LOCUS       sequence-423232-       10574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual-enSERT-1 mCherry vector for site-specific integration into the 
            H11 locus (contains hsp68 minimal promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Hollingsworth EW, Liu TA, Alcantara JA, Chen CX, Jacinto SH, Kvon EZ
  TITLE     Rapid and quantitative functional interrogation of human enhancer 
            variant activity in live mice.
  JOURNAL   Nat Commun. 2025 Jan 6;16(1):409. doi: 10.1038/s41467-024-55500-7.
  PUBMED    39762235
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-6"; volume: "16(1):409. doi"; pages: " 
            10.1038/s41467-024-55500-7"
COMMENT     Sequence Label: PCR4-Hsp68::mCherry-H11
FEATURES             Location/Qualifiers
     source          1..10574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1302..1318
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1302..1318
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    1315..1449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1339..1358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1393..1412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1598..2308)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1876..1895)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2145..2163
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(5605..5624)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5606..5624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5631..5648)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5631..5647)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5640..5662)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5785..6087
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5966..5985
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6436..7230
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6436..7230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6490..6509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7100..7119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(7480..8271,8272..8340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8103..8122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8464..9052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8953..8972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9206..9223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9340..9361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9376..9381,9382..9399,9400..9406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9414..9430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9419..9441
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9438..9454
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9438..9454
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9475..9493
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaaaaaaaaa aaaaagagta aaggcatagt ctaccacatc cactgaggtg tgttcttaca
       61 gccaaagact ttttctcctg aggtctaagg gaacctcgta gtatgagtct catgacatgg
      121 aatgaatttc ctgttttgtt ttcttggcct tagttaagct aagcagaccc actattctgc
      181 accactcatt agttaggatg ctaacactca tttaagaggt taatgattga aaaggcattt
      241 tcacagaaac catatggcgc tccagtggct ttcattaggt catttgagaa tccagatttt
      301 ctcatcttct ctattcacta caggttgttg gcagttttgg ccagttagct caccttgaaa
      361 atggaaacat gtctgacaag agccttgagc tgaatatcat ccagagtatt tgctagagac
      421 agggtcttaa gtctcattaa attgcttaga agtgtgttta gtcctataaa ctatgtctca
      481 tttgtgtgtc cttcacaaag agcgctagtg tcgatcatca attagcctag cctataaagg
      541 tgacacaacc tggtcagtcc ctctgtatgt ctactatttc cccttctgat ttctactatc
      601 ttctcaagaa ctggttcttc agcttccttt gggaaatgtc actttttaat gatgtgtgtt
      661 ttgcttatgt atatgtctac atactaccca tgtgcttggt cactgcagag gccagaagac
      721 ggtgtcaggt gcactggaac tagagttaat gacagatgtg agccataggg tgctttgttc
      781 acatctttcc agtcccaaat gcgtctaaac ttatgtgata catactagat taccacaaaa
      841 ttgctccaaa gacacctttc tccctctgag atgaatctct ggctggcctt gctctaggca
      901 atcctgtgtt caattcaagg actgaatttg attacatcgg taatccagtg ccccaggcat
      961 ttctgctttt tctgtaaggt tcttatcccc tggaagactg tttacgtatt catttttttt
     1021 tgagactaag tttcaggtag aaaaagcttg ccttgaactt cactatatag ggcttatctt
     1081 gaactcttgt gggtcttcca cctttcttca gttagcttct gtacactgcc agacatgaaa
     1141 atcagatcca tttatagata gatggtcatg agccaccatg tgggtgtcta aaattgatct
     1201 caggacctct gaaagaccag ctagttctct taactgctga gccatctctc tagcgtgtct
     1261 atacacattt aatatcccct tgttcccttt ctgcttgccg ctctagaact agtggatcca
     1321 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1381 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     1441 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     1501 gaggtttttt aaagcaagta aaacctctac agatgtgata tggctgatta tgatcattac
     1561 ttatctagag cttagatccc ccctgcccta gttattatta cttgtacagc tcgtccatgc
     1621 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
     1681 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
     1741 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
     1801 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
     1861 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
     1921 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
     1981 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
     2041 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
     2101 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
     2161 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
     2221 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
     2281 tgttatcctc ctcgcccttg ctcaccatcg ccgcgctctg cttctggaag gctgcgctcc
     2341 gcggcgtgga tgctccgggg aaagttgcgc tccgcggcag ggatgctcct gggaaggttg
     2401 cgctccgcgg cagggatgct ctggggaagg ctggtcctgg ccgaggatcg ggaacgcgcc
     2461 gctcgctctg cttctcttgt cttcgcttgt ctctggatgg aaccagattt ggttctgagt
     2521 agctgtcagc gtctggtgac ctgctcgccg ccctgcgcct ttaaggagtc ttcaccggcc
     2581 ccgcccactc tccgctgggc caatcagcga gccggaggag gccttggggc caggaatctt
     2641 ccagcagttt cgcgtctggt ggagcttccc cgcctccctt gagtaatcgg agttgtgggt
     2701 tccgcccttg tccagaactc tccagaggtt tctggggttc actggagagt acggattcct
     2761 gagggggagg gtgtggggaa gtgctggtgc tactagtgac actgttgcta tggcgacgca
     2821 ttactaaggc ctgtgtggaa tggacaagaa agatcacctc tagctcggtg ttgtgtacag
     2881 tttgttgtga tttgtggggt ttcgccaact cgcacagttc tgaatatggg ggttaaaggc
     2941 taaaacttaa gggctaaaac ttctccccgc caagtttagg agacccaggg agatgcctgg
     3001 gggcgtgtcc ggtgacgtga tcctctccaa tcgcgttaca atggcagtgc tgcctctgac
     3061 ctcatggact aatttaggaa ctagaggctc tgtcccagca caggctcaaa gttgccggga
     3121 ggggcggggt ggggggtggg ggggaccccg gctgctcagt ttggatgttc ctggcggccg
     3181 ctgttccatc agcttcagcc tgaagagtaa gtagttctct attggcagtt tgacacatcc
     3241 tgcccttacc ttactaccac tgttggctca gcagacaccc aggataagtg cactagtgtt
     3301 cctttcctga ccagtgggac tgctttttcc agattcctct tgaagctgag gaggcagtga
     3361 gagcatgatg gcatctaatg agcttggaag taccagactg ccctgatcca cagccaggtt
     3421 ttgctgaaaa gtgaccagtt tgtcctcctc cagtagagtg ggcagctgaa ggattataat
     3481 ctactgtcaa gacttggagg cccctgcagt caaagtccaa tagaatatta tgaaatggag
     3541 aatggcttat tttaatctct atagtggaat taaaatagca tttatggccc cagatccata
     3601 attaatccaa tgactggtca aattagcttg aacctgactg aaaaacctgc aatcagtata
     3661 gtatctttca gaatgcttta cattcaattt ataataccag acttcaagtt gtaagttaac
     3721 aattttgaga gaaactaatg cagcagaggc aagggaaaga cttaaattaa tgtgaccatt
     3781 aattagttgg agtcaggctg gattaacatt gtgccagtaa atttcagaaa aattactata
     3841 tgacttctct gtaaactttg attttgtaga gtataatatt gattccttga tacttgccac
     3901 aagctacttt cagggttagt ccagcttaaa ctaatgctat cagaacttta tgtgcagaag
     3961 aaattccaga agtcctaagg taaaattaaa acgtgaaagg aactcattta gactgtctta
     4021 gttcatggaa gaaataaaca cagtaccagc catatcgttc acacacgtgg catatgtaac
     4081 ttttaataaa ccaaaagaaa aagtcccaaa tattcaagtg aaaaaaatcc aaacagttga
     4141 cacaagccta actgatagtt actttatgta cagctgtatg tataagttca aaaaaagcta
     4201 ccctatttgt atgtacaaaa gtttatacac aggtctgtac ataagggtct atacatttta
     4261 ttttttcaga acccttaggt gtcacctcta gaagacacca acacttcatt cacatatttt
     4321 ataaaagaaa gacttccagg actgacaatc ttgtatccct tgtatttgaa ccatgtaggt
     4381 tcattcgatt taacagcctt ctgtcataat gtagatcatg gtaggtatgt ggatccccta
     4441 aacatcttca catagtgtgc tccaatgtaa aagcccatta caaagaggga atgagagtca
     4501 tgaccagggt gtcaccacag gtccaggtat gggtgagcca gcctgccctc ttcactgtct
     4561 atattccagt tcatctacac tgatgacttt ggtgggcatg gagggcagag gctgctgtag
     4621 cacatctgag ccaaaccatt tggcaaggcc cacaggggag ctgctccttt ggctggtacg
     4681 atgctccagc tgggagtgca tgtggggcat gcccgaccgg ctgggtacat tctgaggagt
     4741 ctgaacgctg atagctgctg cctgggaact agagcctgga ggatgcagaa ctatggagag
     4801 aagagtgtca gtgcagcaaa gtagctctac tctacacctc acccacagaa ccccacaagt
     4861 ctactaagac cacccttgga tactcactcc caagtcctgt tctcagtatt gacaatattt
     4921 tctgacctac tttgcttgaa tggtttgtta ctttggggga tgggaagtct accagaggat
     4981 ttaaccaaag gcttcctaaa gtgcaaaatg caacgtaaga aaatgcaacg taagaaagcc
     5041 caccaaggct gacatgttca catctcagca aaaattctca ggcttataag aagtgaaaaa
     5101 cagagctaga gcatggctgg ctccgactgt ccttgcagta gtaataatcc ctgagactgc
     5161 acaggtcagt tcacttcacc tgtttttcta gtgacaccta agaaacaaag gggcagtgat
     5221 aactattgct cctggaaatc tcagaacttt ccagtgtgat cagctaacct ggcctatttc
     5281 taaaccttac ctgagcgctg tagctgctgc tgcatcaccg ccaaatgcag aggggtccca
     5341 ggacgagggt ttaggagagg gtgactagca gcagagacca aagggtacaa gggttgaccc
     5401 aggagtgggc cagatagccc ctgtaaagga gccatgtcta tcccaggagg aagcacacct
     5461 gatggggaga aagaagaaac tcatgagttg tagatgttcc taaaaatgtc aaagtttact
     5521 acttttgttg atttggatac ttacaaggag gtggatgtgg tgaacacttg ccaccataca
     5581 tgtaccaggg ctaaattcaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     5641 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5701 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5761 cagttgcgca gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt
     5821 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg
     5881 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     5941 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     6001 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     6061 attaacctga tgttctgggg aatataaatg tcaggcatga gattatcaaa aaggatcttc
     6121 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     6181 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     6241 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     6301 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     6361 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga
     6421 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6481 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6541 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6601 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6661 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6721 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6781 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6841 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6901 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6961 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7021 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7081 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7141 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7201 ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg
     7261 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg
     7321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7801 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7861 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7921 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7981 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8041 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8101 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8161 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8221 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8281 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgaccaaaat
     8401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9301 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9361 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9421 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tcagaattaa
     9481 ccctcactaa agggactagt cctgcaggtt taaacgaatt cattgactgc aggctgagtg
     9541 agtgggggta cgtaaggacc caaagctatg tagcctagct tctcaaggtg tctaaagaaa
     9601 ggaagggtag attaccaatg agcagagttt gggcttctct atttttaaag ttttaaaatg
     9661 tttatatcaa ggcaaggttt ctgttgtcct tgctggtctg gaactagatt ggccttgacc
     9721 taagagatga tgcgacttct gcctcccaag tgctaattaa aggtgtgagt caccacgctt
     9781 gccttggtgt tttctattta taggttatat attcattttt cctattgcat gtcacttccc
     9841 aaattacatc tcatctagtc atctgtgcac ccaatcacaa ataggcataa cctggtagat
     9901 ttccactaaa agttacttag aggaagtttg tgaatttatt attacaggca agccatggaa
     9961 atgtgtgtag ctgttgcagt tggtttgagg aggtgtaagt gcatggtgta tttggtagag
    10021 agactaggct gcctagttca ttatgagaag atatgtacaa cacaaacgaa gtagggtctc
    10081 aggttcaact gttaagcttt cttgcctcag gctgctggag agttgcactc gtacttattc
    10141 actcaattca ttctttggcc aggtgggtgg tggtggtggt ggttcacacc tttagtcata
    10201 gtgttgagga ggcagaggca agaggttctg tgtttgaggc cagctgggtc tatagattga
    10261 attctaggaa agccagggct acatagagaa acctgttttc aaaagaaaaa agaaaaaaag
    10321 aaaagacgaa tggtgtatgt gtgtgtttgt cagtcatctg tctggaattg aatttgagat
    10381 ccccctgcct cccaagtgtc ctgattaaag gcatagtcta tatagctggg agtggtggcg
    10441 cacactttta attctagcac ttgagaggca gaggcaggcg gatctctgag ttcgaggcca
    10501 gcctggtcaa caaagtgagt tccaggacag ccaaggctac acagagaaac cctgtctcga
    10561 aaaaacccaa aaaa
//
