LOCUS       sequence-423194-       11104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Mutant TERT R865C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Reilly CR, Myllymaki M, Redd R, Padmanaban S, Karunakaran D, Tesmer 
            V, Tsai FD, Gibson CJ, Rana HQ, Zhong L, Saber W, Spellman SR, Hu 
            ZH, Orr EH, Chen MM, De Vivo I, DeAngelo DJ, Cutler C, Antin JH, 
            Neuberg D, Garber JE, Nandakumar J, Agarwal S, Lindsley RC
  TITLE     The clinical and functional effects of TERT variants in 
            myelodysplastic syndrome.
  JOURNAL   Blood. 2021 Sep 9;138(10):898-911. doi: 10.1182/blood.2021011075.
  PUBMED    34019641
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1182/blood"; 
            journalName: "Blood"; date: "2021-09-9- 9"; volume: "138"; issue: 
            "10"; pages: "898-911"
COMMENT     Sequence Label: TERT - R865C
FEATURES             Location/Qualifiers
     source          1..11104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     219..243
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    267..291
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             297..3695
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLCLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     protein_bind    complement(3697..3721)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3739..3756
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3766..4276
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4195..4213
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4286..4882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4286..4305)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4782..4802
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4883..4936
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4937..5554,5555..5560,5561..5596,5597..5599,5600..5635,
                     5636..5638,5639..5674,5675..5683)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5723..5743)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6141..6152)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6357..6590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6662..6783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6699..6718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6753..6772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6823..6958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6958..7029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7030..7098,7099..7890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7248..7267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8061..8649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8550..8569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8803..8820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8937..8958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8973..8978,8979..8996,8997..9003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9011..9027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9016..9038
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9035..9051
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9035..9051
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9070..9090
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9072..9090
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9118..9344
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9345..9525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9572..9697
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10190..10423
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10608..10652
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10865..10982
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11036..11104,1..246)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    11044..11062
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11080..11098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
       61 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      121 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      181 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      241 atcgcctgga gaattggcta gcatcaacaa gtttgtacaa aaaagcaggc ttcaccatgc
      301 cgcgcgctcc ccgctgccga gccgtgcgct ccctgctgcg cagccactac cgcgaggtgc
      361 tgccgctggc cacgttcgtg cggcgcctgg ggccccaggg ctggcggctg gtgcagcgcg
      421 gggacccggc ggctttccgc gcgctggtgg cccagtgcct ggtgtgcgtg ccctgggacg
      481 cacggccgcc ccccgccgcc ccctccttcc gccaggtgtc ctgcctgaag gagctggtgg
      541 cccgagtgct gcagaggctg tgcgagcgcg gcgcgaagaa cgtgctggcc ttcggcttcg
      601 cgctgctgga cggggcccgc gggggccccc ccgaggcctt caccaccagc gtgcgcagct
      661 acctgcccaa cacggtgacc gacgcactgc gggggagcgg ggcgtggggg ctgctgctgc
      721 gccgcgtggg cgacgacgtg ctggttcacc tgctggcacg ctgcgcgctc tttgtgctgg
      781 tggctcccag ctgcgcctac caggtgtgcg ggccgccgct gtaccagctc ggcgctgcca
      841 ctcaggcccg gcccccgcca cacgctagtg gaccccgaag gcgtctggga tgcgaacggg
      901 cctggaacca tagcgtcagg gaggccgggg tccccctggg cctgccagcc ccgggtgcga
      961 ggaggcgcgg gggcagtgcc agccgaagtc tgccgttgcc caagaggccc aggcgtggcg
     1021 ctgcccctga gccggagcgg acgcccgttg ggcaggggtc ctgggcccac ccgggcagga
     1081 cgcgtggacc gagtgaccgt ggtttctgtg tggtgtcacc tgccagaccc gccgaagaag
     1141 ccacctcttt ggagggtgcg ctctctggca cgcgccactc ccacccatcc gtgggccgcc
     1201 agcaccacgc gggcccccca tccacatcgc ggccaccacg tccctgggac acgccttgtc
     1261 ccccggtgta cgccgagacc aagcacttcc tctactcctc aggcgacaag gagcagctgc
     1321 ggccctcctt cctactcagc tctctgaggc ccagcctgac tggcgctcgg aggctcgtgg
     1381 agaccatctt tctgggttcc aggccctgga tgccagggac tccccgcagg ttgccccgcc
     1441 tgccccagcg ctactggcaa atgcggcccc tgtttctgga gctgcttggg aaccacgcgc
     1501 agtgccccta cggggtgctc ctcaagacgc actgcccgct gcgagctgcg gtcaccccag
     1561 cagccggtgt ctgtgcccgg gagaagcccc agggctctgt ggcggccccc gaggaggagg
     1621 acacagaccc ccgtcgcctg gtgcagctgc tccgccagca cagcagcccc tggcaggtgt
     1681 acggcttcgt gcgggcctgc ctgcgccggc tggtgccccc aggcctctgg ggctccaggc
     1741 acaacgaacg ccgcttcctc aggaacacca agaagttcat ctccctgggg aagcatgcca
     1801 agctctcgct gcaggagctg acgtggaaga tgagcgtgcg gggctgcgct tggctgcgca
     1861 ggagcccagg ggttggctgt gttccggccg cagagcaccg tctgcgtgag gagatcctgg
     1921 ccaagttcct gcactggctg atgagtgtgt acgtcgtcga gctgctcagg tctttctttt
     1981 atgtcacgga gaccacgttt caaaagaaca ggctcttttt ctaccggaag agtgtctgga
     2041 gcaagttgca aagcattgga atcagacagc acttgaagag ggtgcagctg cgggagctgt
     2101 cggaagcaga ggtcaggcag catcgggaag ccaggcccgc cctgctgacg tccagactcc
     2161 gcttcatccc caagcctgac gggctgcggc cgattgtgaa catggactac gtcgtgggag
     2221 ccagaacgtt ccgcagagaa aagagggccg agcgtctcac ctcgagggtg aaggcactgt
     2281 tcagcgtgct caactacgag cgggcgcggc gccccggcct cctgggcgcc tctgtgctgg
     2341 gcctggacga tatccacagg gcctggcgca ccttcgtgct gcgtgtgcgg gcccaggacc
     2401 cgccgcctga gctgtacttt gtcaaggtgg atgtgacggg cgcgtacgac accatccccc
     2461 aggacaggct cacggaggtc atcgccagca tcatcaaacc ccagaacacg tactgcgtgc
     2521 gtcggtatgc cgtggtccag aaggccgccc atgggcacgt ccgcaaggcc ttcaagagcc
     2581 acgtctctac cttgacagac ctccagccgt acatgcgaca gttcgtggct cacctgcagg
     2641 agaccagccc gctgagggat gccgtcgtca tcgagcagag ctcctccctg aatgaggcca
     2701 gcagtggcct cttcgacgtc ttcctacgct tcatgtgcca ccacgccgtg cgcatcaggg
     2761 gcaagtccta cgtccagtgc caggggatcc cgcagggctc catcctctcc acgctgctct
     2821 gcagcctgtg ctacggcgac atggagaaca agctgtttgc ggggattcgg cgggacgggc
     2881 tgctcctgtg tttggtggat gatttcttgt tggtgacacc tcacctcacc cacgcgaaaa
     2941 ccttcctcag gaccctggtc cgaggtgtcc ctgagtatgg ctgcgtggtg aacttgcgga
     3001 agacagtggt gaacttccct gtagaagacg aggccctggg tggcacggct tttgttcaga
     3061 tgccggccca cggcctattc ccctggtgcg gcctgctgct ggatacccgg accctggagg
     3121 tgcagagcga ctactccagc tatgcccgga cctccatcag agccagtctc accttcaacc
     3181 gcggcttcaa ggctgggagg aacatgcgtc gcaaactctt tggggtcttg cggctgaagt
     3241 gtcacagcct gtttctggat ttgcaggtga acagcctcca gacggtgtgc accaacatct
     3301 acaagatcct cctgctgcag gcgtacaggt ttcacgcatg tgtgctgcag ctcccatttc
     3361 atcagcaagt ttggaagaac cccacatttt tcctgcgcgt catctctgac acggcctccc
     3421 tctgctactc catcctgaaa gccaagaacg cagggatgtc gctgggggcc aagggcgccg
     3481 ccggccctct gccctccgag gccgtgcagt ggctgtgcca ccaagcattc ctgctcaagc
     3541 tgactcgaca ccgtgtcacc tacgtgccac tcctggggtc actcaggaca gcccagacgc
     3601 agctgagtcg gaagctcccg gggacgacgc tgactgccct ggaggccgca gccaacccgg
     3661 cactgccctc agacttcaag accatcctgg actgaaaccc agctttcttg tacaaagtgg
     3721 tttagtaatg aaccggtcca ccaccaccac caccactaag gatccggggt tggggttgcg
     3781 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     3841 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     3901 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     3961 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     4021 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     4081 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     4141 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     4201 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     4261 cgacctctct ccccagcaat tcaccatgac cgagtacaag cccacggtgc gcctcgccac
     4321 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4381 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4441 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4501 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4561 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4621 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4681 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4741 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4801 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4861 catgacccgc aagcccggtg ccgaaggtag aggttctctc ctcacttgtg gtgatgttga
     4921 agaaaaccct ggtccaatgt ctagactgga caagagcaaa gtcataaacg gagctctgga
     4981 attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     5041 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     5101 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     5161 gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga
     5221 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     5281 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     5341 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     5401 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     5461 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     5521 tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt
     5581 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     5641 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatcgatc
     5701 caagatatcg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6061 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6241 tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc
     6301 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6361 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6601 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6661 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6721 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6781 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6841 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6901 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt
     6961 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7561 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8761 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8821 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8881 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8941 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9001 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9061 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     9121 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     9181 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     9241 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     9301 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     9361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9541 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9601 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9661 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9721 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9781 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9841 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9901 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9961 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10021 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10081 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10141 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10201 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10261 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    10321 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    10381 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    10441 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    10501 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    10561 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    10621 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10681 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10741 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10801 cccacctccc aaccccgagg ggacaattct cgacctcgag acaaatggca gtattcatcc
    10861 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    10921 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    10981 ttcgggttta ttacagggac agcagagatc cactttggcc gcgaatcgat atgtcgagtt
    11041 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
    11101 gatg
//
