LOCUS       sequence-423159-       12938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  His tagged human FASN cloned in pcDNA3.1 vector for expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12938)
  TITLE     His tagged human FASN cloned in pcDNA3.1 vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1_FASN
FEATURES             Location/Qualifiers
     source          1..12938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     611..630
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(751..1080)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      765..900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(819..838)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1065..1085
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1094..1522)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1204..1225)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1416..1435
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1568..1792
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1781..1798
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1818..1835)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9445..9464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9446..9464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9509..9712)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9538..9558)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9713..10092
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10264..10283
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10358..10462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10463..10531,10532..11323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10681..10700)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11494..12082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11983..12002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12236..12253
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12370..12391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12406..12411,12412..12429,12430..12436)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12444..12460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12449..12471
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12468..12484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12468..12484
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    12533..12654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12544..12563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12598..12617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(12828..12938,1..684))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
       61 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
      121 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
      181 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
      241 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
      301 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
      361 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
      421 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
      481 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
      541 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
      601 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
      661 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatcc
      721 gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc
      781 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
      841 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
      901 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
      961 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     1021 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     1081 attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1141 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1201 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1261 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1321 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1381 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1441 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1501 aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt tctttccgcc
     1561 tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc
     1621 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
     1681 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
     1741 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
     1801 gatcagcggg tttatcaatg gtgatggtga tgatggggcc ctctagactc gaggccctcc
     1861 cgcacgctca cgcgtggctc agccagggag ctgtggatga tgctgatgat ggactccagg
     1921 ccgctgccct ccagcagcgt gcggtggtca ccctcgatga cgtggacgga tactttcccg
     1981 tcgcatacct gggagaggtt gtagtccgcg cccaggtcct cgccgtaggc gccacccgtc
     2041 ttggcgcgca gtagcatcac gttgccatgg tacttggcct tgggtgtgta ctgctcagcg
     2101 gcacgcagct tgtagtagaa ggaccgggcc gcaaagctca gctcctggcg gtccaggccc
     2161 tggtggctct tgatgatcag gtccacggcg gctgccacac gctcctctag gcccttcagc
     2221 ggcagcagcg cctccagcac cctgttgtgc tccatgtccg tgaactgctg cacgaagaag
     2281 catatggcct ccgtctcagc ctcagcctca cagcctgggg tcagctttgc ccggtagctc
     2341 tgggtgtagg ccagtacgta ggtgggcgag ccgtcgaaca ggaagaggct gttgtgggtg
     2401 ggggctgggc tctgctgggc ctgcagctgg gagcacattt caaaggccac gcaggccccg
     2461 taggagtagc cggccacgcg gtaggggccc tcgggctgca cctgcctgat gcagtcgatg
     2521 tagtaggcag ccaggctgtg gatgctgtca aggggcgcag ctcgggtgca ctgcaggcca
     2581 taggtgggga tgctgagccg ggaggccagg ctgtggaaca cggtggtgga gccctcgatt
     2641 gggtgcacca ggaacagggg ccgctccgag ctctgcacgg agttgagccg catcagggtg
     2701 gggccctccg ggttcaccag cagggagcgc aggttcagct gagtctgctg ctgggccaga
     2761 ccatcctcct tgggcgtggg gcatgccagc tcgctggcct catccgcctt tgaggacagc
     2821 tcctgcagtt tccggagcgt gagttgccgc acctcgcgca cggacagcac caggttgagc
     2881 tcacgctcca gcgtctggcg cacctccacg ctcatgagcg agtccaggcc caggtccgcc
     2941 agtgagctgt ccaggttgac agcagccaag tcgcggatgc ccaggatgtg tgccacggcc
     3001 tccaccaggt cccgctggct gtccctgtcc ctataggccg cagccttctc agccagcaca
     3061 aagctgctca ggaccatgtg gggctggttc aggaagaggt ccagcacctc caggcaggac
     3121 gccatgcgct ggggcagcgt gccactgacg atcgtgtcgt tggtgctcat cgtctccacc
     3181 aaaatgccca cgtcgccgat ggcgccccac tgcacggcca ggcctgggag gccttcgtgc
     3241 cggcgtttct cacagatacg ctccatggcg gaattggcaa agccgtagtt gctctgtccc
     3301 gcattgccac gcccgcagct cacagaggag aagaccacaa agtagtccag ctcagggcac
     3361 gcctctcggg tcaccctgtc caggttcagg gtgccgctgt acttgggctt gcagacgtcc
     3421 tggaagaact ctggggtctg gttctccagc aagccatctc tcaagaccac ggccaggttg
     3481 aagacgccgc ccacgggccc aagctgcgcc gcctcggcaa tgaggccccg ggccccctcc
     3541 agtgagctga tgttgctggt ggacacctgc acctgtacgc cctggcgcct ccaccggcgg
     3601 acctgcttgg cctggtagcc tgtccggatc ccggagcgag aagtcaacac gagcttctgc
     3661 accccacgct gtatcagcca ctgcgccaac tccaggccga agccacccag accaccagcg
     3721 atgatgtagc tcttgtgggc cgggcagaag gtcttggaga tggccgacat cagcttgggt
     3781 ttggccccct tcagcactgc ctccggctcc tccgcaagca cctgcacgac gactttgcca
     3841 atgtgcttcc cttgggccat gtagcggaag gcgtcctcca cctgggcccc atggaacacc
     3901 gtgcacttga ggggccgtac caccccatcc cggatgccgg cctgcacaag cgcccacacc
     3961 tcccgccagt cagcactgct ctcgttgaag aacgcatcca gtaggacccc gtggaatgtc
     4021 acgttcttca ggaagatagc catgccgagc gggtggttct gagaaaggtc gaatttgcca
     4081 atttccagga agcgaccgtg cgtagccaag cacctcacgc tggcctgcag cttctcttcc
     4141 gccaaggagt tcaagaccag gtcaacgccc ttcccgcccg tgtgccacag cacatgctgc
     4201 tcgaaggatg tgtcccggga gttggcgaag ctggtgctgt cgagctgggg gaacctggcc
     4261 tggaggtacg cccgcttctc agccgacccc acggtggtga agacgcggca gcccagactg
     4321 agggcgatgg cgatggcggc ctggcccacg ccgcccgagc ccgagtggat gagcagcgtc
     4381 tccccggggc gcacccgccc acgcaccacc agcgcgtagt aggccgtgct gtagacgaca
     4441 ggcaccgagg ccgcctcctc cagcgtccag ttggaaggca catcccagag gaagtccggt
     4501 gacagcagga cagaggtggc caggcccttg gcaggcacca gtcccatcac acgcttgccg
     4561 ctggcgtctc ggcccgagaa ctccatacct agcaggctgt cctgggaggt ccacttccct
     4621 gggatggcat caggggacag cttgccagtg gccagcatga tgtcgcggaa gttgagggag
     4681 gcgtagtaga ccgtgcagag ctgggcgcca gggcaggtgg gctgggcatg gcgcagcgag
     4741 gagcagaccc agcggatgga ggacaggtcc ccccgggtga gggtgctcac aaaggcatgt
     4801 gccgtcggct cctcaggctt gtcctcctcc agcaggaagt ggcggaaagc cccccaggcc
     4861 ccgtcgcggt agacgttcat caccaggtct ccctgcaaca ccttctgcag ttctgcggag
     4921 cccgggtcca cctccgggac gtgggaggtg ctgctgaggt tggagagcag cacacaccgg
     4981 aggcggttcc cgccgggctc tcggcggaga cagttcacca agcccaccac gcccgaggtg
     5041 gcacagttga tggccttcag ccacacaggc cgggaagagt cttcgtcagc caggatgccc
     5101 ttcagagact ccacccagcg gaagctggta tcgtccaccg gcaggaagat ggggctgtcc
     5161 tgcggggtgg gccggcggca caggaagagc gtggagccgt agaaggactt cttcaggccc
     5221 accaggcgca gcgacaccct ggagaagagg ctctcccacg cgtcctggct caggatgccc
     5281 tggccatact gcggctcagt ggaggtgagg aaggccacga tgtccccgag ggggtgcccc
     5341 cggagcagtg tgtgcaggag cagaaagccc ccttctctca gggcagccac catgttgctg
     5401 agagctgagg ccgggtcccc gagggcagcc acagcacagt tgcacaccag gaggtcggcg
     5461 ctgcccaggg cgctgggggc agggtctgcg ggatcccact ggccctgggc aacgtcgtgc
     5521 tgctgcagct cggcctgggc agcctccagg gcctgggggt ggcggtcggt ggccgtgtag
     5581 ctcagctgca gcaggggatg ggggctgagc aggcctggga tgcgggaata caggtgaccg
     5641 tggccagcca gcacctccac caccttcatc ttcaggctgg gcatgttctc cacggcagtg
     5701 tccaggcagg ccttgagtgc cggggagtcc aggaggccgc tgagcagagg gtcctctggc
     5761 agcttgggcc tctcctgggc cagcacctgc gccagctcca gctgcaggtt cccgttgagc
     5821 tgaagcctgc aggcagccga caacagccgg ggcagttcct gctgtgaggg gtcccggggg
     5881 atctgggccc catccagtcc gggcaccacc atcttcagcc cctgctgggt caccttggtc
     5941 tgcagtgcct gcaccagccc cttgcacagt tgcagctcct cctgcagggc agcgcgctca
     6001 gacaggcacc cctcctccgt gtggggagtg aagcaaaact tctccaggat gggcacctgc
     6061 tgctcctgct gccgccgcgg ggccgactca gtgtggagcc cggagatgtg gacgcctccg
     6121 gccactgtga ccctcagcca cctgctcacc accacgtcag ccacttgggc cttgtcctgc
     6181 agtgtgtaca gcttctgcct gtgggtggca gggtcgatgt ggatggcggt gacacgggtg
     6241 ggcaggtaca ggccgtgctt ggccgagccc aggatggaca tctgcagcat ggtgtccatg
     6301 aagctcaccc agttatcctt ccacagcagc ctccccgagt caccttccag gctggcctcc
     6361 aggatgccct ggaaatgagg gccgtagtcg tagccacgca gacgcagctc cttgtaaact
     6421 tcagcctggg ccaggaagag gggctccgtg gggttggggg tggggctttc cgggtggtcg
     6481 aagagcctgg ggtcagggtc atcccactgg tacaccttcc cactcactac caggttgccg
     6541 ttctctgaca cctcgaaggc acgggaggcc tccaggagcc gtacctccag ggacactgtc
     6601 ccagtcttgg gcaggatggt ggcctggtgc agcaccacat cctcaaacac cacaggcagc
     6661 tgctcgacgc ccaggcccag ggcgcgggcc agcgtcttcc acactatgct caggtagcca
     6721 gtggcgggga agaggacgcg accgtcgagg gtgtggtcca ccaggtagtg gtcaggagac
     6781 tcggagctgg tgtcgatgtt gtagatggcg gctgaggggg aacctgaacc gttggggaag
     6841 tcctcggcgg ccggcacgtc ccaggccagg ctgtggtccc acttgatgag tggggagatg
     6901 aggggagttc ctcggggagc tgggaactcc acaggtggga acaaggcatt ggggttggcg
     6961 tcgatgcctg agaggtgcag cctgccgatg ccggccagga agaactccag gttgtccctg
     7021 tgatccttct tcatcagggg gatgatggtg cagctcggct tcaggccacg cttcaggaca
     7081 gcctgcagca gggcgtgggg cgcgatctcc agcaccaccg cgtgctcagg cacgtgccac
     7141 agggcctcct ggaacagcac agggctcacc aggttgttga cattgtactc ggcggaggac
     7201 gtgcgtgcca ggctgctgtg ccactgggcc tcggggatag aggtgctgag ccagcgggct
     7261 gaacgtggct tcggctcccg gatcaccttc ttgagctcct gcagcagtgg gggtgcgatg
     7321 gcctccatga agtaggagtg gaaggccata ccgccggtcc gcacctcctt ggcaaacaca
     7381 ccctccttcc tcagctgctc cacgaactca aacaccgggg cctgaggtcc cgagatggtg
     7441 actgtgtcct tggagttgtg gcaggcgggc accacgcccg gggggcagcg ctgtttacac
     7501 tcctcccagg acaagcccac ggctgccatg gcgcccggcg ggagatgggc ttctttgatg
     7561 cactgtcccc tccagtaggc agcgaggacg gcctcctcct gggacaggca gccgtcggcg
     7621 tagccacagg ccacctcccc cagggagtgg ccgacgatgc catctggcct cagccccatg
     7681 cagctcagca ggtctatgag gcctatctgg atggcagtca ggctcacaaa cgaatggacg
     7741 atgtcatcaa aggtgctctc gtctgtgctc agcagcagct gtgacacctt caggccgaat
     7801 ggcttcacag cctcatcgga gcgtaggatg gaatctcgga agcggtccag gcgcatgagg
     7861 ctcagcccca tcccgcgcca ctgtgtgccc atcccagagc agatgaacca gagcgggcgc
     7921 tcgccagcgg gcacctgctg cacctctggg ccaccgcgct caccacccag cacagcgtag
     7981 ccacggaagg gcatggcggt ggcggggaca gccgcgatgt cgttcagcat gctcaggaaa
     8041 gccaggtcct ggctgtgccg gaggccctgc tccagcagct tctgcacggc ctcaggggtg
     8101 cgtccgctgg cccgcagcag acggggcagg gtggcatgtg gggcgggtgc ggggggcggc
     8161 tgcgtgttgg gcctcaggat gatgtgcacg ttggagcccc cgaagccaaa ggagttgatg
     8221 cccacgttgc cgccacggac gggcaggggc tggtccacca cctgcagccg cccatccaac
     8281 agcgctggga tctcagggtt ggggctatgg aagtgcaggt tgggggccca gagcccgtgc
     8341 tccagggaca gcagcacctt ggccagggct gccagccccg aggctggctc cgggtgcccc
     8401 atgttggact tggtggagcc gatgagcagc ggctcctggc gggtggcgca cagggctcgg
     8461 gtgatgccat tcagctcctg ggggtcgccc accttggtgc ctgtgccgtg ggcttcgatg
     8521 tattcaaatg actcaggggc cactccggcc gactggtaca acgagcggat gagctgctcc
     8581 tggatatccc ctgaggggaa ggtcacgcct tgctccttga agccatctgt attggtgccg
     8641 gcgttcagga tggtggcgta cacccgccgg gccagggact tcttggtcag caggacggcc
     8701 accacaccct ccgagcggca gtacccattc cccgctgtgt cgaaggcctt gcaggtgccc
     8761 tcggggctga gcatccccag cctcaagaac tgcacggagg tgttgggctt cagcaggaca
     8821 ttgatgcccc ccacgatggc ggcagggcac tgcccgctgt ggatggcctg gtaggcgttc
     8881 tgcagggcca tcaggctgga ggagcaggct gtgtccagtg cgatgctggg ccctctgaag
     8941 tcgaagaaga aggagagccg gttggccatc atcgctcgct ggcagcccac catgctgtag
     9001 cccacgagtg tctcggggtc tcggctcagg gcctccgagg tctcagagcc gctcacgccc
     9061 acccagacgc cagtgtgtgt tcctcggagt gaatctgggt tgatgcctcc gtccacgatg
     9121 gcttcatagg tgacttccag cagcagccgc agctgagggt ccatcgtgtg tgcctgcttg
     9181 gggtggactc cgaagaagga ggcatcaaac ctagacaggt ccttcagctt gccggaccgc
     9241 cggggcaggc cgtagagccc cgccttccag cgacggtcat cgtccgtgac catgtccaca
     9301 ccgccgatga ggttgtccca gaactcctgc aagttctccg actctggcag cttcccggac
     9361 atgccggcaa tcaccacctc ctccatgaat tcggatccga gctcggtacc aagcttaagt
     9421 ttaaacgcta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
     9481 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
     9541 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     9601 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     9661 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     9721 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     9781 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     9841 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     9901 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     9961 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
    10021 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
    10081 ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa
    10141 ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt
    10201 tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact
    10261 atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga
    10321 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    10381 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    10441 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    10501 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    10561 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    10621 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    10681 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    10741 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    10801 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    10861 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    10921 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    10981 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    11041 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    11101 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    11161 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    11221 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    11281 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    11341 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    11401 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    11461 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    11521 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    11581 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    11641 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    11701 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    11761 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    11821 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    11881 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    11941 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    12001 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    12061 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    12121 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    12181 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    12241 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    12301 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    12361 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    12421 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    12481 tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat gataagatac
    12541 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
    12601 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttggggtg
    12661 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
    12721 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
    12781 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
    12841 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
    12901 aggaagcggt cagcccattc gccgccaagc tcttcagc
//
