ctcaagaccc
     3181 tttccgggcg ctagcgctcg cctgccgctg cagagacccc ctttttgtcc ttttgccgcg
     3241 ttagaggaac gtcctagagt gcccggcgcg gagctcccga ccgatcgcgg agtggacctg
     3301 ggcgtggccg tgatcctgca gagcagcgac aagaccgtgc tgctgacaag aagagcgcga
     3361 acactgagcg tgagccccaa tctgtgggtg ccccccggcg gccacgtaga actggaggag
     3421 gaattactgg acggcggcct gagagaactg tgggaagaga gcggcctgca cctgccccaa
     3481 gggcagttca gctgggtgcc cctgggcctg tgggagagcg cctacccccc tagactgagc
     3541 tggggcctgc ccaagtacca ccacatcgtg ctgtacctgc tggtgatcag ccaagagtct
     3601 cagcagcagc tgcaagctag aattcagccc aaccccaacg aagtgagcgc cctaatgtgg
     3661 ctaacccccg atgtggccgc cgccgtggca gcagccgaag acggcactga gacccccggc
     3721 ctgcttcccc aagacctgcc cccctcagta ctggccgtgg aactggaaga ggacggtaga
     3781 gctagacccc tggtgctgca catgagcacc ctgctgagaa tgatccccac catggccgag
     3841 gacaaggaga gagtgagcac cggcaccaag ttcgccctga agttgtggtt acaacacctc
     3901 ggcagaacac cccctccctg caaaagcgcc gcatatctgg accccggccc cgccaaggag
     3961 gagtggaaca tggaccccct gccccccaac caaggcagcg gcaagctagg ttacccatac
     4021 gatgttccag attacgcttg aaattaaccc agctttcttg tacaaagtgg tcccctgact
     4081 acaaagacga tgacgacaag tgaacgcgtg gcctccgcgc cgggttttgg cgcctcccgc
     4141 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     4201 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     4261 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     4321 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     4381 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     4441 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     4501 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
     4561 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
     4621 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
     4681 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     4741 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     4801 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     4861 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
     4921 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     4981 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     5041 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     5101 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     5161 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     5221 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     5281 tgtccgctaa attctggccg tttttggctt ttttgttaga cgctagcgga tccataactt
     5341 cgtatagtat acattatacg aagttatgcc accatgtcta ggctggacaa gagcaaagtc
     5401 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     5461 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     5521 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     5581 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     5641 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     5701 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     5761 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     5821 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     5881 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     5941 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     6001 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgattttgac
     6061 cttgacatgc tccccgggta aataacttcg tatagtatac attatacgaa gttatggatc
     6121 cgcggccgca aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     6181 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     6241 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     6301 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     6361 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     6421 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     6481 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     6541 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     6601 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     6661 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggccac
     6721 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     6781 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     6841 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6901 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6961 cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg gccgagttga gcggttcccg
     7021 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7081 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7141 cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg gtgcccgcct tcctggagac
     7201 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7261 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagttcg
     7321 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7741 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7921 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     7981 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8041 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8101 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     8161 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8221 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8281 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8341 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8401 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc gctgatcagc
     8461 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     8521 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     8581 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     8641 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     8701 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     8761 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8821 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8881 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8941 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9001 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9061 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9121 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     9181 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9241 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9301 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9361 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9421 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     9481 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     9541 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     9601 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     9661 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     9721 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     9781 gaagtgcttg acattgggga attaattcag cgagagcctg acctattgca tctcccgccg
     9841 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     9901 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     9961 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
    10021 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
    10081 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
    10141 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
    10201 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
    10261 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
    10321 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
    10381 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
    10441 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
    10501 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
    10561 cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt
    10621 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10681 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    10741 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10801 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    10861 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    10921 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    10981 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11041 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11461 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11701 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    12841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    12901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    12961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13021 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    13081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    13141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    13201 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    13261 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    13321 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagg
//
