LOCUS       sequence-423192-        8366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5
            variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Zhang QE, Lindenberger J, Parsons R, Thakur B, Parks R, Park CS, 
            Huang X, Sammour S, Janowska K, Spence TN, Edwards RJ, Martin M, 
            Williams WB, Gobeil S, Montefiori DC, Korber B, Saunders KO, Haynes 
            BF, Haynes BF, Henderson R, Acharya P
  TITLE     SARS-CoV-2 Omicron XBB lineage spike structures, conformations, 
            antigenicity, and receptor recognition.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 12:2024.02.12.580004. doi: 
            10.1101/2024.02.12.580004.
  PUBMED    38405707
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 12:2024.02.12.580004. doi: 
            10.1101/2024.02.12.580004."
COMMENT     Sequence Label: p-alpha-H-S-GSAS/EG.5
FEATURES             Location/Qualifiers
     source          1..8366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             638..661
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             665..688
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             695..718
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             758..781
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(883..902)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    948..1003
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1002..1021)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1364..1380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1364..1380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1377..1399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1388..1404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1412..1418,1419..1436,1437..1442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1457..1478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1607..1624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1778..2366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1858..1877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2537..3328,3329..3397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3160..3179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3398..3502)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3533..3912
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4192..5208
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5132..5154
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gatgtccgag tgtgtgctgg gacagtctaa acgcgttgac ttctgcggca aaggctatca
       61 cctgatgagc ttcccccaga gcgcgccgca cggcgtggtg ttcctgcatg tgacatacgt
      121 gcctgcccaa gagaagaatt tcacaaccgc ccctgccatc tgccacgacg gcaaggccca
      181 cttcccaaga gagggcgttt tcgtttccaa tggcacacac tggttcgtga cacaaagaaa
      241 cttctacgaa ccccagatta tcaccaccga caacaccttc gtgagtggca attgtgacgt
      301 ggtcatcgga atcgtgaaca acacagtgta cgaccctctg caacctgagc tggactcttt
      361 taaggaagag ctggacaagt actttaaaaa ccacaccagc cccgatgtgg acctgggcga
      421 catcagtggc attaacgcca gcgtggtgaa catccaaaag gaaatcgaca gactgaacga
      481 ggtggccaag aacctgaacg agtccctgat cgacctgcag gagctcggca aatacgagca
      541 gggatccgga tacatccccg aggcccccag agatggccag gcctacgtgc ggaaggacgg
      601 cgagtgggta ctgctgagca cattcctggg cagatccctg gaggtgctgt tccagggccc
      661 aggccatcac caccatcacc accatcatag cgcctggtcc cacccccagt tcgagaaggg
      721 cggcggtagt ggagggggcg gatctggcgg ctcagcttgg agccaccccc agttcgaaaa
      781 gtgataatga ctcgagcgat aattcactcc tcaggtgcag gctgcctatc agaaggtggt
      841 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
      901 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      961 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     1021 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     1081 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     1141 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     1201 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     1261 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     1321 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     1381 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1441 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1501 ctttccagtc gggaaacctg tcgtgccagc ggatcgatcc gctgcattaa tgaatcggcc
     1561 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1621 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1681 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     3541 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     3601 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3661 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3721 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3781 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3841 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3901 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     3961 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     4021 gggggggggg gggggggggc cccggggggg gggggggggg gggggggggg gggggggggg
     4081 gggggggggg gggggggggg gggcggcccg gggggggcgc cccccaagtg ttcttttttg
     4141 tagggggggg gggggggggg gcccccaaaa aaaagagacg gggggcgggc gggagtcgct
     4201 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     4261 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     4321 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     4381 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     4441 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     4501 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     4561 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     4621 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     4681 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     4741 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     4801 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
     4861 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     4921 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     4981 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     5041 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     5101 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     5161 tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     5221 gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tgcggccgtc tcaggccgag
     5281 ttcggtaccg ccaccatgtt cgtgttcctg gtgctcctgc ctctggtgag cagccagtgc
     5341 gtgaacctga tcacccgaac ccagtcctac accaacagct ttacacgggg cgtgtactac
     5401 cctgacaagg tgttcagatc tagcgtcctg cacagcactc aggacctctt cctgccgttc
     5461 ttcagcaacg tgacatggtt ccacgccatc cacgtgagcg gcacaaacgg aaccaagcgg
     5521 tttgataacc ccgccctgcc attcaatgat ggagtttact tcgccagtac cgagaagagt
     5581 aacatcatcc ggggctggat cttcggcacc accctggata gcaaaacaca gagcctcctg
     5641 atcgtgaaca atgccacgaa cgtcgtgatc aaggtgtgcg agttccagtt ttgcaatgat
     5701 cctttcctgg acgtgtacca gaagaacaac aagagctgga tggaaagcga gttcagagtc
     5761 tacagcagcg ccaacaactg cacattcgag tacgtctctc agccttttct gatggacctt
     5821 gaggggaaag aaggcaactt caagaacctg agagaattcg tgttcaagaa catcgacggc
     5881 tacttcaaaa tctactccaa gcacacaccc atcaacctgg agcgggacct ccctcagggc
     5941 ttcagcgccc tggaacccct ggtcgacctg cccataggca tcaacataac gcggttccaa
     6001 accctgctgg ccctgcatag atcctacctg actcctgtgg acagcagcag cggatggacc
     6061 gccggagctg cagcctacta tgtgggctac ctgcaaccta gaaccttcct gctgaagtac
     6121 aacgagaacg gcacaatcac agacgccgtc gactgcgccc tggaccctct ctctgagaca
     6181 aagtgcaccc tgaagtcctt caccgtggaa aagggcatct accagaccag caacttccgg
     6241 gtgcagccta cagagagcat cgtgcgattt ccaaacatta ccaacctctg ccccttccac
     6301 gaggtgttta acgccacaac atttgcctcc gtttacgcct ggaatagaaa gagaatcagc
     6361 aattgtgtgg ccgactactc cgtgatctat aacttcgccc ctttcttcgc cttcaagtgc
     6421 tacggcgttt ccccaacaaa gctgaatgac ctgtgcttca ccaacgtgta cgccgactcc
     6481 ttcgtaatta gaggcaatga ggtgtcccag atcgcaccag gccagaccgg taacatcgct
     6541 gactacaact ataagctgcc tgatgatttt acaggctgcg tgatcgcctg gaactccaac
     6601 aagctggata gcaagcctag cggcaactac aactacctgt accggctgct gcgcaagtct
     6661 aagctgaaac ctttcgagag agacatctcc acagagatct accaggccgg taacaagcct
     6721 tgtaacgggg tggccggccc caactgttac tcccctctgc aaagctacgg cttccggcct
     6781 acctatggag tcggccacca gccataccgg gtggtcgtgc tgtccttcga gttactccac
     6841 gcccccgcca ccgtctgcgg tcctaagaag tccaccaatc tggttaagaa caaatgcgtg
     6901 aacttcaact tcaacggcct gaccgggacc ggcgtgctga ccgaaagcaa caaaaagttc
     6961 ctccccttcc agcagttcgg ccgtgatatc gctgacacca cagatgccgt cagagatcca
     7021 cagaccctgg aaatcctgga tattacaccc tgctccttcg gaggagtttc tgtgatcacc
     7081 cccgggacca ataccagcaa ccaggtggct gtgctgtacc aaggtgttaa ctgcaccgag
     7141 gttcctgtgg ccatccacgc cgatcagctg acacctactt ggagagtgta ctccactggc
     7201 tccaatgtgt tccagaccag ggccggatgt ctgatcggcg ccgagtacgt gaataacagt
     7261 tacgagtgcg acatccctat cggcgccggc atctgtgcca gctaccagac ccagacaaag
     7321 agccatgggt ctgcttcctc tgtagctagc cagagcatca tcgcctacac catgagcctg
     7381 ggcgcagaga acagcgtggc ctattccaac aactctatcg ccattcccac caactttaca
     7441 attagcgtca caacagagat cctgcccgtg agcatgacca agaccagcgt ggactgtaca
     7501 atgtacatct gtggcgacag cactgaatgc agcaacctgc tgctgcaata cggctccttt
     7561 tgcacccaac tgaagcgggc gctgaccgga atcgccgtgg aacaggacaa aaatacccag
     7621 gaggtgttcg cccaagtgaa gcagatctac aagaccccac ctatcaagta cttcggcggc
     7681 tttaacttta gccagattct ccctgatcct tctaagccta gcaagcggag ctttatcgag
     7741 gatctgctgt tcaacaaggt caccctggcc gatgccggct ttatcaaaca gtatggcgat
     7801 tgtctgggcg acatagccgc cagagatctg atctgcgccc agaaattcaa cggcctgaca
     7861 gttctcccac ctctgctgac cgacgagatg atcgctcagt acacctctgc cctgctggct
     7921 ggcaccatca catctgggtg gacatttggc gccggcgccg ccctgcagat cccctttgcc
     7981 atgcagatgg cctatagatt caacggaatc ggcgtgaccc agaacgtgct gtatgaaaac
     8041 cagaagctga tcgctaacca gttcaattct gccatcggca agatccagga ctccctctcc
     8101 tctaccgcca gcgccctggg caaactgcag gacgtggtga atcacaacgc ccaagccctg
     8161 aacaccctgg tgaagcagct cagcagcaag tttggcgcca tcagctctgt gctgaacgat
     8221 atcctgtcta gactggacaa ggtggaagcc gaagtccaga tcgatagact gatcacaggc
     8281 agactgcagt ccctgcaaac ctacgtgacc caacagctga tcagggccgc tgaaataaga
     8341 gccagcgcca atctcgccgc taccaa
//
