LOCUS       sequence-423272-        4390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Saccharothrix texasensis LRD-modifying cytochrome P450
            enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Lin GM, Voigt CA
  TITLE     Design of a redox-proficient Escherichia coli for screening 
            terpenoids and modifying cytochrome P450s
  JOURNAL   Nature Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5"
COMMENT     Sequence Label: pCYP39
FEATURES             Location/Qualifiers
     source          1..4390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(296..841)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(930..1745)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(930..1745)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1653..1672
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1737..1756
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1922..1949
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1980..2007
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     primer_bind     complement(4302..4321)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaattc actcaataag
     2101 gagctaccca aatgattcgt tttcctctgc cgccggcacc atcgttgcac catccgagcc
     2161 cggaatacgc ccgtctgcgc gctgaggacc cggtgccaga agttgaattg cctgatggta
     2221 gcccagcgta tctggcgact cgtcatgctg atgtacgcac ggtgttttca gacccgcggt
     2281 ttagtcgtgc cgctgcgact ggccctaaag gtccggagtg ggagctgggt tcattagcag
     2341 atgattcgct gctgggaatg gacccgccgt tgcataccaa agtgcgtcgt gtagtaggct
     2401 ctgcttttac tgctcgccga gttgaggatc tgcgcgccac cgttgccggc ctggtggatg
     2461 ggatggtgga tcgcttggag gaagcgggtc ctggggctga cctgtctgcc cacgtttcgg
     2521 cgccactccc gattgccgtg atctctgggc tgttcggtgt cgccgatgaa gatcaaggtc
     2581 gtgtgaaaga atggtctgac gcgttagtag gtgactggca agcggacccg ggtccgccac
     2641 gtgctgcact ggcaagtttc ggggagctga tagaaaaacg ccgcgccaac cctggcgacg
     2701 atttgatgag cgcgctggtc gcggcttggg accgagaaga tagtctgagc gaacgcgaac
     2761 ttgtttcggt gacggccggc ctgttagctg gcgggcacga aagcaccacc aatcaaatca
     2821 atctgttcgt tacagttttg tccagccacc tcgatcaact ggctgccctt agaggcgacg
     2881 accctcatgc agtcgcagtc gccgtggaag aactgacccg ctacatacaa ctgggtgaga
     2941 gtgggatctt gatgcctcgt gtagctctcg aggatgtcga tttgggcggc gtgacggtgc
     3001 cagcggggtc ggttgtgctt ccagcagtgg tcagtgcgaa ccgcgacgaa tcagtttttc
     3061 cggatgcgga tcgcctcgac ctgacgcgta gcccgaatcc tcacattggc tatggcgccg
     3121 gcgcgcatca ttgcatcggt gcgtcactcg ccagaatgga actgcaggaa gccctgggtg
     3181 gactgctgcg ccgcttacca aacctgcgcc tggcagtgcc ggaatctgag ctgcgtctga
     3241 aaccaggtct ggtagtgcga tcctttcagg aattgccggt aacctgggat cgctaataag
     3301 gatgagctct acaaataagc agaggtggtt gtgttgcgaa aaaaaaaaaa aaacacccta
     3361 acgggtgttt tttttttttt ggtgtcccca cgtgtggcgc tggagaccgt ccaatggcgg
     3421 cgcgccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
     3481 caattcaggg tggtgaatat gagcccgaaa cgtcgtaccc aggcagaacg tgcaatggaa
     3541 acccagggta aactgattgc agcagcactg ggtgttctgc gtgaaaaagg ttatgcaggt
     3601 tttcgtattg cagatgttcc gggtgcagcc ggtgttagcc gtggtgcaca gagccatcat
     3661 tttccgacca aactggaact gctgctggca acctttgaat ggctgtatga gcagattacc
     3721 gaacgtagcc gtgcacgtct ggcaaaactg aaaccggaag atgatgttat tcagcagatg
     3781 ctggatgatg cagcagattt ttttctggat gatgatttta gcatcggcct ggatctgatt
     3841 gttgcagcag atcgtgatcc ggcactgcgt gaaggtattc tgcgtaccgt tgaacgtaat
     3901 cgttttgttg ttgaagatat gtggctgggt gtgctggtga gccgtggtct gagccgtgat
     3961 gatgccgaag atattctgtg gctgattttt aacagcgttc gtggtctgac agttcgtagc
     4021 ctgtggcaga aagataaaga acgttttgaa cgtgtgcgta atagcaccct ggaaattgca
     4081 cgtgaacgtt atgcaaaatt caaacgttga taaggatcct aattggtaac gaatcagaca
     4141 attgacggct cgagggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag
     4201 gcacattatg catcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat
     4261 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     4321 cgatggggaa gatcgggctc gccacttcgg gctcatgagc aaatatttta tctgaggtgc
     4381 ttcctcgctc
//
