LOCUS       sequence-423313-       10652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DCAF1. N-terminal 
            HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTN-HaloTag_DCAF1:1-1507
FEATURES             Location/Qualifiers
     source          1..10652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1067..1957
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1970..1990
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             complement(3226..3237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6603..6621)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6653..6774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6664..6683)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6718..6737
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7019..7376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7019..7210
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7042..7062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7227..7362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7427..8221
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7481..7500)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8091..8110
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8285..8333
                     /note="synthetic polyadenylation signal"
     promoter        8489..8593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8594..8662,8663..9454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8812..8831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9612..10200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9692..9711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10316..10599
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgcttccga attcatgact acagtagtgg tacatgtgga
     2041 ctccaaagct gagctcacta ccctgctgga gcagtgggaa aaggaacatg gcagtgggca
     2101 ggacatggta cctatcctta ccaggatgtc tcaattgatt gaaaaagaaa ctgaagagta
     2161 tcgtaaaggg gatccagacc catttgatga tcgacatcct ggtcgagctg atccagagtg
     2221 tatgctgggc cacttgctga gaatactctt caagaatgat gatttcatga atgcactggt
     2281 gaatgcatat gtgatgacaa gccgagagcc ccctttaaac actgcagctt gcagactcct
     2341 attagacatc atgccagggc tggaaactgc tgtcgtcttt caagaaaagg agggaattgt
     2401 cgagaatctt ttcaaatggg cccgagaggc cgatcaacca ttgaggacat attctactgg
     2461 actgttagga ggtgctatgg aaaatcaaga cattgctgcc aactatagag atgaaaattc
     2521 acagctggtg gcaatagtgc ttcgaagact gagggagcta cagctacagg aagtggcttt
     2581 gcggcaggaa aacaagcgtc ccagtccacg gaagctctct tctgaacccc ttttgcctct
     2641 ggatgaggag gctgtggata tggactatgg tgacatggct gtagatgtag tggatggaga
     2701 ccaagaggaa gcttctggag acatggagat ctcctttcat cttgattcag gccacaagac
     2761 tagtagcaga gtgaactcaa caaccaaacc tgaggatgga ggattaaaga aaaacaagtc
     2821 agcaaaacag ggtgacagag agaactttag gaaagccaag caaaagttgg gtttctcatc
     2881 ttctgatcca gatcgcatgt ttgttgagct gtctaatagc agttggtcag aaatgtctcc
     2941 ctgggtgatt ggcaccaatt atacccttta tcctatgact cctgctatcg agcagcgact
     3001 cattctccaa tatttgaccc ctctaggaga atatcaggag ctacttccca tattcatgca
     3061 acttggatca cgggagctga tgatgttcta tattgacctg aagcaaacta atgatgtcct
     3121 gcttacattt gaggcactaa agcacctagc atctctcctg ctccataaca aatttgccac
     3181 agaatttgtt gcgcatggtg gagtacagaa attactggaa atacctcgtc cttctatggc
     3241 tgcaaccggt gtgtctatgt gtttgtatta cctatcctac aatcaggatg ccatggaaag
     3301 agtttgcatg catccccaca atgttctgtc tgatgtggtg aactataccc tgtggttaat
     3361 ggagtgttct catgcttcag gatgctgcca tgctaccatg tttttttcaa tttgcttctc
     3421 atttcgggcc gtcttggagc tctttgaccg ctatgatggt cttcgtcgtc tggtgaactt
     3481 gatcagtact ttggagattc taaatttgga agatcagggt gcacttctga gtgatgatga
     3541 aatatttgct agccgccaaa ctgggaaaca tacctgcatg gccttgcgca aatactttga
     3601 ggctcacctg gccattaaat tggaacaagt gaagcagtca cttcagagga ctgagggtgg
     3661 cattcttgtc cacccacaac ccccgtacaa ggcatgctca tatactcatg aacagattgt
     3721 ggaaatgatg gaatttttga tagaatatgg cccagcgcag ctatattggg aaccagctga
     3781 agttttcctc aaactttctt gtgtgcaact cttgttgcag cttatttcta ttgcctgcaa
     3841 ttggaagacc tattatgcaa ggaatgacac tgtgcgcttt gctttggatg tcctggctat
     3901 tcttactgtg gtgccaaaaa tccagctcca gttggcagaa tcagtggacg tgttggatga
     3961 ggctggatct acagtctcta ctgtaggtat cagcattatt ttgggagtgg ctgagggtga
     4021 gttcttcatc catgatgctg aaattcagaa gtcagcactt cagattatca tcaattgtgt
     4081 gtgtggccca gataaccgaa tatccagtat tggtaaattt atctctggta ctcctcggag
     4141 aaagctgcct cagaacccta aaagcagtga gcacaccctg gccaagatgt ggaatgtggt
     4201 tcagtccaac aacggcatca aggtgctcct gtccttactg tccattaaga tgcccatcac
     4261 agatgcagac caaatccggg ccctggcctg caaagcccta gtgggcctgt ctcgcagtag
     4321 cactgtccgg cagatcatca gtaaactgcc ccttttcagc agctgccaga tccagcagct
     4381 gatgaaggag cctgtgctgc aggacaagcg cagtgaccat gtcaagttct gcaagtatgc
     4441 tgctgaactc attgaacggg tgtcaggaaa accacttctc attggcactg atgtttccct
     4501 agcacgactg cagaaagcag atgttgttgc ccagtcaagg atctccttcc ctgagaaaga
     4561 gctgcttttg ttgatacgaa accatcttat ttctaaaggg cttggagaaa cagcaaccgt
     4621 gctgacaaaa gaggctgacc tgcccatgac tgctgcctcc cattcttctg cctttacccc
     4681 agtcactgct gctgcttctc ctgtctctct accccgaacc cctcgtatcg ctaatggcat
     4741 tgcaactcgt ctgggcagcc atgctgctgt gggtgcctct gcgccttctg cccctactgc
     4801 tcatcctcag ccacggcccc cccagggtcc gctagctctg cccggcccat cttatgcagg
     4861 caactcccct ttgattggta gaatcagttt tatcagagag aggccatcac cctgcaatgg
     4921 caggaaaatc agagtgttgc ggcagaagtc ggaccatggt gcctacagcc aaagcccagc
     4981 cataaaaaaa cagctggaca gacatcttcc ttccccacct acgctggaca gtataatcac
     5041 agagtatctt agagaacaac atgctcgctg caagaatcca gttgccacct gcccaccttt
     5101 ctccctcttt actcctcacc aatgtcctga gccaaaacag aggcggcaag cgccaataaa
     5161 ctttacgtca aggctaaacc gcagggcatc atttccaaag tatggagggg tggatggcgg
     5221 atgctttgat aggcacctta tctttagcag attccgtcct atttcagtgt tccgggaagc
     5281 caatgaagat gagagtggct tcacctgctg tgcattctca gcacgggagc ggttcctgat
     5341 gcttggcacc tgcacagggc agctgaagct ctataatgtg tttagtggac aggaggaggc
     5401 cagctataac tgtcacaact cagccatcac acatcttgaa ccttccaggg atgggtcctt
     5461 gctgctgaca tctgctactt ggagccagcc tttgtctgca ctttggggaa tgaagtcagt
     5521 atttgatatg aagcattcct tcacagaaga tcactatgtt gagttcagta agcactccca
     5581 ggatcgggtc atcggcacaa aaggagacat tgcccacatt tatgatattc agactggcaa
     5641 caagctgttg actctgttta acccagatct tgccaacaac tacaagagga actgtgccac
     5701 ctttaatcct acagatgatc ttgtcttaaa tgatggcgtc ctctgggatg tccgctctgc
     5761 acaggccatc cacaagtttg acaagttcaa tatgaacatc agtggtgttt tccatccaaa
     5821 tggactggag gtgatcatta atactgagat ttgggacctt cgaacttttc atcttttgca
     5881 tactgttccc gctctggatc agtgtcgcgt ggtgttcaat cacacgggaa cagtgatgta
     5941 tggagctatg ttgcaggcag atgatgaaga tgacttaatg gaagagagga tgaaaagccc
     6001 ctttgggtca tccttccgaa catttaatgc aactgactac aaacctatag caaccattga
     6061 tgtgaaacgg aacatctttg acctgtgtac agacaccaaa gactgctatc ttgctgtcat
     6121 tgagaatcaa ggcagcatgg atgccctgaa catggacaca gtatgcaggc tgtatgaagt
     6181 gggcaggcag cgtctggcag aggatgagga tgaagaggag gaccaggaag aggaagaaca
     6241 ggaggaagaa gatgatgatg aagatgatga tgacaccgat gatttagatg agcttgacac
     6301 tgaccagttg ctggaggcgg agttggagga ggacgacaat aatgagaacg caggggaaga
     6361 tggggacaat gacttctctc cctctgatga ggagctagca aaccttctag aggagggaga
     6421 ggacggggag gatgaagact ctgatgcaga tgaggaggtg gaactgatcc tgggggacac
     6481 tgacagctct gacaactctg atttggaaga tgacatcatc ttatctctga atgagtgatg
     6541 atctagattt gggcccaatt cctgcaggat tttgcggccg cttgctgagt tggctgctgc
     6601 caccgctgag caataactag cataacccct tggccgcttc gagcagacat gataagatac
     6661 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     6721 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     6781 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc
     6841 aagtaaaacc tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat
     6901 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga
     6961 tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct
     7021 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7201 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7381 ttgattcttc tgacacaaca gtctcgaact taaggctaga gccaccatga ttgaacaaga
     7441 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7501 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7561 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7621 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7681 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7741 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7801 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7861 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7921 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7981 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     8041 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     8101 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     8161 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     8221 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
     8281 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     8341 agcgataagg atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     8401 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtaatgg tttcttagac
     8461 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8581 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8641 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8701 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8761 gagttttcgc cccgaagaac gttttccaat gatgagcact ttcaaagttc tgctatgtgg
     8821 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8881 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8941 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9001 tctgacaact atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9061 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9121 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9181 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9241 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9301 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9361 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9421 tgagataggt gcctcactga ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc
     9481 caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9661 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9781 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9841 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9901 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9961 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10021 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    10081 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10141 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa
    10201 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10261 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga
    10321 aatacaggaa cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca
    10381 gcttcagtgg attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat
    10441 acggttaaaa tttatcaggc gcgattgcgg cagtttttcg ggtggtttgt tgccattttt
    10501 acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt
    10561 atggtaaatc cacttactgt ctgccctcgt agccatcgag ataaaccgca gtactccggc
    10621 cacgatgcgt ccggcgtaga ggatcgagat ct
//
