LOCUS       sequence-423214-       11171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RIOK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11171)
  TITLE     Gateway destination clones for generating protein-proximity networks
            using proximity-dependent biotinylation proteomics
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-RIOK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(111..179,180..971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1142..1730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1631..1650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1884..1901
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2018..2039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2054..2059,2060..2077,2078..2084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2092..2108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2097..2119
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2116..2132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2116..2132
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2151..2171
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2153..2171
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2199..2425
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2426..2606
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2653..2778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3271..3504
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3689..3733
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4000..4116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4150..4170
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4170..5381
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5218..5237
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5385..5405)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5406..5415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5418..6188
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6195..6236
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6237..6261
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7613..7624
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7975..7999)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8029..8617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8082..8102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8500..8511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8631..9130
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8666..8689)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9063..9082
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9151..9750
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9151..9170)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9647..9667
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9888..10121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10193..10314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10230..10249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10284..10303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10354..10489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10509..10528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10510..10528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10538..10555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10538..10554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10547..10569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10696..11151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10783..10802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10993..11014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
       61 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      241 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      301 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      361 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      421 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      481 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      541 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      601 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      661 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      721 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      781 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      841 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      901 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      961 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1801 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1861 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1921 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1981 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2041 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2101 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2161 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     2221 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     2281 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     2341 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     2401 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     2461 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2521 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2581 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2641 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2701 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2761 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     2821 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     2881 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     2941 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     3001 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     3061 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     3121 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     3181 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     3241 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     3301 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     3361 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     3421 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     3481 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3541 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3601 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3661 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3721 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3781 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     3841 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     3901 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     3961 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     4021 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4081 agaattacaa aaacaaatta caaaattcaa aattttatcg tccagtgtgg tggaattctg
     4141 cagatatcac aactttgtat agaaaagttg gcctccgcgc cgggttttgg cgcctcccgc
     4201 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     4261 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     4321 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     4381 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     4441 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     4501 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     4561 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
     4621 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
     4681 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
     4741 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     4801 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     4861 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     4921 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
     4981 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     5041 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     5101 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     5161 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     5221 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     5281 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     5341 tgtccgctaa attctggccg tttttggctt ttttgttaga cggccaactt ttgtatacaa
     5401 agtgggccac catgggaatc ccgctgctga acgctaaaca gattctggga cagctggacg
     5461 gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg ctggatcgaa
     5521 tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca gggagaggaa
     5581 gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt atgttttggc
     5641 gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt gtcatggcag
     5701 aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat gacctgtatc
     5761 tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg
     5821 cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg
     5881 tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg
     5941 ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg
     6001 ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga
     6061 tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc
     6121 tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg
     6181 ccgaaaaggc tagcggcaag cccatcccca accccctgct gggcctggac agcaccacaa
     6241 ctttgtacaa aaaagcaggc tccaccatgg actaccggcg gcttctcatg agccgggtgg
     6301 tccccgggca attcgacgac gcggactcct ctgacagtga aaacagagac ttgaagacag
     6361 tcaaagagaa ggatgacatt ctgtttgaag accttcaaga caatgtgaat gagaatggtg
     6421 aaggtgaaat agaagatgag gaggaggagg gttatgacga tgatgatgat gactgggact
     6481 gggatgaagg agttggaaaa ctcgccaagg gttatgtctg gaatggagga agcaacccac
     6541 aggcaaatcg acagacctcc gacagcagtt cagccaaaat gtctactcca gcagacaagg
     6601 tcttacggaa atttgagaat aaaattaatt tagataagct aaatgttact gattccgtca
     6661 taaataaagt caccgaaaag tctagacaaa aggaagcaga tatgtatcgc atcaaagata
     6721 aggcagacag agcaactgta gaacaggtgt tggatcccag aacaagaatg attttattca
     6781 agatgttgac tagaggaatc ataacagaga taaatggctg cattagcaca ggaaaagaag
     6841 ctaatgtata ccatgctagc acagcaaatg gagagagcag agcaatcaaa atttataaaa
     6901 cttctatttt ggtgttcaaa gatcgggata aatatgtaag tggagaattc agatttcgtc
     6961 atggctattg taaaggaaac cctaggaaaa tggtgaaaac ttgggcagaa aaagaaatga
     7021 ggaacttaat caggctaaac acagcagaga taccatgtcc agaaccaata atgctaagaa
     7081 gtcatgttct tgtcatgagt ttcatcggta aagatgacat gcctgcacca ctcttgaaaa
     7141 atgtccagtt atcagaatcc aaggctcggg agttgtacct gcaggtcatt cagtacatga
     7201 gaagaatgta tcaggatgcc agacttgtcc atgcagatct cagtgaattt aacatgctgt
     7261 accacggtgg aggcgtgtat atcattgacg tgtctcagtc cgtggagcac gaccacccac
     7321 atgccttgga gttcttgaga aaggattgcg ccaacgtcaa tgatttcttt atgaggcaca
     7381 gtgttgctgt catgactgtg cgggagctct ttgaatttgt cacagatcca tccattacac
     7441 atgagaacat ggatgcttat ctctcaaagg ccatggaaat agcatctcaa aggaccaagg
     7501 aagaacggtc tagccaagat catgtggatg aagaggtgtt taagcgagca tatattccta
     7561 gaaccttgaa tgaagtgaaa aattatgaga gggatatgga cataattatg aaattgaagg
     7621 aagaggacat ggccatgaat gcccaacaag ataatattct ataccagact gttacaggat
     7681 tgaagaaaga tttgtcagga gttcagaagg tccctgcact cctagaaaat caagtggagg
     7741 aaaggacttg ttctgattca gaagatattg gaagctctga gtgctctgac acagactctg
     7801 aagagcaggg agaccatgcc cgccccaaga aacacaccac ggaccctgac attgataaaa
     7861 aagaaagaaa aaagatggtc aaggaagccc agagagagaa aagaaaaaac aaaattccta
     7921 aacatgtgaa aaaaagaaag gagaagacag ccaagacgaa aaaaggcaaa taggacccag
     7981 ctttcttgta caaagttgtg atatccagca cagtggcggc cgctcgacaa tcaacctctg
     8041 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8101 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8161 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8221 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8281 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8341 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8401 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     8461 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8521 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8581 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga
     8641 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     8701 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     8761 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     8821 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     8881 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     8941 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     9001 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     9061 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     9121 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     9181 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     9241 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     9301 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     9361 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     9421 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     9481 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     9541 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     9601 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     9661 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     9721 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     9781 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     9841 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     9901 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     9961 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10021 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10081 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
    10141 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
    10201 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10261 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10321 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    10381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10441 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
    10501 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    10561 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    10621 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    10681 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    10741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    10801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    10861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    10921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    10981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    11041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    11101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
    11161 ttttcgggga a
//
