LOCUS       sequence-423216-       10467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFR receptor for split TEV assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Popovic L, Rossner MJ, Wehr MC
  TITLE     Protocol for identifying properties of ERBB receptor antagonists 
            using the barcoded ERBBprofiler assay.
  JOURNAL   STAR Protoc. 2024 Apr 16;5(2):102987. doi: 
            10.1016/j.xpro.2024.102987.
  PUBMED    38635397
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-04-16"; volume: "5(2):102987. doi"; pages: " 
            10.1016/j.xpro.2024.102987"
COMMENT     Sequence Label: pcDNA3_EGFR-NTEV-tcs-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..10467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(947..1018,1019..2881,2882..2950,2951..4576)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(4575..4599)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4994..5014
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5021..5050
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5060..5500
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5543..5563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5714..5947
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             5951..5977
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5981..6007
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6020..6038)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6021..6038)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6058..6075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6064..6288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6334..6762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6421..6440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6631..6652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6771..6791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6776..7105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6956..7091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7018..7037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7172..7966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7226..7245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7836..7855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8140..8261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8177..8196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8231..8250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8310..8326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8310..8326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8323..8345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8334..8350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8358..8364,8365..8382,8383..8388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8403..8424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8541..8558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8712..9300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8792..8811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9471..10262,10263..10331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10332..10436)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagtt ggcaccatgc gaccctccgg
      961 gacggccggg gcagcgctcc tggcgctgct ggctgcgctc tgcccggcga gtcgggctct
     1021 ggaggaaaag aaagtttgcc aaggcacgag taacaagctc acgcagttgg gcacttttga
     1081 agatcatttt ctcagcctcc agaggatgtt caataactgt gaggtggtcc ttgggaattt
     1141 ggaaattacc tatgtgcaga ggaattatga tctttccttc ttaaagacca tccaggaggt
     1201 ggctggttat gtcctcattg ccctcaacac agtggagcga attcctttgg aaaacctgca
     1261 gatcatcaga ggaaatatgt actacgaaaa ttcctatgcc ttagcagtct tatctaacta
     1321 tgatgcaaat aaaaccggac tgaaggagct gcccatgaga aatttacagg aaatcctgca
     1381 tggcgccgtg cggttcagca acaaccctgc cctgtgcaac gtggagagca tccagtggcg
     1441 ggacatagtc agcagtgact ttctcagcaa catgtcgatg gacttccaga accacctggg
     1501 cagctgccaa aagtgtgatc caagctgtcc caatgggagc tgctggggtg caggagagga
     1561 gaactgccag aaactgacca aaatcatctg tgcccagcag tgctccgggc gctgccgtgg
     1621 caagtccccc agtgactgct gccacaacca gtgtgctgca ggctgcacag gcccccggga
     1681 gagcgactgc ctggtctgcc gcaaattccg agacgaagcc acgtgcaagg acacctgccc
     1741 cccactcatg ctctacaacc ccaccacgta ccagatggat gtgaaccccg agggcaaata
     1801 cagctttggt gccacctgcg tgaagaagtg tccccgtaat tatgtggtga cagatcacgg
     1861 ctcgtgcgtc cgagcctgtg gggccgacag ctatgagatg gaggaagacg gcgtccgcaa
     1921 gtgtaagaag tgcgaagggc cttgccgcaa agtgtgtaac ggaataggta ttggtgaatt
     1981 taaagactca ctctccataa atgctacgaa tattaaacac ttcaaaaact gcacctccat
     2041 cagtggcgat ctccacatcc tgccggtggc atttaggggt gactccttca cacatactcc
     2101 tcctctggat ccacaggaac tggatattct gaaaaccgta aaggaaatca cagggttttt
     2161 gctgattcag gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat
     2221 catacgcggc aggaccaagc aacatggtca gttttctctt gcagtcgtca gcctgaacat
     2281 aacatccttg ggattacgct ccctcaagga gataagtgat ggagatgtga taatttcagg
     2341 aaacaaaaat ttgtgctatg caaatacaat aaactggaaa aaactgtttg ggacctccgg
     2401 tcagaaaacc aaaattataa gcaacagagg tgaaaacagc tgcaaggcca caggccaggt
     2461 ctgccatgcc ttgtgctccc ccgagggctg ctggggcccg gagcccaggg actgcgtctc
     2521 ttgccggaat gtcagccgag gcagggaatg cgtggacaag tgcaaccttc tggagggtga
     2581 gccaagggag tttgtggaga actctgagtg catacagtgc cacccagagt gcctgcctca
     2641 ggccatgaac atcacctgca caggacgggg accagacaac tgtatccagt gtgcccacta
     2701 cattgacggc ccccactgcg tcaagacctg cccggcagga gtcatgggag aaaacaacac
     2761 cctggtctgg aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac
     2821 ctacggatgc actgggccag gtcttgaagg ctgtccaacg aatgggccta agatcccgtc
     2881 catcgccact gggatggtgg gggccctcct cttgctgctg gtggtggccc tggggatcgg
     2941 cctcttcatg cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga
     3001 gagggagctt gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag
     3061 gatcttgaag gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac
     3121 ggtgtataag ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa
     3181 ggaattaaga gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt
     3241 gatggccagc gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac
     3301 cgtgcaactc atcacgcagc tcatgccctt cggctgcctc ctggactatg tccgggaaca
     3361 caaagacaat attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat
     3421 gaactacttg gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt
     3481 gaaaacaccg cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga
     3541 agagaaagaa taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc
     3601 aattttacac agaatctata cccaccagag tgatgtctgg agctacgggg tgaccgtttg
     3661 ggagttgatg acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc
     3721 catcctggag aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat
     3781 gatcatggtc aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat
     3841 catcgaattc tccaaaatgg cccgagaccc ccagcgctac cttgtcattc agggggatga
     3901 aagaatgcat ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga
     3961 agacatggac gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag
     4021 cagcccctcc acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc
     4081 caccgtggct tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt
     4141 cttgcagcga tacagctcag accccacagg cgccttgact gaggacagca tagacgacac
     4201 cttcctccca gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt
     4261 gcagaatcct gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta
     4321 ccaggacccc cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac
     4381 ctgtgtcaac agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat
     4441 tagcctggac aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg
     4501 catctttaag ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag
     4561 tgaatttatt ggagcaccaa ctttcttgta caaagtggtt gatgtcggag gtggaggtag
     4621 cggaggtgga ggtagcggag agagcttgtt taagggacca cgtgattaca acccgatatc
     4681 gagcaccatt tgtcatttga cgaatgaatc tgatgggcac acaacatcgt tgtatggtat
     4741 tggatttggt cccttcatca ttacaaacaa gcacttgttt cgtcgcaata atggaacact
     4801 gttggtccaa tcactacatg gtgtattcaa ggtcaaggac accacgactt tgcaacaaca
     4861 cctcattgat gggagggaca tgatgattat tcgcatgcct aaggatttcc caccatttcc
     4921 tcaaaagctg aaatttagag agccacaaag ggaagagcgc atatgtcttg tgacaaccaa
     4981 cttccaagct cccgagaacc tgtacttcca gggttctaga gaacagaagc tgatctcaga
     5041 agaggatctg gcccgggcca tgaagctact gtcttctatc gaacaagcat gcgatatttg
     5101 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     5161 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
     5221 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     5281 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     5341 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     5401 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     5461 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccggaattcc cggggatcag
     5521 cttgataatt cgattcacca tgcccaagaa gaagaggaag gtggcgtaca gccgcgcgcg
     5581 tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc
     5641 ccccgaagag gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg
     5701 cagactgtcg acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     5761 cgaggacgtc gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     5821 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     5881 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     5941 cggtgggggc tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     6001 ctatgcatga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     6061 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6121 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6181 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6241 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6301 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6361 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6421 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6481 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6541 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6601 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6661 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6721 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     6781 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6841 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6901 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7021 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     7081 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     7141 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     7201 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     7261 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     7321 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     7381 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7441 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7501 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     7561 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     7621 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     7681 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     7741 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7801 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7861 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7921 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7981 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     8041 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8101 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8221 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8281 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8341 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8401 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8461 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8521 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8581 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8641 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8701 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8761 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8821 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8881 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8941 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9001 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9061 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9121 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9181 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9241 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10381 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10441 tttccccgaa aagtgccacc tgacgtc
//
