LOCUS       sequence-423193-       11104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human telomerase reverse transcriptase, the catalytic 
            subunit of telomerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Reilly CR, Myllymaki M, Redd R, Padmanaban S, Karunakaran D, Tesmer 
            V, Tsai FD, Gibson CJ, Rana HQ, Zhong L, Saber W, Spellman SR, Hu 
            ZH, Orr EH, Chen MM, De Vivo I, DeAngelo DJ, Cutler C, Antin JH, 
            Neuberg D, Garber JE, Nandakumar J, Agarwal S, Lindsley RC
  TITLE     The clinical and functional effects of TERT variants in 
            myelodysplastic syndrome.
  JOURNAL   Blood. 2021 Sep 9;138(10):898-911. doi: 10.1182/blood.2021011075.
  PUBMED    34019641
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1182/blood"; 
            journalName: "Blood"; date: "2021-09-9- 9"; volume: "138"; issue: 
            "10"; pages: "898-911"
COMMENT     Sequence Label: TERT - WT
FEATURES             Location/Qualifiers
     source          1..11104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        73..144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(145..213,214..1005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(363..382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1176..1764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1665..1684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1918..1935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2052..2073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2088..2093,2094..2111,2112..2118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2126..2142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2131..2153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2150..2166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2150..2166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2185..2205
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2187..2205
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2233..2459
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2460..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2687..2812
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3305..3538
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3723..3767
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3980..4097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4151..4465
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4159..4177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4195..4213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4230..4248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4266..4284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4302..4320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4337..4355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4438..4462
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4486..4510
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4516..7914
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     protein_bind    complement(7916..7940)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7958..7975
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        7985..8495
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8414..8432
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8505..9101
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8505..8524)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9001..9021
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9102..9155
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9156..9773,9774..9779,9780..9815,9816..9818,9819..9854,
                     9855..9857,9858..9893,9894..9902)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9942..9962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10360..10371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10576..10809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10881..11002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10918..10937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10972..10991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(11042..11104,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      241 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1801 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1861 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1921 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1981 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2041 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2101 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2161 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     2221 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     2281 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     2341 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     2401 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     2461 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2521 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2581 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2641 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     2701 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     2761 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     2821 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     2881 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     2941 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     3001 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     3061 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     3121 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     3181 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     3241 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3301 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3361 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     3421 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     3481 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     3541 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     3601 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     3661 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     3721 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     3781 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     3841 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     3901 cattatcgtt tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa
     3961 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     4021 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     4081 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcgaa
     4141 tcgatatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     4201 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     4261 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     4321 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     4381 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     4441 tcgtttagtg aaccgtcaga tcgcctggag aattggctag catcaacaag tttgtacaaa
     4501 aaagcaggct tcaccatgcc gcgcgctccc cgctgccgag ccgtgcgctc cctgctgcgc
     4561 agccactacc gcgaggtgct gccgctggcc acgttcgtgc ggcgcctggg gccccagggc
     4621 tggcggctgg tgcagcgcgg ggacccggcg gctttccgcg cgctggtggc ccagtgcctg
     4681 gtgtgcgtgc cctgggacgc acggccgccc cccgccgccc cctccttccg ccaggtgtcc
     4741 tgcctgaagg agctggtggc ccgagtgctg cagaggctgt gcgagcgcgg cgcgaagaac
     4801 gtgctggcct tcggcttcgc gctgctggac ggggcccgcg ggggcccccc cgaggccttc
     4861 accaccagcg tgcgcagcta cctgcccaac acggtgaccg acgcactgcg ggggagcggg
     4921 gcgtgggggc tgctgctgcg ccgcgtgggc gacgacgtgc tggttcacct gctggcacgc
     4981 tgcgcgctct ttgtgctggt ggctcccagc tgcgcctacc aggtgtgcgg gccgccgctg
     5041 taccagctcg gcgctgccac tcaggcccgg cccccgccac acgctagtgg accccgaagg
     5101 cgtctgggat gcgaacgggc ctggaaccat agcgtcaggg aggccggggt ccccctgggc
     5161 ctgccagccc cgggtgcgag gaggcgcggg ggcagtgcca gccgaagtct gccgttgccc
     5221 aagaggccca ggcgtggcgc tgcccctgag ccggagcgga cgcccgttgg gcaggggtcc
     5281 tgggcccacc cgggcaggac gcgtggaccg agtgaccgtg gtttctgtgt ggtgtcacct
     5341 gccagacccg ccgaagaagc cacctctttg gagggtgcgc tctctggcac gcgccactcc
     5401 cacccatccg tgggccgcca gcaccacgcg ggccccccat ccacatcgcg gccaccacgt
     5461 ccctgggaca cgccttgtcc cccggtgtac gccgagacca agcacttcct ctactcctca
     5521 ggcgacaagg agcagctgcg gccctccttc ctactcagct ctctgaggcc cagcctgact
     5581 ggcgctcgga ggctcgtgga gaccatcttt ctgggttcca ggccctggat gccagggact
     5641 ccccgcaggt tgccccgcct gccccagcgc tactggcaaa tgcggcccct gtttctggag
     5701 ctgcttggga accacgcgca gtgcccctac ggggtgctcc tcaagacgca ctgcccgctg
     5761 cgagctgcgg tcaccccagc agccggtgtc tgtgcccggg agaagcccca gggctctgtg
     5821 gcggcccccg aggaggagga cacagacccc cgtcgcctgg tgcagctgct ccgccagcac
     5881 agcagcccct ggcaggtgta cggcttcgtg cgggcctgcc tgcgccggct ggtgccccca
     5941 ggcctctggg gctccaggca caacgaacgc cgcttcctca ggaacaccaa gaagttcatc
     6001 tccctgggga agcatgccaa gctctcgctg caggagctga cgtggaagat gagcgtgcgg
     6061 gactgcgctt ggctgcgcag gagcccaggg gttggctgtg ttccggccgc agagcaccgt
     6121 ctgcgtgagg agatcctggc caagttcctg cactggctga tgagtgtgta cgtcgtcgag
     6181 ctgctcaggt ctttctttta tgtcacggag accacgtttc aaaagaacag gctctttttc
     6241 taccggaaga gtgtctggag caagttgcaa agcattggaa tcagacagca cttgaagagg
     6301 gtgcagctgc gggagctgtc ggaagcagag gtcaggcagc atcgggaagc caggcccgcc
     6361 ctgctgacgt ccagactccg cttcatcccc aagcctgacg ggctgcggcc gattgtgaac
     6421 atggactacg tcgtgggagc cagaacgttc cgcagagaaa agagggccga gcgtctcacc
     6481 tcgagggtga aggcactgtt cagcgtgctc aactacgagc gggcgcggcg ccccggcctc
     6541 ctgggcgcct ctgtgctggg cctggacgat atccacaggg cctggcgcac cttcgtgctg
     6601 cgtgtgcggg cccaggaccc gccgcctgag ctgtactttg tcaaggtgga tgtgacgggc
     6661 gcgtacgaca ccatccccca ggacaggctc acggaggtca tcgccagcat catcaaaccc
     6721 cagaacacgt actgcgtgcg tcggtatgcc gtggtccaga aggccgccca tgggcacgtc
     6781 cgcaaggcct tcaagagcca cgtctctacc ttgacagacc tccagccgta catgcgacag
     6841 ttcgtggctc acctgcagga gaccagcccg ctgagggatg ccgtcgtcat cgagcagagc
     6901 tcctccctga atgaggccag cagtggcctc ttcgacgtct tcctacgctt catgtgccac
     6961 cacgccgtgc gcatcagggg caagtcctac gtccagtgcc aggggatccc gcagggctcc
     7021 atcctctcca cgctgctctg cagcctgtgc tacggcgaca tggagaacaa gctgtttgcg
     7081 gggattcggc gggacgggct gctcctgcgt ttggtggatg atttcttgtt ggtgacacct
     7141 cacctcaccc acgcgaaaac cttcctcagg accctggtcc gaggtgtccc tgagtatggc
     7201 tgcgtggtga acttgcggaa gacagtggtg aacttccctg tagaagacga ggccctgggt
     7261 ggcacggctt ttgttcagat gccggcccac ggcctattcc cctggtgcgg cctgctgctg
     7321 gatacccgga ccctggaggt gcagagcgac tactccagct atgcccggac ctccatcaga
     7381 gccagtctca ccttcaaccg cggcttcaag gctgggagga acatgcgtcg caaactcttt
     7441 ggggtcttgc ggctgaagtg tcacagcctg tttctggatt tgcaggtgaa cagcctccag
     7501 acggtgtgca ccaacatcta caagatcctc ctgctgcagg cgtacaggtt tcacgcatgt
     7561 gtgctgcagc tcccatttca tcagcaagtt tggaagaacc ccacattttt cctgcgcgtc
     7621 atctctgaca cggcctccct ctgctactcc atcctgaaag ccaagaacgc agggatgtcg
     7681 ctgggggcca agggcgccgc cggccctctg ccctccgagg ccgtgcagtg gctgtgccac
     7741 caagcattcc tgctcaagct gactcgacac cgtgtcacct acgtgccact cctggggtca
     7801 ctcaggacag cccagacgca gctgagtcgg aagctcccgg ggacgacgct gactgccctg
     7861 gaggccgcag ccaacccggc actgccctca gacttcaaga ccatcctgga ctgaaaccca
     7921 gctttcttgt acaaagtggt ttagtaatga accggtccac caccaccacc accactaagg
     7981 atccggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     8041 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     8101 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     8161 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     8221 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     8281 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     8341 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     8401 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     8461 ccctcgttga ccgaatcacc gacctctctc cccagcaatt caccatgacc gagtacaagc
     8521 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8581 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8641 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8701 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8761 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8821 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8881 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8941 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     9001 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9061 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cgaaggtaga ggttctctcc
     9121 tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc tagactggac aagagcaaag
     9181 tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga
     9241 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     9301 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
     9361 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     9421 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     9481 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     9541 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     9601 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     9661 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     9721 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     9781 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     9841 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt
     9901 aactaagtaa ggatcgatcc aagatatcgt attcttaact atgttgctcc ttttacgcta
     9961 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10021 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10081 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10141 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10201 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10261 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
    10321 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10381 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10441 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac
    10501 tagaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    10561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
    10621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10801 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    10861 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    10921 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10981 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    11041 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    11101 tttt
//
