LOCUS       sequence-423219-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ERBB4 receptor for split TEV assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Popovic L, Rossner MJ, Wehr MC
  TITLE     Protocol for identifying properties of ERBB receptor antagonists 
            using the barcoded ERBBprofiler assay.
  JOURNAL   STAR Protoc. 2024 Apr 16;5(2):102987. doi: 
            10.1016/j.xpro.2024.102987.
  PUBMED    38635397
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-04-16"; volume: "5(2):102987. doi"; pages: " 
            10.1016/j.xpro.2024.102987"
COMMENT     Sequence Label: pcDNA3_ERBB4-NTEV-tcs-GV-2xHA
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4875..4899)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5294..5314
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5321..5350
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5360..5800
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5843..5863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6014..6247
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             6251..6277
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6281..6307
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6320..6338)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6321..6338)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6358..6375)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6364..6588
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6634..7062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6721..6740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6931..6952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7071..7091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7076..7405
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7256..7391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7318..7337
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7472..8266
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7526..7545)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8136..8155
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8440..8561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8477..8496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8531..8550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8610..8626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8610..8626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8623..8645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8634..8650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8658..8664,8665..8682,8683..8688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8703..8724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8841..8858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9012..9600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9092..9111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9771..10562,10563..10631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10394..10413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10632..10736)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctctacca tgaagccggc
      961 gacaggactt tgggtctggg tgagccttct cgtggcggcg gggaccgtcc agcccagcga
     1021 ttctcagtca gtgtgtgcag gaacggagaa taaactgagc tctctctctg acctggaaca
     1081 gcagtaccga gccttgcgca agtactatga aaactgtgag gttgtcatgg gcaacctgga
     1141 gataaccagc attgagcaca accgggacct ctccttcctg cggtctgttc gagaagtcac
     1201 aggctacgtg ttagtggctc ttaatcagtt tcgttacctg cctctggaga atttacgcat
     1261 tattcgtggg acaaaacttt atgaggatcg atatgccttg gcaatatttt taaactacag
     1321 aaaagatgga aactttggac ttcaagaact tggattaaag aacttgacag aaatcctaaa
     1381 tggtggagtc tatgtagacc agaacaaatt cctttgttat gcagacacca ttcattggca
     1441 agatattgtt cggaacccat ggccttccaa cttgactctt gtgtcaacaa atggtagttc
     1501 aggatgtgga cgttgccata agtcctgtac tggccgttgc tggggaccca cagaaaatca
     1561 ttgccagact ttgacaagga cggtgtgtgc agaacaatgt gacggcagat gctacggacc
     1621 ttacgtcagt gactgctgcc atcgagaatg tgctggaggc tgctcaggac ctaaggacac
     1681 agactgcttt gcctgcatga atttcaatga cagtggagca tgtgttactc agtgtcccca
     1741 aacctttgtc tacaatccaa ccacctttca actggagcac aatttcaatg caaagtacac
     1801 atatggagca ttctgtgtca agaaatgtcc acataacttt gtggtagatt ccagttcttg
     1861 tgtgcgtgcc tgccctagtt ccaagatgga agtagaagaa aatgggatta aaatgtgtaa
     1921 accttgcact gacatttgcc caaaagcttg tgatggcatt ggcacaggat cattgatgtc
     1981 agctcagact gtggattcca gtaacattga caaattcata aactgtacca agatcaatgg
     2041 gaatttgatc tttctagtca ctggtattca tggggaccct tacaatgcaa ttgaagccat
     2101 agacccagag aaactgaacg tctttcggac agtcagagag ataacaggtt tcctgaacat
     2161 acagtcatgg ccaccaaaca tgactgactt cagtgttttt tctaacctgg tgaccattgg
     2221 tggaagagta ctctatagtg gcctgtcctt gcttatcctc aagcaacagg gcatcacctc
     2281 tctacagttc cagtccctga aggaaatcag cgcaggaaac atctatatta ctgacaacag
     2341 caacctgtgt tattatcata ccattaactg gacaacactc ttcagcacaa tcaaccagag
     2401 aatagtaatc cgggacaaca gaaaagctga aaattgtact gctgaaggaa tggtgtgcaa
     2461 ccatctgtgt tccagtgatg gctgttgggg acctgggcca gaccaatgtc tgtcgtgtcg
     2521 ccgcttcagt agaggaagga tctgcataga gtcttgtaac ctctatgatg gtgaatttcg
     2581 ggagtttgag aatggctcca tctgtgtgga gtgtgacccc cagtgtgaga agatggaaga
     2641 tggcctcctc acatgccatg gaccgggtcc tgacaactgt acaaagtgct ctcattttaa
     2701 agatggccca aactgtgtgg aaaaatgtcc agatggctta cagggggcaa acagtttcat
     2761 tttcaagtat gctgatccag atcgggagtg ccacccatgc catccaaact gcacccaagg
     2821 gtgtaacggt cccactagtc atgactgcat ttactaccca tggacgggcc attccacttt
     2881 accacaacat gctagaactc ccctgattgc agctggagta attggtgggc tcttcattct
     2941 ggtcattgtg ggtctgacat ttgctgttta tgttagaagg aagagcatca aaaagaaaag
     3001 agccttgaga agattcttgg aaacagagtt ggtggaacca ttaactccca gtggcacagc
     3061 acccaatcaa gctcaacttc gtattttgaa agaaactgag ctgaagaggg taaaagtcct
     3121 tggctcaggt gcttttggaa cggtttataa aggtatttgg gtacctgaag gagaaactgt
     3181 gaagattcct gtggctatta agattcttaa tgagacaact ggtcccaagg caaatgtgga
     3241 gttcatggat gaagctctga tcatggcaag tatggatcat ccacacctag tccggttgct
     3301 gggtgtgtgt ctgagcccaa ccatccagct ggttactcaa cttatgcccc atggctgcct
     3361 gttggagtat gtccacgagc acaaggataa cattggatca caactgctgc ttaactggtg
     3421 tgtccagata gctaagggaa tgatgtacct ggaagaaaga cgactcgttc atcgggattt
     3481 ggcagcccgt aatgtcttag tgaaatctcc aaaccatgtg aaaatcacag attttgggct
     3541 agccagactc ttggaaggag atgaaaaaga gtacaatgct gatggaggaa agatgccaat
     3601 taaatggatg gctctggagt gtatacatta caggaaattc acccatcaga gtgacgtttg
     3661 gagctatgga gttactatat gggaactgat gacctttgga ggaaaaccct atgatggaat
     3721 tccaacgcga gaaatccctg atttattaga gaaaggagaa cgtttgcctc agcctcccat
     3781 ctgcactatt gacgtttaca tggtcatggt caaatgttgg atgattgatg ctgacagtag
     3841 acctaaattt aaggaactgg ctgctgagtt ttcaaggatg gctcgagacc ctcaaagata
     3901 cctagttatt cagggtgatg atcgtatgaa gcttcccagt ccaaatgaca gcaagttctt
     3961 tcagaatctc ttggatgaag aggatttgga agatatgatg gatgctgagg agtacttggt
     4021 ccctcaggct ttcaacatcc cacctcccat ctatacttcc agagcaagaa ttgactcgaa
     4081 taggagtgaa attggacaca gccctcctcc tgcctacacc cccatgtcag gaaaccagtt
     4141 tgtataccga gatggaggtt ttgctgctga acaaggagtg tctgtgccct acagagcccc
     4201 aactagcaca attccagaag ctcctgtggc acagggtgct actgctgaga tttttgatga
     4261 ctcctgctgt aatggcaccc tacgcaagcc agtggcaccc catgtccaag aggacagtag
     4321 cacccagagg tacagtgctg accccaccgt gtttgcccca gaacggagcc cacgaggaga
     4381 gctggatgag gaaggttaca tgactcctat gcgagacaaa cccaaacaag aatacctgaa
     4441 tccagtggag gagaaccctt ttgtttctcg gagaaaaaat ggagaccttc aagcattgga
     4501 taatcccgaa tatcacaatg catccaatgg tccacccaag gccgaggatg agtatgtgaa
     4561 tgagccactg tacctcaaca cctttgccaa caccttggga aaagctgagt acctgaagaa
     4621 caacatactg tcaatgccag agaaggccaa gaaagcgttt gacaaccctg actactggaa
     4681 ccacagcctg ccacctcgga gcacccttca gcacccagac tacctgcagg agtacagcac
     4741 aaaatatttt tataaacaga atgggcggat ccggcctatt gtggcagaga atcctgaata
     4801 cctctctgag ttctccctga agccaggcac tgtgctgccg cctccacctt acagacaccg
     4861 gaatactgtg gtggacccag ctttcttgta caaagtggtt gatgtcggag gtggaggtag
     4921 cggaggtgga ggtagcggag agagcttgtt taagggacca cgtgattaca acccgatatc
     4981 gagcaccatt tgtcatttga cgaatgaatc tgatgggcac acaacatcgt tgtatggtat
     5041 tggatttggt cccttcatca ttacaaacaa gcacttgttt cgtcgcaata atggaacact
     5101 gttggtccaa tcactacatg gtgtattcaa ggtcaaggac accacgactt tgcaacaaca
     5161 cctcattgat gggagggaca tgatgattat tcgcatgcct aaggatttcc caccatttcc
     5221 tcaaaagctg aaatttagag agccacaaag ggaagagcgc atatgtcttg tgacaaccaa
     5281 cttccaagct cccgagaacc tgtacttcca gggttctaga gaacagaagc tgatctcaga
     5341 agaggatctg gcccgggcca tgaagctact gtcttctatc gaacaagcat gcgatatttg
     5401 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     5461 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
     5521 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     5581 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     5641 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     5701 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     5761 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccggaattcc cggggatcag
     5821 cttgataatt cgattcacca tgcccaagaa gaagaggaag gtggcgtaca gccgcgcgcg
     5881 tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc
     5941 ccccgaagag gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg
     6001 cagactgtcg acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     6061 cgaggacgtc gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     6121 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     6181 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     6241 cggtgggggc tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     6301 ctatgcatga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     6361 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6421 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6481 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6541 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6601 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6961 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7021 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     7081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7321 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     7381 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     7441 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     7501 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     7561 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     7621 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     7681 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7741 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7801 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     7861 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     7921 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     7981 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     8041 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     8101 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     8161 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     8221 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     8281 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     8341 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8401 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8461 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8521 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8581 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8641 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8701 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8761 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8821 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8881 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8941 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9001 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9061 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9421 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9901 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10741 tttccccgaa aagtgccacc tgacgtc
//
