LOCUS       sequence-423218-        9261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Popovic L, Rossner MJ, Wehr MC
  TITLE     Protocol for identifying properties of ERBB receptor antagonists 
            using the barcoded ERBBprofiler assay.
  JOURNAL   STAR Protoc. 2024 Apr 16;5(2):102987. doi: 
            10.1016/j.xpro.2024.102987.
  PUBMED    38635397
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-04-16"; volume: "5(2):102987. doi"; pages: " 
            10.1016/j.xpro.2024.102987"
COMMENT     Sequence Label: pcDNA3_ERBB2-V5
FEATURES             Location/Qualifiers
     source          1..9261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     864..883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    912..936
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4702..4726)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4752..4793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4833..4852)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4877..4925
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5127..5555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5214..5233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5424..5445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5564..5584)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5569..5898
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5749..5884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5811..5830
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5965..6759
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6019..6038)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6629..6648
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6935..7056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6972..6991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7026..7045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7105..7121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7105..7121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7118..7140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7129..7145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7153..7159,7160..7177,7178..7183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7198..7219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7336..7353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7507..8095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7587..7606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8266..9057,9058..9126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8889..8908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9127..9231)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      901 ttaagctatc aacaagtttg tacaaaaaag ttggcaccat ggagctggcg gccttgtgcc
      961 gctgggggct cctcctcgcc ctcttgcccc ccggagccgc gagcacccaa gtgtgcaccg
     1021 gcacagacat gaagctgcgg ctccctgcca gtcccgagac ccacctggac atgctccgcc
     1081 acctctacca gggctgccag gtggtgcagg gaaacctgga actcacctac ctgcccacca
     1141 atgccagcct gtccttcctg caggatatcc aggaggtgca gggctacgtg ctcatcgctc
     1201 acaaccaagt gaggcaggtc ccactgcaga ggctgcggat tgtgcgaggc acccagctct
     1261 ttgaggacaa ctatgccctg gccgtgctag acaatggaga cccgctgaac aataccaccc
     1321 ctgtcacagg ggcctcccca ggaggcctgc gggagctgca gcttcgaagc ctcacagaga
     1381 tcttgaaagg aggggtcttg atccagcgga acccccagct ctgctaccag gacacgattt
     1441 tgtggaagga catcttccac aagaacaacc agctggctct cacactgata gacaccaacc
     1501 gctctcgggc ctgccacccc tgttctccga tgtgtaaggg ctcccgctgc tggggagaga
     1561 gttctgagga ttgtcagagc ctgacgcgca ctgtctgtgc cggtggctgt gcccgctgca
     1621 aggggccact gcccactgac tgctgccatg agcagtgtgc tgccggctgc acgggcccca
     1681 agcactctga ctgcctggcc tgcctccact tcaaccacag tggcatctgt gagctgcact
     1741 gcccagccct ggtcacctac aacacagaca cgtttgagtc catgcccaat cccgagggcc
     1801 ggtatacatt cggcgccagc tgtgtgactg cctgtcccta caactacctt tctacggacg
     1861 tgggatcctg caccctcgtc tgccccctgc acaaccaaga ggtgacagca gaggatggaa
     1921 cacagcggtg tgagaagtgc agcaagccct gtgcccgagt gtgctatggt ctgggcatgg
     1981 agcacttgcg agaggtgagg gcagttacca gtgccaatat ccaggagttt gctggctgca
     2041 agaagatctt tgggagcctg gcatttctgc cggagagctt tgatggggac ccagcctcca
     2101 acactgcccc gctccagcca gagcagctcc aagtgtttga gactctggaa gagatcacag
     2161 gttacctata catctcagca tggccggaca gcctgcctga cctcagcgtc ttccagaacc
     2221 tgcaagtaat ccggggacga attctgcaca atggcgccta ctcgctgacc ctgcaagggc
     2281 tgggcatcag ctggctgggg ctgcgctcac tgagggaact gggcagtgga ctggccctca
     2341 tccaccataa cacccacctc tgcttcgtgc acacggtgcc ctgggaccag ctctttcgga
     2401 acccgcacca agctctgctc cacactgcca accggccaga ggacgagtgt gtgggcgagg
     2461 gcctggcctg ccaccagctg tgcgcccgag ggcactgctg gggtccaggg cccacccagt
     2521 gtgtcaactg cagccagttc cttcggggcc aggagtgcgt ggaggaatgc cgagtactgc
     2581 aggggctccc cagggagtat gtgaatgcca ggcactgttt gccgtgccac cctgagtgtc
     2641 agccccagaa tggctcagtg acctgttttg gaccggaggc tgaccagtgt gtggcctgtg
     2701 cccactataa ggaccctccc ttctgcgtgg cccgctgccc cagcggtgtg aaacctgacc
     2761 tctcctacat gcccatctgg aagtttccag atgaggaggg cgcatgccag ccttgcccca
     2821 tcaactgcac ccactcctgt gtggacctgg atgacaaggg ctgccccgcc gagcagagag
     2881 ccagccctct gacgtccatc atctctgcgg tggttggcat tctgctggtc gtggtcttgg
     2941 gggtggtctt tgggatcctc atcaagcgac ggcagcagaa gatccggaag tacacgatgc
     3001 ggagactgct gcaggaaacg gagctggtgg agccgctgac acctagcgga gcgatgccca
     3061 accaggcgca gatgcggatc ctgaaagaga cggagctgag gaaggtgaag gtgcttggat
     3121 ctggcgcttt tggcacagtc tacaagggca tctggatccc tgatggggag aatgtgaaaa
     3181 ttccagtggc catcaaagtg ttgagggaaa acacatcccc caaagccaac aaagaaatct
     3241 tagacgaagc atacgtgatg gctggtgtgg gctccccata tgtctcccgc cttctgggca
     3301 tctgcctgac atccacggtg cagctggtga cacagcttat gccctatggc tgcctcttag
     3361 accatgtccg ggaaaaccgc ggacgcctgg gctcccagga cctgctgaac tggtgtatgc
     3421 agattgccaa ggggatgagc tacctggagg atgtgcggct cgtacacagg gacttggccg
     3481 ctcggaacgt gctggtcaag agtcccaacc atgtcaaaat tacagacttc gggctggctc
     3541 ggctgctgga cattgacgag acagagtacc atgcagatgg gggcaaggtg cccatcaagt
     3601 ggatggcgct ggagtccatt ctccgccggc ggttcaccca ccagagtgat gtgtggagtt
     3661 atggtgtgac tgtgtgggag ctgatgactt ttggggccaa accttacgat gggatcccag
     3721 cccgggagat ccctgacctg ctggaaaagg gggagcggct gccccagccc cccatctgca
     3781 ccattgatgt ctacatgatc atggtcaaat gttggatgat tgactctgaa tgtcggccaa
     3841 gattccggga gttggtgtct gaattctccc gcatggccag ggacccccag cgctttgtgg
     3901 tcatccagaa tgaggacttg ggcccagcca gtcccttgga cagcaccttc taccgctcac
     3961 tgctggagga cgatgacatg ggggacctgg tggatgctga ggagtatctg gtaccccagc
     4021 agggcttctt ctgtccagac cctgccccgg gcgctggggg catggtccac cacaggcacc
     4081 gcagctcatc taccaggagt ggcggtgggg acctgacact agggctggag ccctctgaag
     4141 aggaggcccc caggtctcca ctggcaccct ccgaaggggc tggctccgat gtatttgatg
     4201 gtgacctggg aatgggggca gccaaggggc tgcaaagcct ccccacacat gaccccagcc
     4261 ctctacagcg gtacagtgag gaccccacag tacccctgcc ctctgagact gatggctacg
     4321 ttgcccccct gacctgcagc ccccagcctg aatatgtgaa ccagccagat gttcggcccc
     4381 agcccccttc gccccgagag ggccctctgc ctgctgcccg acctgctggt gccactctgg
     4441 aaaggcccaa gactctctcc ccagggaaga atggggtcgt caaagacgtt tttgcctttg
     4501 ggggtgccgt ggagaacccc gagtacttga caccccaggg aggagctgcc cctcagcccc
     4561 accctcctcc tgccttcagc ccagccttcg acaacctcta ttactgggac caggacccac
     4621 cagagcgggg ggctccaccc agcaccttca aagggacacc tacggcagag aacccagagt
     4681 acctgggtct ggacgtgcca gtgccaactt tcttgtacaa agtggttgat ctagagggcc
     4741 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     4801 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     4861 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     4921 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     4981 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     5041 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
     5101 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5521 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5581 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5641 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5701 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5761 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5821 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5881 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5941 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6001 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6061 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6121 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6181 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6241 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6301 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6361 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6421 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6481 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6541 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6601 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6661 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6721 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     6781 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6841 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6901 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7081 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7141 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7261 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7321 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7381 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7441 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7501 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7561 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7621 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7681 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7741 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7801 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7861 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7921 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7981 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8041 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8101 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8161 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8221 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8281 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8341 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8401 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8461 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8521 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8581 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8641 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8701 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8761 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8821 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8881 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8941 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9001 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9061 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9121 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9181 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9241 ccgaaaagtg ccacctgacg t
//
