LOCUS       sequence-423258-        1958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 2 ptac promoter part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Alker AT, Farrell MV, Aspiras AE, Dunbar TL, Fedoriouk A, Jones JE, 
            Mikhail SR, Salcedo GY, Moore BS, Shikuma NJ
  TITLE     A modular plasmid toolkit applied in marine bacteria reveals 
            functional insights during bacteria-stimulated metamorphosis.
  JOURNAL   mBio. 2023 Aug 2:e0150223. doi: 10.1128/mbio.01502-23.
  PUBMED    37530556
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2023 
            Aug 2:e0150223. doi: 10.1128/mbio.01502-23."
COMMENT     Sequence Label: pMMK201
FEATURES             Location/Qualifiers
     source          1..1958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(764..769,770..785,786..792)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    800..816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     805..827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      complement(971..1005)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1076..1735)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1650..1669
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1917..1958,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcaaac
      661 gccagtgtgt tcgtgtcatc taggacgggc ggcactcccg ttctggataa tgttttttgc
      721 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttggcaa ttaatcatcg
      781 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagaattcga
      841 gctcggtacc cggggatcct ctagatttaa gaaggagata tacatatgag taaaggagaa
      901 gaacttttca ctggagttgt ccccatagga tatataattc aattaagaat acctatgtga
      961 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1021 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1081 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1141 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1201 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1261 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     1321 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1381 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1441 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1501 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1561 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1621 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1681 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1741 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1801 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     1861 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1921 gatccttttt ttctgcgcgt aatctgctgc ttgcaaac
//
