LOCUS       sequence-423164-        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of PE6c prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, 
            McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, 
            Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR
  TITLE     Phage-assisted evolution and protein engineering yield compact, 
            efficient prime editors.
  JOURNAL   Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 
            10.1016/j.cell.2023.07.039.
  PUBMED    37657419
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.07"; journalName: "Cell"; date: "2023-08-31- 
            31"; volume: "186"; issue: "18"; pages: "3983-4002.e26"
COMMENT     Sequence Label: pCMV-PE6c
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(200..991,992..1060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     823..842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1061..1165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1244..1263)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1435..1814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1815..2018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1969..1989
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2060..2079
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2060..2078
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2128..2148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2149..6249
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6307..6327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7912..7932
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7942..7968
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7977..7994
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8017..8034)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8023..8247
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8318..8334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8318..8334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8331..8353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8342..8358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8366..8372,8373..8390,8391..8396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8411..8432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8549..8566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8720..9279,1..29))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8800..8819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
       61 acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      121 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      181 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      241 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      301 agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc tcaccggctc
      361 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
      421 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
      481 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
      541 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
      601 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
      661 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
      721 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
      781 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
      841 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
      901 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
      961 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1021 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1081 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1141 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     1201 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     1261 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     1321 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     1381 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     1441 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     1501 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     1561 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     1621 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     1681 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     1741 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     1801 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     1861 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     1921 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     1981 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
     2041 gctagagatc cgcggccgct aatacgactc actataggga gagccgccac catgaaacgg
     2101 acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtcga caagaagtac
     2161 agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     2221 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     2281 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     2341 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     2401 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     2461 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     2521 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     2581 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     2641 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     2701 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     2761 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagaaagct ggaaaatctg
     2821 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     2881 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     2941 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     3001 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     3061 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     3121 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     3181 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     3241 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     3301 accgaggaac tgctcgtgaa gctgaagaga gaggacctgc tgcggaagca gcggaccttc
     3361 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     3421 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     3481 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     3541 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     3601 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     3661 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     3721 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     3781 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     3841 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     3901 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     3961 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     4021 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     4081 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     4141 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     4201 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     4261 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     4321 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     4381 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     4441 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     4501 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     4561 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     4621 caggaactgg acatcaaccg gctgtccgac tacgatgtgg acgctatcgt gcctcagagc
     4681 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     4741 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     4801 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     4861 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     4921 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     4981 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     5041 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     5101 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     5161 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     5221 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     5281 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     5341 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     5401 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     5461 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     5521 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     5581 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     5641 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     5701 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     5761 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     5821 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     5881 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     5941 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     6001 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     6061 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     6121 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     6181 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     6241 ggaggtgact ccggcggaag ctctggtggc agcaagcgga ccgccgacgg ctctgaattc
     6301 gagagcccta agaagaaaag aaaggtgagc ggaggctcta gcggcggaag catcagcagc
     6361 tctaagcaca ccctgagcca gatgaacaag gtgagcaaca tcgtgaagga acccgagctg
     6421 cccgacatct acaaggaatt taaggacatc accgccgaca ccaataccga gaagctgcct
     6481 aagcctatta agggcctgga atttgaagtg gaactgacac aggagaacta cagactgcct
     6541 atccggaact atcctctgac tccagtcaag atgcaggcca tgaacgacga gatcaatcaa
     6601 ggcctgaaag gcggcatcat cagagagagc aaggccatca acgcctgccc tgttatattc
     6661 gtgcccagaa aggaaggcac actgcgcatg gtggtcgact acaggcccct gaacaagtac
     6721 gtgaagccca acgtctaccc cctgccactg attgaacaac tgctggccaa gatccagggc
     6781 agcaccattt tcaccaagct ggacctgaaa agcgcctacc accagatcag agtgcgaaag
     6841 ggagatgagc acaagctggc cttccggtgc cccagaggag tcttcgagta cctggtgatg
     6901 ccttacggca tcaagacagc ccctgcccac tttcagtact tcatcaacac aatcctgggc
     6961 gaggccaagg aaagccacgt ggtgtgctac atggacgaca tcctgatcca ttccaagtcc
     7021 gagtccgaac acgtgaaaca tgtgaaggac gtgctgcaaa agctgaagaa cgctaatctg
     7081 atcatcaacc aggccaaatg cgagtttcac cagagccaag tgaagttcct gggctaccac
     7141 atcagcgaga agggcttaac cccatgtcag gagaacatcg acaaggtgct gcagtggaaa
     7201 cagcctaaaa accagaagga actgagacag ttcctgggcc aggtgaacta cctgagaaaa
     7261 ttcatcccca aaaccagcca gttgacccac cctctgaaca aactcctgaa aaaggatgtc
     7321 agatggaaat ggacccctac ccagacccag gctatcgaga atatcaagca atgtctggtg
     7381 tctcctcctg tgctgaggca cttcgacttc agcaagaaga tcctgcttga gacagacgtt
     7441 tctgatgtgg ccgtgggagc cgtgctgagc cagaagcatg atgatgataa gtactaccct
     7501 gtgggctatt acagcgctaa aatgagcaaa gcccagctga attattctgt gtccgacaag
     7561 gagatgctgg ctatcatcaa aagcctggag cactggcggc actacctgga atctacaatc
     7621 gagcccttca agatcctgac cgaccacaga aacctgattg gaagaatcac aaacgagagc
     7681 gaaccagaga acaagcggct ggccagatgg cagctgttcc tgcaggactt caacttcgag
     7741 atcaactaca gacctggctc tgcaaatcac atcgccgatg ccctgtctag aatcgtggac
     7801 gagactgagc ctatccctaa ggacaacgaa gataacagca tcaacttcgt gaaccagatc
     7861 agcatctctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagtc tcccaagaag
     7921 aagaggaaag tcggctctgg ccctgccgct aagagagtga agctggacta accggtcatc
     7981 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8041 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8101 ctgtcctttc ctaataaaat gagaaaattg catcgcattg tctgagtagg tgtcattcta
     8161 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8221 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     8281 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8341 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8401 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8461 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8521 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8581 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8641 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8701 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8761 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8821 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8881 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8941 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9061 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9121 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9241 ccaccgctgg tagcggtggt ttttttgttt gcaagcagc
//
