LOCUS       sequence-423265-        4440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Xanthomonas translucens pv. phlei. LRD-modifying 
            cytochrome P450 enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Lin GM, Voigt CA
  TITLE     Design of a redox-proficient Escherichia coli for screening 
            terpenoids and modifying cytochrome P450s
  JOURNAL   Nature Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5"
COMMENT     Sequence Label: pCYP38
FEATURES             Location/Qualifiers
     source          1..4440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(296..841)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(930..1745)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(930..1745)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1653..1672
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1737..1756
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1922..1949
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1980..2007
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     primer_bind     complement(4352..4371)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttatttg tattgattaa
     2101 ggaggatctt atgcgtgtgg agggcgacag atatgacgtt atcgacatga gtgtggccca
     2161 gagccagctg gataatgcgt cctcaagcat tctgcagtgg gatggcgtcg cacgcgtggc
     2221 cttaccgggc aatgtggtta cgtgggccgc agggcgacac cagaccttga aatgtattct
     2281 ggcggatcag cgtttctccc gcgattggcg acattggcgc gctctccagg atggggagat
     2341 tccggaggat catcctctga ttggaatgtg tcgcttggat aacatggtga ctgcgcacgg
     2401 tgctgatcat cagcggctgc gtggcctgct ggcacgttcc ttcgcacctt cgcggatcgc
     2461 gttattagca ccagacatcg agcgtcgtgt tgaccagttg ttggcgggca ttgctgcgcg
     2521 tgggagcgcg gatctgatgc aggagttcgc cgttccgctg ccagcgagcg ttattgctga
     2581 gttatttgga ttgccggagg aaaatcgcgc agaaatcatt ctgctgacca atagcctggc
     2641 ctcgacaagc gcttctgcgg cagaagtgca gcaaacgcgc caacgcattc ctgacttctt
     2701 tcgctggctg attgcgctga aacgtcgtcg ccccggtgat gatctggcca gcgctctgat
     2761 tgcggcgcgc gattctggag aattagtgag cgatacggaa cttatagaca tgttgtttat
     2821 ggtactgtcg gcgggctttg tgaccacgac gggtgttatc ggaaacggcg tcctggcgtt
     2881 gcttacgcat ccccaacagc tgcgtctggt gctgagcgga caggtgccgt gggcacaggc
     2941 tatcgaagaa atcttacgct ggggcagctc ggtcaccaac ctgccgtttc ggtacgcaac
     3001 ccaggatgtt gaaatcgatg gctgcaccat ccgccgtggc gaggctatcc tcatggcgtt
     3061 tcacgctgcc aatcgtgacg aacgagcgtt tggacccggt gcagaagcct ttgatgttac
     3121 gcgtcaacct aacccgcacc tgagctttgg tcagggccca catttctgtt tgggcgctgc
     3181 actggcgcgc ctggaactgt tgtatgcctt cccagcgtta tttgcacgct tacacgagct
     3241 cgcgctggcg gttgccttag aggacattgc ataccaaccc agctatgtaa tccgttgccc
     3301 gcaacgcctg ccggtgacct ttcgtccaag cattgcccgt aaataataag gatgagctct
     3361 acaaataagc agaggtggtt gtgttgcgaa aaaaaaaaaa aaacacccta acgggtgttt
     3421 tttttttttt ggtgtcccca cgtgtggcgc tggagaccgt ccaatggcgg cgcgccatcg
     3481 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
     3541 tggtgaatat gagcccgaaa cgtcgtaccc aggcagaacg tgcaatggaa acccagggta
     3601 aactgattgc agcagcactg ggtgttctgc gtgaaaaagg ttatgcaggt tttcgtattg
     3661 cagatgttcc gggtgcagcc ggtgttagcc gtggtgcaca gagccatcat tttccgacca
     3721 aactggaact gctgctggca acctttgaat ggctgtatga gcagattacc gaacgtagcc
     3781 gtgcacgtct ggcaaaactg aaaccggaag atgatgttat tcagcagatg ctggatgatg
     3841 cagcagattt ttttctggat gatgatttta gcatcggcct ggatctgatt gttgcagcag
     3901 atcgtgatcc ggcactgcgt gaaggtattc tgcgtaccgt tgaacgtaat cgttttgttg
     3961 ttgaagatat gtggctgggt gtgctggtga gccgtggtct gagccgtgat gatgccgaag
     4021 atattctgtg gctgattttt aacagcgttc gtggtctgac agttcgtagc ctgtggcaga
     4081 aagataaaga acgttttgaa cgtgtgcgta atagcaccct ggaaattgca cgtgaacgtt
     4141 atgcaaaatt caaacgttga taaggatcct aattggtaac gaatcagaca attgacggct
     4201 cgagggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag gcacattatg
     4261 catcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat cgtggccggc
     4321 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     4381 gatcgggctc gccacttcgg gctcatgagc aaatatttta tctgaggtgc ttcctcgctc
//
