LOCUS       sequence-423211-       11816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK11A (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11816)
  TITLE     Gateway destination clones for generating protein-proximity networks
            using proximity-dependent biotinylation proteomics
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK11A_IDG-K
FEATURES             Location/Qualifiers
     source          1..11816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2217..2443
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2444..2624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2671..2796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3289..3522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5403..5423)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5424..5433
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5436..6206
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6213..6254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6255..6279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8638..8662)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8692..9280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8745..8765)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9163..9174)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9294..9793
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9329..9352)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9726..9745
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9814..10413
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9814..9833)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10310..10330
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10551..10784
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10856..10977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10893..10912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10947..10966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11017..11152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11172..11191)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11173..11191)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11201..11218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11201..11217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11210..11232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11359..11814
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11446..11465)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11656..11677
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     2221 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     2281 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     2341 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     2401 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     2461 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2521 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2581 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2641 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     6241 gcctggacag caccacaact ttgtacaaaa aagcaggctc caccatgggc gatgagaagg
     6301 actcttggaa ggtgaagaca ctggacgaga tcctgcagga gaagaagcgg agaaaggagc
     6361 aggaggagaa ggccgagatc aagcggctga agaacagcga cgatagggac tccaagcgcg
     6421 attctctgga ggagggcgag ctgcgggatc actgcatgga gatcaccatc agaaatagcc
     6481 cttacaggcg cgaggactcc atggaggata ggggcgagga ggacgattcc ctggccatca
     6541 agccccctca gcagatgtct cgcaaggaga aggtgcacca ccggaaggac gagaagagaa
     6601 aggagaagtg gaagcacgcc cgggtgaagg agagagagca cgagcggaga aagcggcaca
     6661 gagaggagca ggacaaggca aggagggagt gggagaggca gaagcggaga gagatggcaa
     6721 gggagcacag caggagggag agggatcgcc tggagcagct ggagcggaag agagagaggg
     6781 agcgcaagat gcgggagcag cagaaggagc agagggagca gaaggagagg gagaggagag
     6841 cagaggagag gcgcaaggag agagaggcaa ggagagaggt gtccgcccac caccggacaa
     6901 tgagagagga ttattccgac aaggtgaagg cctctcactg gtccaggtct ccaccaaggc
     6961 ctccaaggga gagattcgag ctgggcgacg gaaggaagcc aggagaggcc agaccagcac
     7021 cagcacagaa gcctgcacag ctgaaggaag aaaaaatgga ggagagggat ctgctgtccg
     7081 atctgcagga catcagcgat tccgagcgca agaccagctc cgccgagtct agctccgccg
     7141 agtctggaag cggatccgag gaagaagagg aagaagaaga agaggaagaa gaggagggct
     7201 ctacaagcga ggagtctgag gaagaagagg aggaagaaga agaggaggag gaggagaccg
     7261 gctccaactc tgaggaggca agcgagcagt ccgccgagga ggtgagcgag gaggagatgt
     7321 ccgaagacga agagagagag aacgagaatc acctgctggt ggtgcccgag agcaggtttg
     7381 atagggactc tggagagagc gaggaggcag aggaggaagt gggagagggc acacctcagt
     7441 ctagcgccct gaccgagggc gactacgtgc cagatagccc tgccctgctg ccaatcgagc
     7501 tgaagcagga gctgccaaag tatctgcccg ccctgcaggg atgcaggtcc gtggaggagt
     7561 tccagtgtct gaatcgcatc gaggagggca catacggcgt ggtgtatcgg gccaaggaca
     7621 agaagaccga tgagatcgtg gccctgaaga gactgaagat ggaaaaggag aaggagggct
     7681 ttccaatcac atccctgcgg gagatcaaca ccatcctgaa ggcccagcac cccaatatcg
     7741 tgacagtgag agagatcgtg gtgggctcca acatggacaa gatctacatc gtgatgaatt
     7801 atgtggagca cgatctgaag tctctgatgg agacaatgaa gcagccattc ctgcccggcg
     7861 aggtgaagac cctgatgatc cagctgctgc ggggcgtgaa gcacctgcac gacaactgga
     7921 tcctgcacag agatctgaag acctctaatc tgctgctgag ccacgccggc atcctgaagg
     7981 tcggcgactt tggactggca agggagtacg gaagccctct gaaggcctat acaccagtgg
     8041 tggtgaccca gtggtaccgc gcccctgagc tgctgctggg agccaaggag tattctacag
     8101 ccgtggacat gtggagcgtg ggctgtatct tcggcgagct gctgacccag aagcctctgt
     8161 ttccaggcaa ctccgagatc gatcagatca ataaggtgtt caaggagctg ggcacaccca
     8221 gcgagaagat ctggcctggc tactccgagc tgccagtggt gaagaagatg accttttctg
     8281 agcacccata taacaatctg aggaagcggt tcggcgccct gctgagcgac cagggctttg
     8341 atctgatgaa caagttcctg acctactttc caggcaggcg catcagcgcc gaggacggcc
     8401 tgaagcacga gtatttcagg gagacacctc tgccaatcga tccctctatg tttcccacct
     8461 ggcctgcaaa gagcgagcag cagagggtga agagaggcac aagccctagg ccacctgagg
     8521 gaggactggg atactcccag ctgggcgacg atgacctgaa ggagaccggc ttccacctga
     8581 ccacaaccaa tcagggagca tccgccgcag gaccaggctt ctctctgaag ttttaggacc
     8641 cagctttctt gtacaaagtt gtgatatcca gcacagtggc ggccgctcga caatcaacct
     8701 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8761 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8821 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8881 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8941 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9001 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9061 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     9121 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9181 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9241 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg
     9301 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     9361 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     9421 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     9481 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     9541 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     9601 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     9661 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     9721 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     9781 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     9841 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     9901 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     9961 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
    10021 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
    10081 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
    10141 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
    10201 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
    10261 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
    10321 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
    10381 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
    10441 accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac
    10501 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
    10561 agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
    10621 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10681 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10741 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
    10801 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
    10861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10981 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    11041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    11101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
    11161 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
    11221 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    11281 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    11341 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
    11401 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
    11461 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
    11521 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
    11581 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
    11641 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    11701 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    11761 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttag
//
