LOCUS       sequence-423290-       10479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DCAF1. C-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_DCAF1:1-1507
FEATURES             Location/Qualifiers
     source          1..10479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2296..2307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5654..6166
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6242..6260)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6297..6418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6308..6327)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6362..6381
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6663..7020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6663..6854
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6686..6706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6871..7006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7051..8088
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8067..8075
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8112..8160
                     /note="synthetic polyadenylation signal"
     promoter        8316..8420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8421..8489,8490..9281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8639..8658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9439..10027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9519..9538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10143..10426
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcgc
     1081 caccatgact acagtagtgg tacatgtgga ctccaaagct gagctcacta ccctgctgga
     1141 gcagtgggaa aaggaacatg gcagtgggca ggacatggta cctatcctta ccaggatgtc
     1201 tcaattgatt gaaaaagaaa ctgaagagta tcgtaaaggg gatccagacc catttgatga
     1261 tcgacatcct ggtcgagctg atccagagtg tatgctgggc cacttgctga gaatactctt
     1321 caagaatgat gatttcatga atgcactggt gaatgcatat gtgatgacaa gccgagagcc
     1381 ccctttaaac actgcagctt gcagactcct attagacatc atgccagggc tggaaactgc
     1441 tgtcgtcttt caagaaaagg agggaattgt cgagaatctt ttcaaatggg cccgagaggc
     1501 cgatcaacca ttgaggacat attctactgg actgttagga ggtgctatgg aaaatcaaga
     1561 cattgctgcc aactatagag atgaaaattc acagctggtg gcaatagtgc ttcgaagact
     1621 gagggagcta cagctacagg aagtggcttt gcggcaggaa aacaagcgtc ccagtccacg
     1681 gaagctctct tctgaacccc ttttgcctct ggatgaggag gctgtggata tggactatgg
     1741 tgacatggct gtagatgtag tggatggaga ccaagaggaa gcttctggag acatggagat
     1801 ctcctttcat cttgattcag gccacaagac tagtagcaga gtgaactcaa caaccaaacc
     1861 tgaggatgga ggattaaaga aaaacaagtc agcaaaacag ggtgacagag agaactttag
     1921 gaaagccaag caaaagttgg gtttctcatc ttctgatcca gatcgcatgt ttgttgagct
     1981 gtctaatagc agttggtcag aaatgtctcc ctgggtgatt ggcaccaatt atacccttta
     2041 tcctatgact cctgctatcg agcagcgact cattctccaa tatttgaccc ctctaggaga
     2101 atatcaggag ctacttccca tattcatgca acttggatca cgggagctga tgatgttcta
     2161 tattgacctg aagcaaacta atgatgtcct gcttacattt gaggcactaa agcacctagc
     2221 atctctcctg ctccataaca aatttgccac agaatttgtt gcgcatggtg gagtacagaa
     2281 attactggaa atacctcgtc cttctatggc tgcaaccggt gtgtctatgt gtttgtatta
     2341 cctatcctac aatcaggatg ccatggaaag agtttgcatg catccccaca atgttctgtc
     2401 tgatgtggtg aactataccc tgtggttaat ggagtgttct catgcttcag gatgctgcca
     2461 tgctaccatg tttttttcaa tttgcttctc atttcgggcc gtcttggagc tctttgaccg
     2521 ctatgatggt cttcgtcgtc tggtgaactt gatcagtact ttggagattc taaatttgga
     2581 agatcagggt gcacttctga gtgatgatga aatatttgct agccgccaaa ctgggaaaca
     2641 tacctgcatg gccttgcgca aatactttga ggctcacctg gccattaaat tggaacaagt
     2701 gaagcagtca cttcagagga ctgagggtgg cattcttgtc cacccacaac ccccgtacaa
     2761 ggcatgctca tatactcatg aacagattgt ggaaatgatg gaatttttga tagaatatgg
     2821 cccagcgcag ctatattggg aaccagctga agttttcctc aaactttctt gtgtgcaact
     2881 cttgttgcag cttatttcta ttgcctgcaa ttggaagacc tattatgcaa ggaatgacac
     2941 tgtgcgcttt gctttggatg tcctggctat tcttactgtg gtgccaaaaa tccagctcca
     3001 gttggcagaa tcagtggacg tgttggatga ggctggatct acagtctcta ctgtaggtat
     3061 cagcattatt ttgggagtgg ctgagggtga gttcttcatc catgatgctg aaattcagaa
     3121 gtcagcactt cagattatca tcaattgtgt gtgtggccca gataaccgaa tatccagtat
     3181 tggtaaattt atctctggta ctcctcggag aaagctgcct cagaacccta aaagcagtga
     3241 gcacaccctg gccaagatgt ggaatgtggt tcagtccaac aacggcatca aggtgctcct
     3301 gtccttactg tccattaaga tgcccatcac agatgcagac caaatccggg ccctggcctg
     3361 caaagcccta gtgggcctgt ctcgcagtag cactgtccgg cagatcatca gtaaactgcc
     3421 ccttttcagc agctgccaga tccagcagct gatgaaggag cctgtgctgc aggacaagcg
     3481 cagtgaccat gtcaagttct gcaagtatgc tgctgaactc attgaacggg tgtcaggaaa
     3541 accacttctc attggcactg atgtttccct agcacgactg cagaaagcag atgttgttgc
     3601 ccagtcaagg atctccttcc ctgagaaaga gctgcttttg ttgatacgaa accatcttat
     3661 ttctaaaggg cttggagaaa cagcaaccgt gctgacaaaa gaggctgacc tgcccatgac
     3721 tgctgcctcc cattcttctg cctttacccc agtcactgct gctgcttctc ctgtctctct
     3781 accccgaacc cctcgtatcg ctaatggcat tgcaactcgt ctgggcagcc atgctgctgt
     3841 gggtgcctct gcgccttctg cccctactgc tcatcctcag ccacggcccc cccagggtcc
     3901 gctagctctg cccggcccat cttatgcagg caactcccct ttgattggta gaatcagttt
     3961 tatcagagag aggccatcac cctgcaatgg caggaaaatc agagtgttgc ggcagaagtc
     4021 ggaccatggt gcctacagcc aaagcccagc cataaaaaaa cagctggaca gacatcttcc
     4081 ttccccacct acgctggaca gtataatcac agagtatctt agagaacaac atgctcgctg
     4141 caagaatcca gttgccacct gcccaccttt ctccctcttt actcctcacc aatgtcctga
     4201 gccaaaacag aggcggcaag cgccaataaa ctttacgtca aggctaaacc gcagggcatc
     4261 atttccaaag tatggagggg tggatggcgg atgctttgat aggcacctta tctttagcag
     4321 attccgtcct atttcagtgt tccgggaagc caatgaagat gagagtggct tcacctgctg
     4381 tgcattctca gcacgggagc ggttcctgat gcttggcacc tgcacagggc agctgaagct
     4441 ctataatgtg tttagtggac aggaggaggc cagctataac tgtcacaact cagccatcac
     4501 acatcttgaa ccttccaggg atgggtcctt gctgctgaca tctgctactt ggagccagcc
     4561 tttgtctgca ctttggggaa tgaagtcagt atttgatatg aagcattcct tcacagaaga
     4621 tcactatgtt gagttcagta agcactccca ggatcgggtc atcggcacaa aaggagacat
     4681 tgcccacatt tatgatattc agactggcaa caagctgttg actctgttta acccagatct
     4741 tgccaacaac tacaagagga actgtgccac ctttaatcct acagatgatc ttgtcttaaa
     4801 tgatggcgtc ctctgggatg tccgctctgc acaggccatc cacaagtttg acaagttcaa
     4861 tatgaacatc agtggtgttt tccatccaaa tggactggag gtgatcatta atactgagat
     4921 ttgggacctt cgaacttttc atcttttgca tactgttccc gctctggatc agtgtcgcgt
     4981 ggtgttcaat cacacgggaa cagtgatgta tggagctatg ttgcaggcag atgatgaaga
     5041 tgacttaatg gaagagagga tgaaaagccc ctttgggtca tccttccgaa catttaatgc
     5101 aactgactac aaacctatag caaccattga tgtgaaacgg aacatctttg acctgtgtac
     5161 agacaccaaa gactgctatc ttgctgtcat tgagaatcaa ggcagcatgg atgccctgaa
     5221 catggacaca gtatgcaggc tgtatgaagt gggcaggcag cgtctggcag aggatgagga
     5281 tgaagaggag gaccaggaag aggaagaaca ggaggaagaa gatgatgatg aagatgatga
     5341 tgacaccgat gatttagatg agcttgacac tgaccagttg ctggaggcgg agttggagga
     5401 ggacgacaat aatgagaacg caggggaaga tggggacaat gacttctctc cctctgatga
     5461 ggagctagca aaccttctag aggagggaga ggacggggag gatgaagact ctgatgcaga
     5521 tgaggaggtg gaactgatcc tgggggacac tgacagctct gacaactctg atttggaaga
     5581 tgacatcatc ttatctctga atgagtctag atttgggccc aattcctgca ggcgagctct
     5641 cggctcgagc ggcgtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
     5701 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
     5761 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
     5821 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
     5881 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
     5941 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
     6001 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     6061 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
     6121 caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaaggcc gcgactctag
     6181 ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     6241 accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag acatgataag
     6301 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6361 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6421 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     6481 aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta
     6541 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     6601 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     6661 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6721 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6841 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7021 aagctcgatt cttctgacac tagcgccacc atgaagaagc ccgaactcac cgctaccagc
     7081 gttgaaaaat ttctcatcga gaagttcgac agtgtgagcg acctgatgca gttgtcggag
     7141 ggcgaagaga gccgagcctt cagcttcgat gtcggcggac gcggctatgt actgcgggtg
     7201 aatagctgcg ctgatggctt ctacaaagac cgctacgtgt accgccactt cgccagcgct
     7261 gcactaccca tccccgaagt gttggacatc ggcgagttca gcgagagcct gacatactgc
     7321 atcagtagac gcgcccaagg cgttactctc caagacctcc ccgaaacaga gctgcctgct
     7381 gtgttacagc ctgtcgccga agctatggat gctattgccg ccgccgacct cagtcaaacc
     7441 agcggcttcg gcccattcgg gccccaaggc atcggccagt acacaacctg gcgggatttc
     7501 atttgcgcca ttgctgatcc ccatgtctac cactggcaga ccgtgatgga cgacaccgtg
     7561 tccgccagcg tagctcaagc cctggacgaa ctgatgctgt gggccgaaga ctgtcccgag
     7621 gtgcgccacc tcgtccatgc cgacttcggc agcaacaacg tcctgaccga caacggccgc
     7681 atcaccgccg taatcgactg gtccgaagct atgttcgggg acagtcagta cgaggtggcc
     7741 aacatcttct tctggcggcc ctggctggct tgcatggagc agcagactcg ctacttcgag
     7801 cgccggcatc ccgagctggc cggcagccct cgtctgcgag cctacatgct gcgcatcggc
     7861 ctggatcagc tctaccagag cctcgtggac ggcaacttcg acgatgctgc ctgggctcaa
     7921 ggccgctgcg atgccatcgt ccgcagcggg gccggcaccg tcggtcgcac acaaatcgct
     7981 cgccggagcg cagccgtatg gaccgacggc tgcgtcgagg tgctggccga cagcggcaac
     8041 cgccggccca gtacacgacc gcgcgctaag gaggtaggtc gagtttagac tctagccatc
     8101 acgatggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     8161 aatcgatagc gataaggatc ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
     8221 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtaatggttt
     8281 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8341 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8401 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8461 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8521 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8581 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttc aaagttctgc
     8641 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8701 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8761 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8821 acttacttct gacaactatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8881 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8941 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9001 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9061 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9121 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9181 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9241 agatcgctga gataggtgcc tcactgatta agcattggta attcgaaatg accgaccaag
     9301 cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9841 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10021 atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata
    10141 gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga aaccatgaaa
    10201 aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg gttgcatagg
    10261 tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt ggtttgttgc
    10321 catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg cgtacaatta
    10381 agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagta
    10441 ctccggccac gatgcgtccg gcgtagagga tcgagatct
//
