LOCUS       sequence-423217-       10863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ERBB3 receptor for split TEV assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Popovic L, Rossner MJ, Wehr MC
  TITLE     Protocol for identifying properties of ERBB receptor antagonists 
            using the barcoded ERBBprofiler assay.
  JOURNAL   STAR Protoc. 2024 Apr 16;5(2):102987. doi: 
            10.1016/j.xpro.2024.102987.
  PUBMED    38635397
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-04-16"; volume: "5(2):102987. doi"; pages: " 
            10.1016/j.xpro.2024.102987"
COMMENT     Sequence Label: pcDNA3_ERBB3-NTEV-tcs-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..10863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4971..4995)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5390..5410
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5417..5446
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5456..5896
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5939..5959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6110..6343
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             6347..6373
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6377..6403
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6416..6434)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6417..6434)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6454..6471)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6460..6684
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6730..7158
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6817..6836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7027..7048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7167..7187)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7172..7501
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7352..7487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7414..7433
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7568..8362
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7622..7641)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8232..8251
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8536..8657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8573..8592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8627..8646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8706..8722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8706..8722)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8719..8741)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8730..8746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8754..8760,8761..8778,8779..8784))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8799..8820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8937..8954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9108..9696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9188..9207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9867..10658,10659..10727))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10490..10509
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10728..10832)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagtt ggcaccatga gggcgaacga
      961 cgctctgcag gtgctgggct tgcttttcag cctggcccgg ggctccgagg tgggcaactc
     1021 tcaggcagtg tgtcctggga ctctgaatgg cctgagtgtg accggcgatg ctgagaacca
     1081 ataccagaca ctgtacaagc tctacgagag gtgtgaggtg gtgatgggga accttgagat
     1141 tgtgctcacg ggacacaatg ccgacctctc cttcctgcag tggattcgag aagtgacagg
     1201 ctatgtcctc gtggccatga atgaattctc tactctacca ttgcccaacc tccgcgtggt
     1261 gcgagggacc caggtctacg atgggaagtt tgccatcttc gtcatgttga actataacac
     1321 caactccagc cacgctctgc gccagctccg cttgactcag ctcaccgaga ttctgtcagg
     1381 gggtgtttat attgagaaga acgataagct ttgtcacatg gacacaattg actggaggga
     1441 catcgtgagg gaccgagatg ctgagatagt ggtgaaggac aatggcagaa gctgtccccc
     1501 ctgtcatgag gtttgcaagg ggcgatgctg gggtcctgga tcagaagact gccagacatt
     1561 gaccaagacc atctgtgctc ctcagtgtaa tggtcactgc tttgggccca accccaacca
     1621 gtgctgccat gatgagtgtg ccgggggctg ctcaggccct caggacacag actgctttgc
     1681 ctgccggcac ttcaatgaca gtggagcctg tgtacctcgc tgtccacagc ctcttgtcta
     1741 caacaagcta actttccagc tggaacccaa tccccacacc aagtatcagt atggaggagt
     1801 ttgtgtagcc agctgtcccc ataactttgt ggtggatcaa acatcctgtg tcagggcctg
     1861 tcctcctgac aagatggaag tagataaaaa tgggctcaag atgtgtgagc cttgtggggg
     1921 actatgtccc aaagcctgtg agggaacagg ctctgggagc cgcttccaga ctgtggactc
     1981 gagcaacatt gatggatttg tgaactgcac caagatcctg ggcaacctgg actttctgat
     2041 caccggcctc aatggagacc cctggcacaa gatccctgcc ctggacccag agaagctcaa
     2101 tgtcttccgg acagtacggg agatcacagg ttacctgaac atccagtcct ggccgcccca
     2161 catgcacaac ttcagtgttt tttccaattt gacaaccatt ggaggcagaa gcctctacaa
     2221 ccggggcttc tcattgttga tcatgaagaa cttgaatgtc acatctctgg gcttccgatc
     2281 cctgaaggaa attagtgctg ggcgtatcta tataagtgcc aataggcagc tctgctacca
     2341 ccactctttg aactggacca aggtgcttcg ggggcctacg gaagagcgac tagacatcaa
     2401 gcataatcgg ccgcgcagag actgcgtggc agagggcaaa gtgtgtgacc cactgtgctc
     2461 ctctggggga tgctggggcc caggccctgg tcagtgcttg tcctgtcgaa attatagccg
     2521 aggaggtgtc tgtgtgaccc actgcaactt tctgaatggg gagcctcgag aatttgccca
     2581 tgaggccgaa tgcttctcct gccacccgga atgccaaccc atggagggca ctgccacatg
     2641 caatggctcg ggctctgata cttgtgctca atgtgcccat tttcgagatg ggccccactg
     2701 tgtgagcagc tgcccccatg gagtcctagg tgccaagggc ccaatctaca agtacccaga
     2761 tgttcagaat gaatgtcggc cctgccatga gaactgcacc caggggtgta aaggaccaga
     2821 gcttcaagac tgtttaggac aaacactggt gctgatcggc aaaacccatc tgacaatggc
     2881 tttgacagtg atagcaggat tggtagtgat tttcatgatg ctgggcggca cttttctcta
     2941 ctggcgtggg cgccggattc agaataaaag ggctatgagg cgatacttgg aacggggtga
     3001 gagcatagag cctctggacc ccagtgagaa ggctaacaaa gtcttggcca gaatcttcaa
     3061 agagacagag ctaaggaagc ttaaagtgct tggctcgggt gtctttggaa ctgtgcacaa
     3121 aggagtgtgg atccctgagg gtgaatcaat caagattcca gtctgcatta aagtcattga
     3181 ggacaagagt ggacggcaga gttttcaagc tgtgacagat catatgctgg ccattggcag
     3241 cctggaccat gcccacattg taaggctgct gggactatgc ccagggtcat ctctgcagct
     3301 tgtcactcaa tatttgcctc tgggttctct gctggatcat gtgagacaac accggggggc
     3361 actggggcca cagctgctgc tcaactgggg agtacaaatt gccaagggaa tgtactacct
     3421 tgaggaacat ggtatggtgc atagaaacct ggctgcccga aacgtgctac tcaagtcacc
     3481 cagtcaggtt caggtggcag attttggtgt ggctgacctg ctgcctcctg atgataagca
     3541 gctgctatac agtgaggcca agactccaat taagtggatg gcccttgaga gtatccactt
     3601 tgggaaatac acacaccaga gtgatgtctg gagctatggt gtgacagttt gggagttgat
     3661 gaccttcggg gcagagccct atgcagggct acgattggct gaagtaccag acctgctaga
     3721 gaagggggag cggttggcac agccccagat ctgcacaatt gatgtctaca tggtgatggt
     3781 caagtgttgg atgattgatg agaacattcg cccaaccttt aaagaactag ccaatgagtt
     3841 caccaggatg gcccgagacc caccacggta tctggtcata aagagagaga gtgggcctgg
     3901 aatagcccct gggccagagc cccatggtct gacaaacaag aagctagagg aagtagagct
     3961 ggagccagaa ctagacctag acctagactt ggaagcagag gaggacaacc tggcaaccac
     4021 cacactgggc tccgccctca gcctaccagt tggaacactt aatcggccac gtgggagcca
     4081 gagcctttta agtccatcat ctggatacat gcccatgaac cagggtaatc ttggggagtc
     4141 ttgccaggag tctgcagttt ctgggagcag tgaacggtgc ccccgtccag tctctctaca
     4201 cccaatgcca cggggatgcc tggcatcaga gtcatcagag gggcatgtaa caggctctga
     4261 ggctgagctc caggagaaag tgtcaatgtg taggagccgg agcaggagcc ggagcccacg
     4321 gccacgcgga gatagcgcct accattccca gcgccacagt ctgctgactc ctgttacccc
     4381 actctcccca cccgggttag aggaagagga tgtcaacggt tatgtcatgc cagatacaca
     4441 cctcaaaggt actccctcct cccgggaagg caccctttct tcagtgggtc tcagttctgt
     4501 cctgggtact gaagaagaag atgaagatga ggagtatgaa tacatgaacc ggaggagaag
     4561 gcacagtcca cctcatcccc ctaggccaag ttcccttgag gagctgggtt atgagtacat
     4621 ggatgtgggg tcagacctca gtgcctctct gggcagcaca cagagttgcc cactccaccc
     4681 tgtacccatc atgcccactg caggcacaac tccagatgaa gactatgaat atatgaatcg
     4741 gcaacgagat ggaggtggtc ctgggggtga ttatgcagcc atgggggcct gcccagcatc
     4801 tgagcaaggg tatgaagaga tgagagcttt tcaggggcct ggacatcagg ccccccatgt
     4861 ccattatgcc cgcctaaaaa ctctacgtag cttagaggct acagactctg cctttgataa
     4921 ccctgattac tggcatagca ggcttttccc caaggctaat gcccagagaa cgccaacttt
     4981 cttgtacaaa gtggttgatg tcggaggtgg aggtagcgga ggtggaggta gcggagagag
     5041 cttgtttaag ggaccacgtg attacaaccc gatatcgagc accatttgtc atttgacgaa
     5101 tgaatctgat gggcacacaa catcgttgta tggtattgga tttggtccct tcatcattac
     5161 aaacaagcac ttgtttcgtc gcaataatgg aacactgttg gtccaatcac tacatggtgt
     5221 attcaaggtc aaggacacca cgactttgca acaacacctc attgatggga gggacatgat
     5281 gattattcgc atgcctaagg atttcccacc atttcctcaa aagctgaaat ttagagagcc
     5341 acaaagggaa gagcgcatat gtcttgtgac aaccaacttc caagctcccg agaacctgta
     5401 cttccagggt tctagagaac agaagctgat ctcagaagag gatctggccc gggccatgaa
     5461 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc
     5521 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc
     5581 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga
     5641 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
     5701 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt
     5761 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt
     5821 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag
     5881 acagttgact gtatcgccgg aattcccggg gatcagcttg ataattcgat tcaccatgcc
     5941 caagaagaag aggaaggtgg cgtacagccg cgcgcgtacg aaaaacaatt acgggtctac
     6001 catcgagggc ctgctcgatc tcccggacga cgacgccccc gaagaggcgg ggctggcggc
     6061 tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac
     6121 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtcgcga tggcgcatgc
     6181 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     6241 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     6301 gcagatgttt accgatgccc ttggaattga cgagtacggt gggggctacc catacgatgt
     6361 tcctgactat gcgggctatc cctatgacgt cccggactat gcatgagggc cctattctat
     6421 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     6481 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6541 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6601 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6661 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6721 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6781 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6841 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6901 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6961 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7021 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7081 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7141 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7201 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7321 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7381 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7441 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7501 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     7561 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7621 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7681 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7741 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7801 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7861 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7921 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7981 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     8041 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     8101 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     8161 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     8221 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     8281 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     8341 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     8401 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8461 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8521 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8581 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8641 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     8701 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8761 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8821 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8881 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8941 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9001 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9061 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9121 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9181 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9241 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9301 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9361 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9421 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9481 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9541 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9601 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9721 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9781 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9841 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9901 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9961 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10021 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10081 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10141 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10201 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10261 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10321 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10381 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10441 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10501 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10561 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10621 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10681 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10741 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10801 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10861 gtc
//
