LOCUS       sequence-423160-        9150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for CD63-positive exosome secretion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Sung BH, von Lersner A, Guerrero J, Krystofiak ES, Inman D, 
            Pelletier R, Zijlstra A, Ponik SM, Weaver AM
  TITLE     A live cell reporter of exosome secretion and uptake reveals 
            pathfinding behavior of migrating cells.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2092. doi: 10.1038/s41467-020-15747-2.
  PUBMED    32350252
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2092. doi"; pages: " 
            10.1038/s41467-020-15747-2"
COMMENT     Sequence Label: pLenti-pHluorin_M153R-CD63
FEATURES             Location/Qualifiers
     source          1..9150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        69..372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..576
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     527..547
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     573..597
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    681..705
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             855..1565
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIRADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             855..1565
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIRADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(882..910)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1505..1525
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(2170..2194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2225..2813
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2278..2298)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2696..2707)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2864..2884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2869..3198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3049..3184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3246..3293
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3312..3710
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3797..4030
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4102..4223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4139..4158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4193..4212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4263..4398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4418..4437)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4419..4437)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4447..4464)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4447..4463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4456..4478)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4605..5060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4692..4711)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4902..4923
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5086..5190
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5191..5259,5260..6051)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5409..5428)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6222..6810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6711..6730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6964..6981
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7098..7119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7134..7139,7140..7157,7158..7164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7172..7188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7177..7199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7196..7212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7196..7212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7231..7251
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7233..7251
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7279..7505
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7506..7686
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7733..7858
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8351..8584
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8769..8813
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9080..9150,1..46)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
       61 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      121 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      181 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      241 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      301 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      361 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      421 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      481 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      541 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      601 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gtccagtgtg
      661 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctccgcg gccgccccct
      721 tcaccatggc ggtggaagga ggaatgaaat gtgtgaagtt cttgctctac gtcctcctgc
      781 tggccttttg cgcctgtgca gtgggactga ttgccgtggg tgtcggggca cagcttgtcc
      841 tgagtcagac cataagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      901 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      961 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1021 ggccaacact tgtcactact ctcacttatg gtgttcaatg cttttcaaga tacccagatc
     1081 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1141 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1201 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1261 ttggacacaa attggaatac aactataacg atcaccaggt gtacatccgg gcagacaaac
     1321 aaaagaatgg aatcaaagct aacttcaaaa ttagacacaa cattgaagat ggaggcgttc
     1381 aactagcaga ccattatcaa caaaatactc caattggcga tgggcccgtc cttttaccag
     1441 acaaccatta cctgtttaca acttctactc tttcgaaaga tcccaacgaa aagagagacc
     1501 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1561 acaaaatcca gggggctacc cctggctctc tgttgccagt ggtcatcatc gcagtgggtg
     1621 tcttcctctt cctggtggct tttgtgggct gctgcggggc ctgcaaggag aactattgtc
     1681 ttatgatcac gtttgccatc tttctgtctc ttatcatgtt ggtggaggtg gccgcagcca
     1741 ttgctggcta tgtgtttaga gataaggtga tgtcagagtt taataacaac ttccggcagc
     1801 agatggagaa ttacccgaaa aataaccaca ctgcttcgat cctggacagg atgcaggcag
     1861 attttaagtg ctgtggggct gctaactaca cagattggga gaaaatccct tccatgtcga
     1921 agaaccgagt ccccgactcc tgctgcatta atgttactgt gggctgtggg attaatttca
     1981 acgagaaggc gatccataag gagggctgtg tggagaagat tgggggctgg ctgaggaaaa
     2041 atgtgctggt ggtagctgca gcagcccttg gaattgcttt tgtcgaggtt ttgggaattg
     2101 tctttgcctg ctgcctcgtg aagagtatca gaagtggcta cgaggtgatg tagaagggtg
     2161 ggcgcgccga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     2221 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2281 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2341 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2401 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2461 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2521 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2581 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     2641 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2701 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2761 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     2821 tctgcagata tccggttagt aatgagtttg gaattaattc tgtggaatgt gtgtcagtta
     2881 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2941 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3001 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3121 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3181 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     3241 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     3301 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     3361 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     3421 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     3481 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     3541 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     3601 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     3661 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg
     3721 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3781 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     3841 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3901 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3961 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4021 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4081 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4141 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4201 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     4261 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4321 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4381 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     4441 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4501 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4561 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     4621 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4681 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4741 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4801 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4861 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4921 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4981 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5041 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5101 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5161 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5221 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5281 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5341 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5401 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5461 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5521 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5581 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5641 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5701 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5761 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5821 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5881 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5941 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6001 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6061 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6121 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6181 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6241 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6301 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6361 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6421 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6481 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6541 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6601 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6661 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6721 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6781 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6841 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6901 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6961 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7021 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7081 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7141 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7201 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     7261 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7321 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7381 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7441 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7501 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7561 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7621 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7681 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     7741 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7801 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     7861 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     7921 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     7981 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8041 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8101 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8161 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     8221 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8281 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8341 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8401 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8461 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8521 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8581 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8641 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8701 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8761 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     8821 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     8881 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     8941 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     9001 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     9061 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9121 aaagaatagt agacataata gcaacagaca
//
