LOCUS       sequence-423246-        8361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of SARS-CoV-2 Spike protein of 
            OMICRON.2P.BA5 variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Zhang QE, Lindenberger J, Parsons R, Thakur B, Parks R, Park CS, 
            Huang X, Sammour S, Janowska K, Spence TN, Edwards RJ, Martin M, 
            Williams WB, Gobeil S, Montefiori DC, Korber B, Saunders KO, Haynes 
            BF, Haynes BF, Henderson R, Acharya P
  TITLE     SARS-CoV-2 Omicron XBB lineage spike structures, conformations, 
            antigenicity, and receptor recognition.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 12:2024.02.12.580004. doi: 
            10.1101/2024.02.12.580004.
  PUBMED    38405707
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 12:2024.02.12.580004. doi: 
            10.1101/2024.02.12.580004."
COMMENT     Sequence Label: p-alpha-H-S-GSAS-2P-Omicron-BA.5
FEATURES             Location/Qualifiers
     source          1..8361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5471..5494
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5498..5521
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             5528..5551
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5591..5614
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(5716..5735)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5781..5836
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5835..5854)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6197..6213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6197..6213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6210..6232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6221..6237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6245..6251,6252..6269,6270..6275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6290..6311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6440..6457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6611..7199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6691..6710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7370..8161,8162..8230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7993..8012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8231..8335)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagytattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tgcggccgtc
     1741 tcaggccgag ttcggtaccg ccaccatgtt cgtgttcctg gtgctcctgc ctctggtgag
     1801 cagccagtgc gtgaacctga tcacccgaac ccagtcctac accaacagct ttacacgggg
     1861 cgtgtactac cctgacaagg tgttcagatc tagcgtcctg cacagcactc aggacctctt
     1921 cctgccgttc ttcagcaacg tgacatggtt ccacgccatc agcggcacaa acggaaccaa
     1981 gcggtttgat aaccccgtcc tgccattcaa tgatggagtt tacttcgcca gtaccgagaa
     2041 gagtaacatc atccggggct ggatcttcgg caccaccctg gatagcaaaa cacagagcct
     2101 cctgatcgtg aacaatgcca cgaacgtcgt gatcaaggtg tgcgagttcc agttttgcaa
     2161 tgatcctttc ctggatgtgt actaccacaa gaacaacaag agctggatgg aaagcgagtt
     2221 cagagtctac agcagcgcca acaactgcac attcgagtac gtctctcagc cttttctgat
     2281 ggaccttgag gggaaacaag gcaacttcaa gaacctgaga gaattcgtgt tcaagaacat
     2341 cgacggctac ttcaaaatct actccaagca cacacccatc aacctgggcc gggacctccc
     2401 tcagggcttc agcgccctgg aacccctggt cgacctgccc ataggcatca acataacgcg
     2461 gttccaaacc ctgctggccc tgcatagatc ctacctgact cctggcgaca gcagcagcgg
     2521 atggaccgcc ggagctgcag cctactatgt gggctacctg caacctagaa ccttcctgct
     2581 gaagtacaac gagaacggca caatcacaga cgccgtcgac tgcgccctgg accctctctc
     2641 tgagacaaag tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa
     2701 cttccgggtg cagcctacag agagcatcgt gcgatttcca aacattacca acctctgccc
     2761 cttcgacgag gtgtttaacg ccacaagatt tgcctccgtt tacgcctgga atagaaagag
     2821 aatcagcaat tgtgtggccg actactccgt gctgtataac ttcgcccctt tcttcgcctt
     2881 caagtgctac ggcgtttccc caacaaagct gaatgacctg tgcttcacca acgtgtacgc
     2941 cgactccttc gtaattagag gcaatgaggt gtcccagatc gcaccaggcc agaccggtaa
     3001 catcgctgac tacaactata agctgcctga tgattttaca ggctgcgtga tcgcctggaa
     3061 ctccaacaag ctggatagca aggtgggcgg caactacaac tacagatacc ggctgtttcg
     3121 caagtctaac ctgaaacctt tcgagagaga catctccaca gagatctacc aggccggtaa
     3181 caagccttgt aacggggtgg ccggcgtgaa ctgttacttc cctctgcaaa gctacggctt
     3241 ccggcctacc tatggagtcg gccaccagcc ataccgggtg gtcgtgctgt ccttcgagtt
     3301 actccacgcc cccgccaccg tctgcggtcc taagaagtcc accaatctgg ttaagaacaa
     3361 atgcgtgaac ttcaacttca acggcctgac cgggaccggc gtgctgaccg aaagcaacaa
     3421 aaagttcctc cccttccagc agttcggccg tgatatcgct gacaccacag atgccgtcag
     3481 agatccacag accctggaaa tcctggatat tacaccctgc tccttcggag gagtttctgt
     3541 gatcaccccc gggaccaata ccagcaacca ggtggctgtg ctgtaccaag gtgttaactg
     3601 caccgaggtt cctgtggcca tccacgccga tcagctgaca cctacttgga gagtgtactc
     3661 cactggctcc aatgtgttcc agaccagggc cggatgtctg atcggcgccg agtacgtgaa
     3721 taacagttac gagtgcgaca tccctatcgg cgccggcatc tgtgccagct accagaccca
     3781 gacaaagagc catgggtctg cttcctctgt agctagccag agcatcatcg cctacaccat
     3841 gagcctgggc gcagagaaca gcgtggccta ttccaacaac tctatcgcca ttcccaccaa
     3901 ctttacaatt agcgtcacaa cagagatcct gcccgtgagc atgaccaaga ccagcgtgga
     3961 ctgtacaatg tacatctgtg gcgacagcac tgaatgcagc aacctgctgc tgcaatacgg
     4021 ctccttttgc acccaactga agcgggcgct gaccggaatc gccgtggaac aggacaaaaa
     4081 tacccaggag gtgttcgccc aagtgaagca gatctacaag accccaccta tcaagtactt
     4141 cggcggcttt aactttagcc agattctccc tgatccttct aagcctagca agcggagctt
     4201 tatcgaggat ctgctgttca acaaggtcac cctggccgat gccggcttta tcaaacagta
     4261 tggcgattgt ctgggcgaca tagccgccag agatctgatc tgcgcccaga aattcaacgg
     4321 cctgacagtt ctcccacctc tgctgaccga cgagatgatc gctcagtaca cctctgccct
     4381 gctggctggc accatcacat ctgggtggac atttggcgcc ggcgccgccc tgcagatccc
     4441 ctttgccatg cagatggcct atagattcaa cggaatcggc gtgacccaga acgtgctgta
     4501 tgaaaaccag aagctgatcg ctaaccagtt caattctgcc atcggcaaga tccaggactc
     4561 cctctcctct accgccagcg ccctgggcaa actgcaggac gtggtgaatc acaacgccca
     4621 agccctgaac accctggtga agcagctcag cagcaagttt ggcgccatca gctctgtgct
     4681 gaacgatatc ctgtctagac tggaccctcc agaagccgaa gtccagatcg atagactgat
     4741 cacaggcaga ctgcagtccc tgcaaaccta cgtgacccaa cagctgatca gggccgctga
     4801 aataagagcc agcgccaatc tcgccgctac caagatgtcc gagtgtgtgc tgggacagtc
     4861 taaacgcgtt gacttctgcg gcaaaggcta tcacctgatg agcttccccc agagcgcgcc
     4921 gcacggcgtg gtgttcctgc atgtgacata cgtgcctgcc caagagaaga atttcacaac
     4981 cgcccctgcc atctgccacg acggcaaggc ccacttccca agagagggcg ttttcgtttc
     5041 caatggcaca cactggttcg tgacacaaag aaacttctac gaaccccaga ttatcaccac
     5101 cgacaacacc ttcgtgagtg gcaattgtga cgtggtcatc ggaatcgtga acaacacagt
     5161 gtacgaccct ctgcaacctg agctggactc ttttaaggaa gagctggaca agtactttaa
     5221 aaaccacacc agccccgatg tggacctggg cgacatcagt ggcattaacg ccagcgtggt
     5281 gaacatccaa aaggaaatcg acagactgaa cgaggtggcc aagaacctga acgagtccct
     5341 gatcgacctg caggagctcg gcaaatacga gcagggatcc ggatacatcc ccgaggcccc
     5401 cagagatggc caggcctacg tgcggaagga cggcgagtgg gtactgctga gcacattcct
     5461 gggcagatcc ctggaggtgc tgttccaggg cccaggccat caccaccatc accaccatca
     5521 tagcgcctgg tcccaccccc agttcgagaa gggcggcggt agtggagggg gcggatctgg
     5581 cggctcagct tggagccacc cccagttcga aaagtgataa tgactcgagc gataattcac
     5641 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     5701 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     5761 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     5821 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     5881 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     5941 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     6001 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     6061 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6121 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     6181 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6241 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6301 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6361 agcggatcga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8341 ttccccgaaa agtgccacct g
//
