LOCUS       sequence-423257-       13973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing Pad with T7 RNAP circuit variant T15. No seed promoter. 
            pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This 
            material contains 2 plasmids. Look below for more information..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 12638 to 12672)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 13973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pLP-pTac-Tn5-chlorR
FEATURES             Location/Qualifiers
     source          1..13973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..37
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18..36
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      6546..6632
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6667..6684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6801..7346)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6881..6900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7511..8302)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFGPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        8772..8849
                     /gene="lacI"
                     /label=lacI promoter
     CDS             8850..9932
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8869..8888)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    9945..9966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9981..9986,9987..10004,10005..10011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10019..10035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10024..10046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        12638..12672
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 aagcttgatg ggggatctaa tacgactcac tatagggaga tttgatagat taaaaaggaa
       61 aggaggaaag aaataatggc tcgtgtacag tttaaacaac gtgaatctac tgacgcaatc
      121 tttgttcact gctcggctac caagccaagt cagaatgttg gtgtccgtga gattcgccag
      181 tggcacaaag agcagggttg gctcgatgtg ggataccact ttatcatcaa gcgagacggt
      241 actgtggagg caggacgaga tgagatggct gtaggctctc acgctaaggg ttacaaccac
      301 aactctatcg gcgtctgcct tgttggtggt atcgacgata aaggtaagtt cgacgctaac
      361 tttacgccag cccaaatgca atcccttcgc tcactgcttg tcacactgct ggctaagtac
      421 gaaggcgctg tgcttcgcgc ccatcatgag gtggcgccga aggcttgccc ttcgttcgac
      481 cttaagcgtt ggtggggggg tttgagggcc aatggaacga aaacgtacgt ttatggtata
      541 acttattgat tataatacag tatacaaagg tcccttttca gggacccttt cgtatgttgt
      601 agctgatttt attttcttct tttcttttgg gtgcttttaa tagccatata tgatgtttca
      661 tataaagtta aaagtttccc catctgtcta tctatagcgt catgtttttc aggatttctt
      721 aatttctgca gcagtttaga tatatatttc gcaggtaact ttattattaa tgaattatgg
      781 gattgataca tttcctttgt ccaatctaaa tcttttcctg atttcaatac ctcactctca
      841 tctctattaa ttataaaacc gagctttcca tacggacctg ttaaatatga ttgtaattgt
      901 ctatactcag atggacctaa ttcttcaaaa tttttagcat caaaaacaac ttgtcttgtt
      961 ttatagtcct ccaatactcg tttccaaaaa tcagatttgc caccattggt gcctataata
     1021 tctcgtctct gaactgcgtt accatttgga tgggacttga tgtctgttag gtgggatgca
     1081 aatactattc ttagtgcgtc taaacaccat tgttcaaatt cagtggcacc ttcatttcct
     1141 atcggtatct gatctagatg agtagttatt tgacctattg ttttatttct aatggctgaa
     1201 ttatctgaaa taatattaat atcatattcg tcatttatct cctctgcttc ctctggagca
     1261 agtgcattac gatttagatt caaaccaagc cagtaacatg gatgaattaa taatttctca
     1321 ttactttcaa aacctttatc tggagttcgc ccgtcatggc aaaatgaata ggatgaggtg
     1381 tttttatcac gaataccaac aaatcctaca ctatacaaac tttggagaat tccacttgcc
     1441 tttaacaact gtatttcaga cgttatttta ggatctccat tttcttcgat taattcgaaa
     1501 gatgcttcta ttttttttaa gcacgtataa actgttaatt caggttcaat gctacgaaat
     1561 gcactagtta taacctgtat tgaaggaaag atcttctgat actctttcca gagatcttca
     1621 agtctggcca tggaaattga cttggctgca tattctaggt cagtgtttat gatagtttct
     1681 ctattctctc tgaatgcgga aaaaaaagct tcattcaaca atgatagtaa atccctgggc
     1741 cggtaaaggg taaattgcaa acatcgctta aaaccattcc tccctttaag atcatccgct
     1801 gtgcatctat cccaaactcg ttgatctttc tcaatatcta gcttaaatgc tactttcatt
     1861 cttttagctg acagcattag gagttgtgcc cagtcccaat gcaaccttat gacttgaccc
     1921 tctatatttc tcgagtaatc aggatcttcc tttgatagcg acctaaatat attatccctt
     1981 aaaaaaatta ttggacgaat gcattttgct ttttgattta attcaataga tgcatatgct
     2041 agacctgcaa tgattccaat tcctatatta tccggttcat acgcctcatc tagcttatcc
     2101 attaatatga caactttcct gtctgagcgt tcaagaagtg atactatatt attttctatt
     2161 tctgagatat tcaaattgaa ttgaagatca ccaattgatt cttctgggtt attttcatct
     2221 aaatactcct ttgcgacaag cctacacttt cttaaaatgt caccttgtgc agaattccat
     2281 tttttcaaat gttcattcaa caatgtttct gatgatattt gagatgacaa tttgtaatga
     2341 gatgatatat atgatgctat ctccattagc atagcgtatc gccataatag tcttgttgct
     2401 gcccttgcta aattaaatga tcctgtaaat ggtttcaaca ttgatctgaa accaataatt
     2461 tgagaatcgt ctggtgagaa actcaggatt aatatttttt tgtctttctt ccaatgctca
     2521 tttagctgaa taaataaagc acttttacct gtccctcgtc taccaacaac aatggtcctg
     2581 tcatcagttt caattagagt cctaaagtca gcagtttcaa tgaaagcatt actcaacatc
     2641 tttttatcat tttctgctgt cgtatcacca aacggattag actttgaagt aatattcaat
     2701 tccatattca accttttatg ttagttgctt tcatttatta ctttatatac tgttgaacga
     2761 gcaatattca ttgtttttga tatatgtgag gcacctaacc cctgttgcca catatttaat
     2821 actgcatctc tatctatttt tctttttcta ccaaaaacaa ctccttttgc cattgcctct
     2881 tggcgacctt cattggtacg ctctagtatt ctctgtcgtt ctgcctgggc cactgcagat
     2941 agaatagtga caaccatttt acccatctcc ccatcggtac tgattccgtc atcaataaac
     3001 cgaatggata caccttgggc gtcaaactct tttattaact ggatcatgtc agcagtatcg
     3061 cgcccaaggc ggtcaagttt cttcaccaag atgacgtcac cttcctccac cttcatcctc
     3121 agcaagtcca gccctttccg atcgcttgaa ctgcccgatg ccttgtcagt aaagatgcga
     3181 tttgctttca cgcctgcgtc tttgagtgcc cgaacctgaa tatcgagaga ttgctggctg
     3241 gttgataccc gtgcgtaacc aaaaagtcgc ataaaaatgt atcctaaatc aaatatcgga
     3301 caagcagtgt ctgttataac aaaaaatcga tttaatagac acattaacag cactgttttt
     3361 atgtgtgcga taatttataa tatttcggac ggttgcaaaa ttgttaatga ataactgtca
     3421 ggtagggagc ctgatatgcc tgtcgacttt ctaacctctg accaaaaaca gaattacggt
     3481 tgttatgctg cagaacctaa tgacgtgcaa ctggcgcgct attttcatct tgatgaacgg
     3541 gatctggcct tcattaacca acgacggggc aaacataata ggctgggcat tgcgcttcag
     3601 ctcaccacag cccgttttct gggaacattt ctgacggatt taactcaggt tctgcctggt
     3661 gttcaacatt ttgtcgcggt acagcttaat atccaccgtc cagaagttct ctcccgctat
     3721 gctgaacggg acactaccct tagagaacat actgcattaa ttaaggaata ttacggctat
     3781 catgaatttg gtgattttcc atggtctttc cgcctgaagc gtctgctata tacccgggcg
     3841 tggctcagta atgagcgacc gggtctgatg tttgattttg ccactgcatg gttgcttcaa
     3901 aataaggtat tactgcccgg agcaaccaca ctagtacgtc tcatcagtga aattcgtgaa
     3961 agggcaaatc agcggctgtg gaaaaagctg gccgcactgc cgaacaaatg gcaggcagct
     4021 caagtgatgg agcttctggt cattccggaa ggtcagcgtg tatcagcact ggaacagttg
     4081 aaaaaaggcc ctgttacagt cagtggacct gcgtttaatg aagcgctgga acgatatatc
     4141 cgattacgaa gtcttgagtt ttcccgactg aacttttccg gtctgcctgc cattcaactg
     4201 cgtaatctgg ctcgttatgc tggcatggcg tcggtaaaat atatcgctcg aatgccacag
     4261 cagagaaagc ttgctgtact tactgcattc gttaaagcac aggaaataac ggcattagac
     4321 gatgccgttg atgtgcttga tatgctaatt ctggacatta tccgcgaagc aaagaaaacc
     4381 gggcaaaaaa aaagactcag gacactgaaa gatcttgatc aggccgcatt gttactggcg
     4441 cgggcatgtg cattgttgct ggatgataat acagatgtcc cagatctcag gcaggttatc
     4501 ttcaagtgcg tacccaaaaa cagactggca gaatctgtaa gcaaggttaa tgaacttgct
     4561 cgtccacaga acaataattt ccatgacgaa atggttgagc agtacggtcg ggttaaacgt
     4621 tttcttccgg cggtgttgcg ggacctgcat ttccgtgcgg caccggcagg tgaacatgta
     4681 ctggctgcga ttcattatct ggcagaactg aatggttcga aaaagcgcat ccttgatgat
     4741 gcgcctgaac atattatcac cggtccctgg aaacgcctcg tatacgatgc ggagggacgg
     4801 atacagcgtg caggttattc actatgtttg ctggaacgcc ttcaggatgc actgcgccgc
     4861 cgggacatct ggcttgaaaa cagtgatcgc tggggagatc ctcgcgagaa gttgttgcaa
     4921 ggtgaagagt ggcagactca gcgtattcct gtctgtcggg cactgggaca tcctgtcgat
     4981 ggacgtaaag gtgtgcaaca actggctatt cagctggatg agacctggaa agccgtggca
     5041 tcacgatttg aaaagaatgc ggaagttcat atctgtaatg aaggtaaata tccatccctg
     5101 actatcagtt gtctggagaa acaggaagag ccaccatcat tgcttcgtct aaataatcgg
     5161 atcaaacagc tactcccacc ggtagattta acggaactgt tacttgagat agatgcccag
     5221 acaggattta cacatgagtt tgcgcatgtc agtgaatctg gtgctcgagc gcaagatttg
     5281 cacatcagtt tatgtgcggt attgatggct gaagcctgta atatcggact ggaaccgctg
     5341 ataaagcaca atataccagc actgacccgc catcggctca gttgggtgaa acagaattac
     5401 cttcgtgcag aaacgctggt cagcgccaat gcccgcctgg ttgattttca gtccacactg
     5461 gagcttgctg gtcgttgggg aggtggagaa gtggcatcag ctgacggcat gcgctttgtc
     5521 acaccagtga agaccatcaa ctcaggatct aacagaaaat attttggttc tggacgaggc
     5581 atcacctggt ataacttcgt atctgatcag tactctgggt tccatggcat tgtggtaccc
     5641 ggtacattac gggattcgat ttttgtactg gaaggacttc ttgagcagca gacagggctg
     5701 aatccagttg aaatcatgac agacactgcg ggtagcagcg atattatttt cggtctgttc
     5761 tggctactgg ggtatcagtt ttccccccgg cttgccgatg caggtgaggc ggtgttctgg
     5821 cgggtgaata aatcggcaaa ctacggagta ctggataagt tggcccgagg atatgcagat
     5881 ctgtcaaaag cggagagtca gtgggatgag atgatgcgaa ccgctggttc gctgaagttg
     5941 ggtacaattc atgcgtcaga actcattcgc tctttactga aaagctcgcg cccatcagga
     6001 ctggcacagg caattatgga agtagggcgt gtaaacaaaa cgctatatct cctcaattat
     6061 attgatgatg aagattatcg tcggcggatc ctgacgcagc ttaatcgagg agaaggccgc
     6121 cacgctgtgg ctcgtgcgat ttgttacgga caacgcggag agatcagaaa gcgttatcgt
     6181 gaagggcagg aagatcaact gggggcgctg ggcctggtta ctaatgcagt ggttctgtgg
     6241 aacacgctct atatggagga agcgttgagc tggatgcgcc gtaatggcga agaaattata
     6301 gatgaagata tcgctcggct atctcccctg atgcacgggc atatcaatat gttgggccat
     6361 tatacattca cgttgccaga ggatatttta aaaggggaac tgagagctct aaatttaaat
     6421 ataaacaacg aattatctcc ttaacgtacg ttttcgttcc attggccctc aaaccccgtg
     6481 ggagaagaac gaactggtca cttctgaccg tggataagat cccatggtac gcgtgctaga
     6541 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     6601 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag gggtatattc
     6661 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggtgag cggaaatggc
     6721 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     6781 agggccgcgg caaagccgtt tttccatagg cttcgccccc ctgacaagca tcacgaaatc
     6841 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6901 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     6961 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agtcccgggt aggcagttcg
     7021 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     7081 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     7141 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     7201 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     7261 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     7321 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     7381 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     7441 agttgttact agttgcagaa ataaaaaggc ctgcgattac cggcagtcct gttattagct
     7501 cagtaaagct ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     7561 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     7621 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     7681 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     7741 gcactacatt tcgctcatcg cctgcccagt cgggcggcga gttccatagc gttaaggttt
     7801 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     7861 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     7921 cgatcgtggc tggcccgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     7981 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     8041 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     8101 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     8161 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     8221 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     8281 cggcgatcac cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg
     8341 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     8401 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
     8461 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     8521 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct
     8581 tcatccgttt ccacggtgtg tgtcacccgg caaccttggg tagcagcgaa gtcgaggcat
     8641 ttctgtcctg gctgagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     8701 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgcgaaggc gaagcggcat
     8761 gcatttacgt tgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc
     8821 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag
     8881 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
     8941 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
     9001 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
     9061 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
     9121 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
     9181 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
     9241 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
     9301 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
     9361 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
     9421 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
     9481 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
     9541 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
     9601 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
     9661 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
     9721 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     9781 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     9841 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9901 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9961 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    10021 gtgagcggat aacaatttca cacaggaaac agctatgttt aatggtggca ttcgtcgctt
    10081 cgaagcagat caacaacgcc agattgcagc aggtagcgag agcgacacag catggaaccg
    10141 ccgcctgttg tcagaactta ttgcacctat ggctgaaggc attcaggctt ataaagaaga
    10201 gtacgaaggt aagaaaggtc gtgcacctcg cgcattggct ttcttacaat gtgtagaaaa
    10261 tgaagttgca gcatacatca ctatgaaagt tgttatggat atgctgaata cggatgctac
    10321 ccttcaggct attgcaatga gtgtagcaga acgcattgaa gaccaagtgc gcttttctaa
    10381 gctagaaggt cacgccgcta aatactttga gaaggttaag aagtcactca aggctagccg
    10441 tactaagtca tatcgtcacg ctcataacgt agctgtagtt gctgaaaaat cagttgcaga
    10501 aaaggacgcg gactttgacc gttgggaggc gtggccaaaa gaaactcaat tgcagattgg
    10561 tactaccttg cttgaaatct tagaaggtag cgttttctat aatggtgaac ctgtatttat
    10621 gcgtgctatg cgcacttatg gcggaaagac tatttactac ttacaaactt ctgaaagtgt
    10681 aggccagtgg attagcgcat tcaaagagca cgtagcgcaa ttaagcccag cttatgcccc
    10741 ttgcgtaatc cctcctcgtc cttggagaac tccatttaat ggagggttcc atactgagaa
    10801 ggtagctagc cgtatccgtc ttgtaaaagg taaccgtgag catgtacgca agttgactca
    10861 aaagcaaatg ccaaaggttt ataaggctat caacgcatta caaaatacac aatggcaaat
    10921 caacaaggat gtattagcag ttattgaaga agcaatccgc ttagaccttg gttatggtgt
    10981 accttccttc aagccactga ttgacaagga gaacaagcca gctaacccgg tacctgttga
    11041 attccaacac ctgcgcggtc gtgaactgaa agagatgcta tcacctgagc agtggcaaca
    11101 attcattaac tggaaaggcg aatgcgcgcg cctatatacc gcagaaacta agcgcggttc
    11161 aaagtccgcc gccgttgttc gcatggtagg acaggcccgt aaatatagcg cctttgaatc
    11221 catttacttc gtgtacgcaa tggatagccg cagccgtgtc tatgtgcaat ctagcacgct
    11281 ctctccgcag tctaacgact taggtaaggc attactccgc tttaccgagg gacgccctgt
    11341 gaatggcgta gaagcgctta aatggttctg catcaatggt gctaaccttt ggggatggga
    11401 caagaaaact tttgatgtgc gcgtgtctaa cgtattagat gaggaattcc aagatatgtg
    11461 tcgagacatc gccgcagacc ctctcacatt cacccaatgg gctaaagctg atgcacctta
    11521 tgaattcctc gcttggtgct ttgagtatgc tcaatacctt gatttggtgg atgaaggaag
    11581 ggccgacgaa ttccgcactc acctaccagt acatcaggac gggtcttgtt caggcattca
    11641 gcactatagt gctatgcttc gcgacgaagt aggggccaaa gctgttaacc tgaaaccctc
    11701 cgatgcaccg caggatatct atggggcggt ggcgcaagtg gttatcaaga agaatgcgct
    11761 atatatggat gcggacgatg caaccacgtt tacttctggt agcgtcacgc tgtccggtac
    11821 agaactgcga gcaatggcta gcgcatggga tagtattggt attacccgta gcttaaccaa
    11881 aaagcccgtg atgaccttgc catatggttc tactcgctta acttgccgtg aatctgtgat
    11941 tgattacatc gtagacttag aggaaaaaga ggcgcagaag gcagtagcag aagggcggac
    12001 ggcaaacaag gtacatcctt ttgaagacga tcgtcaagat tacttgactc cgggcgcagc
    12061 ttacaactac atgacggcac taatctggcc ttctatttct gaagtagtta aggcaccgat
    12121 agtagctatg aagatgatac gccagcttgc acgctttgca gcgaaacgta atgaaggcct
    12181 gatgtacacc ctgcctactg gcttcatctt agaacagaag atcatggcaa ccgagatgct
    12241 acgcgtgcgt acctgtctga tgggtgatat caagatgtcc cttcaggttg aaacggatat
    12301 cgtagatgaa gccgctatga tgggagcagc agcacctaat ttcgtacacg gtcatgacgc
    12361 aagtcacctt atccttaccg tatgtgaatt ggtagacaag ggcgtaacta gtatcgctgt
    12421 aatccacgac tcttttggta ctcatgcaga caacaccctc actcttagag tggcacttaa
    12481 agggcagatg gttgcaatgt atattgatgg taatgcgctt cagaaactac tggaggagca
    12541 tgaagagcgc tggatggttg atacaggtat cgaagtacct gagcaagggg agttcgacct
    12601 taacgaaatc atggattctg aatacgtatt tgcctaattg acggctagct cagtcctagg
    12661 tacagtgcta gcagctaaag ctatataatt taattaggag aagtaaaatg caggaaggcg
    12721 cgtatcgttt tattcgtaat ccgaacgtga gcgcggaagc gattcgtaaa gcgggtgcca
    12781 tgcagaccgt gaaactggcc caggaatttc cggaactgct ggcaattgaa gataccacct
    12841 ctctgagcta tcgtcatcag gtggcggaag aactgggcaa actgggtagc attcaggata
    12901 aaagccgtgg ttggtgggtg catagcgtgc tgctgctgga agcgaccacc tttcgtaccg
    12961 tgggcctgct gcatcaagaa tggtggatgc gtccggatga tccggcggat gcggatgaaa
    13021 aagaaagcgg caaatggctg gccgctgctg caacttcgcg tctgagaatg ggcagcatga
    13081 tgagcaacgt gattgcggtg tgcgatcgtg aagcggatat tcatgcgtat ctgcaagata
    13141 aactggccca taacgaacgt tttgtggtgc gtagcaaaca tccgcgtaaa gatgtggaaa
    13201 gcggcctgta tctgtatgat cacctgaaaa accagccgga actgggcggc tatcagatta
    13261 gcattccgca gaaaggcgtg gtggataaac gtggcaaacg taaaaaccgt ccggcgcgta
    13321 aagcgagcct gagcctgcgt agcggccgta ttaccctgaa acagggcaac attaccctga
    13381 acgcggtgct ggccgaagaa attaatccgc cgaaaggcga aaccccgctg aaatggctgc
    13441 tgctgaccag cgagccggtg gaaagtctgg cccaagcgct gcgtgtgatt gatatttata
    13501 cccatcgttg gcgcattgaa gaatttcaca aagcgtggaa aacgggtgcg ggtgcggaac
    13561 gtcagcgtat ggaagaaccg gataacctgg aacgtatggt gagcattctg agctttgtgg
    13621 cggtgcgtct gctgcaactg cgtgaatctt ttactccgcc gcaagcactg cgtgcgcagg
    13681 gcctgctgaa agaagcggaa cacgttgaaa gccagagcgc ggaaaccgtg ctgaccccgg
    13741 atgaatgcca actgctgggc tatctggata aaggcaaacg caaacgcaaa gaaaaagcgg
    13801 gcagcctgca atgggcgtat atggcgattg cgcgtctggg cggctttatg gatagcaaac
    13861 gtaccggcat tgcgagctgg ggtgcgctgt gggaaggttg ggaagcgctg caaagcaaac
    13921 tggatggctt tctggccgcg aaagacctga tggcgcaggg cattaaaatc taa
//
