LOCUS       sequence-423314-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DCAF1. C-terminal 
            HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTC-HaloTag_DCAF1:1-1507
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2293..2304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5618..5638
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             5654..6538
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(6610..6628)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6665..6786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6676..6695)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6730..6749
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7031..7388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7031..7222
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7054..7074)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7239..7374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7439..8233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7493..7512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8103..8122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8297..8345
                     /note="synthetic polyadenylation signal"
     promoter        8501..8605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8606..8674,8675..9466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8824..8843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9624..10212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9704..9723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10328..10611
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcac
     1081 catgactaca gtagtggtac atgtggactc caaagctgag ctcactaccc tgctggagca
     1141 gtgggaaaag gaacatggca gtgggcagga catggtacct atccttacca ggatgtctca
     1201 attgattgaa aaagaaactg aagagtatcg taaaggggat ccagacccat ttgatgatcg
     1261 acatcctggt cgagctgatc cagagtgtat gctgggccac ttgctgagaa tactcttcaa
     1321 gaatgatgat ttcatgaatg cactggtgaa tgcatatgtg atgacaagcc gagagccccc
     1381 tttaaacact gcagcttgca gactcctatt agacatcatg ccagggctgg aaactgctgt
     1441 cgtctttcaa gaaaaggagg gaattgtcga gaatcttttc aaatgggccc gagaggccga
     1501 tcaaccattg aggacatatt ctactggact gttaggaggt gctatggaaa atcaagacat
     1561 tgctgccaac tatagagatg aaaattcaca gctggtggca atagtgcttc gaagactgag
     1621 ggagctacag ctacaggaag tggctttgcg gcaggaaaac aagcgtccca gtccacggaa
     1681 gctctcttct gaaccccttt tgcctctgga tgaggaggct gtggatatgg actatggtga
     1741 catggctgta gatgtagtgg atggagacca agaggaagct tctggagaca tggagatctc
     1801 ctttcatctt gattcaggcc acaagactag tagcagagtg aactcaacaa ccaaacctga
     1861 ggatggagga ttaaagaaaa acaagtcagc aaaacagggt gacagagaga actttaggaa
     1921 agccaagcaa aagttgggtt tctcatcttc tgatccagat cgcatgtttg ttgagctgtc
     1981 taatagcagt tggtcagaaa tgtctccctg ggtgattggc accaattata ccctttatcc
     2041 tatgactcct gctatcgagc agcgactcat tctccaatat ttgacccctc taggagaata
     2101 tcaggagcta cttcccatat tcatgcaact tggatcacgg gagctgatga tgttctatat
     2161 tgacctgaag caaactaatg atgtcctgct tacatttgag gcactaaagc acctagcatc
     2221 tctcctgctc cataacaaat ttgccacaga atttgttgcg catggtggag tacagaaatt
     2281 actggaaata cctcgtcctt ctatggctgc aaccggtgtg tctatgtgtt tgtattacct
     2341 atcctacaat caggatgcca tggaaagagt ttgcatgcat ccccacaatg ttctgtctga
     2401 tgtggtgaac tataccctgt ggttaatgga gtgttctcat gcttcaggat gctgccatgc
     2461 taccatgttt ttttcaattt gcttctcatt tcgggccgtc ttggagctct ttgaccgcta
     2521 tgatggtctt cgtcgtctgg tgaacttgat cagtactttg gagattctaa atttggaaga
     2581 tcagggtgca cttctgagtg atgatgaaat atttgctagc cgccaaactg ggaaacatac
     2641 ctgcatggcc ttgcgcaaat actttgaggc tcacctggcc attaaattgg aacaagtgaa
     2701 gcagtcactt cagaggactg agggtggcat tcttgtccac ccacaacccc cgtacaaggc
     2761 atgctcatat actcatgaac agattgtgga aatgatggaa tttttgatag aatatggccc
     2821 agcgcagcta tattgggaac cagctgaagt tttcctcaaa ctttcttgtg tgcaactctt
     2881 gttgcagctt atttctattg cctgcaattg gaagacctat tatgcaagga atgacactgt
     2941 gcgctttgct ttggatgtcc tggctattct tactgtggtg ccaaaaatcc agctccagtt
     3001 ggcagaatca gtggacgtgt tggatgaggc tggatctaca gtctctactg taggtatcag
     3061 cattattttg ggagtggctg agggtgagtt cttcatccat gatgctgaaa ttcagaagtc
     3121 agcacttcag attatcatca attgtgtgtg tggcccagat aaccgaatat ccagtattgg
     3181 taaatttatc tctggtactc ctcggagaaa gctgcctcag aaccctaaaa gcagtgagca
     3241 caccctggcc aagatgtgga atgtggttca gtccaacaac ggcatcaagg tgctcctgtc
     3301 cttactgtcc attaagatgc ccatcacaga tgcagaccaa atccgggccc tggcctgcaa
     3361 agccctagtg ggcctgtctc gcagtagcac tgtccggcag atcatcagta aactgcccct
     3421 tttcagcagc tgccagatcc agcagctgat gaaggagcct gtgctgcagg acaagcgcag
     3481 tgaccatgtc aagttctgca agtatgctgc tgaactcatt gaacgggtgt caggaaaacc
     3541 acttctcatt ggcactgatg tttccctagc acgactgcag aaagcagatg ttgttgccca
     3601 gtcaaggatc tccttccctg agaaagagct gcttttgttg atacgaaacc atcttatttc
     3661 taaagggctt ggagaaacag caaccgtgct gacaaaagag gctgacctgc ccatgactgc
     3721 tgcctcccat tcttctgcct ttaccccagt cactgctgct gcttctcctg tctctctacc
     3781 ccgaacccct cgtatcgcta atggcattgc aactcgtctg ggcagccatg ctgctgtggg
     3841 tgcctctgcg ccttctgccc ctactgctca tcctcagcca cggccccccc agggtccgct
     3901 agctctgccc ggcccatctt atgcaggcaa ctcccctttg attggtagaa tcagttttat
     3961 cagagagagg ccatcaccct gcaatggcag gaaaatcaga gtgttgcggc agaagtcgga
     4021 ccatggtgcc tacagccaaa gcccagccat aaaaaaacag ctggacagac atcttccttc
     4081 cccacctacg ctggacagta taatcacaga gtatcttaga gaacaacatg ctcgctgcaa
     4141 gaatccagtt gccacctgcc cacctttctc cctctttact cctcaccaat gtcctgagcc
     4201 aaaacagagg cggcaagcgc caataaactt tacgtcaagg ctaaaccgca gggcatcatt
     4261 tccaaagtat ggaggggtgg atggcggatg ctttgatagg caccttatct ttagcagatt
     4321 ccgtcctatt tcagtgttcc gggaagccaa tgaagatgag agtggcttca cctgctgtgc
     4381 attctcagca cgggagcggt tcctgatgct tggcacctgc acagggcagc tgaagctcta
     4441 taatgtgttt agtggacagg aggaggccag ctataactgt cacaactcag ccatcacaca
     4501 tcttgaacct tccagggatg ggtccttgct gctgacatct gctacttgga gccagccttt
     4561 gtctgcactt tggggaatga agtcagtatt tgatatgaag cattccttca cagaagatca
     4621 ctatgttgag ttcagtaagc actcccagga tcgggtcatc ggcacaaaag gagacattgc
     4681 ccacatttat gatattcaga ctggcaacaa gctgttgact ctgtttaacc cagatcttgc
     4741 caacaactac aagaggaact gtgccacctt taatcctaca gatgatcttg tcttaaatga
     4801 tggcgtcctc tgggatgtcc gctctgcaca ggccatccac aagtttgaca agttcaatat
     4861 gaacatcagt ggtgttttcc atccaaatgg actggaggtg atcattaata ctgagatttg
     4921 ggaccttcga acttttcatc ttttgcatac tgttcccgct ctggatcagt gtcgcgtggt
     4981 gttcaatcac acgggaacag tgatgtatgg agctatgttg caggcagatg atgaagatga
     5041 cttaatggaa gagaggatga aaagcccctt tgggtcatcc ttccgaacat ttaatgcaac
     5101 tgactacaaa cctatagcaa ccattgatgt gaaacggaac atctttgacc tgtgtacaga
     5161 caccaaagac tgctatcttg ctgtcattga gaatcaaggc agcatggatg ccctgaacat
     5221 ggacacagta tgcaggctgt atgaagtggg caggcagcgt ctggcagagg atgaggatga
     5281 agaggaggac caggaagagg aagaacagga ggaagaagat gatgatgaag atgatgatga
     5341 caccgatgat ttagatgagc ttgacactga ccagttgctg gaggcggagt tggaggagga
     5401 cgacaataat gagaacgcag gggaagatgg ggacaatgac ttctctccct ctgatgagga
     5461 gctagcaaac cttctagagg agggagagga cggggaggat gaagactctg atgcagatga
     5521 ggaggtggaa ctgatcctgg gggacactga cagctctgac aactctgatt tggaagatga
     5581 catcatctta tctctgaatg agctcgagcc aaccactgag gatctgtact ttcagagcga
     5641 taacgatgga tccgaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct
     5701 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
     5761 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
     5821 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
     5881 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
     5941 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
     6001 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
     6061 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
     6121 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
     6181 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
     6241 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
     6301 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
     6361 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
     6421 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
     6481 cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga tttccggtta
     6541 atagaattgg catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     6601 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac
     6661 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     6721 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     6781 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     6841 gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt
     6901 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     6961 cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     7021 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     7081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7141 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7201 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7261 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7321 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7381 tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat
     7441 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7501 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7561 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7621 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7681 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7741 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7801 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7861 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7921 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7981 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8041 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8101 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8161 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8221 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8281 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     8341 gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc
     8401 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtaat
     8461 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca ctttcaaagt
     8821 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9001 ggccaactta cttctgacaa ctatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9121 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9301 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaattcg aaatgaccga
     9481 ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    10081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10201 aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10321 ttatagtccg gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca
    10381 tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc
    10441 ataggtattc atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt
    10501 gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac
    10561 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
    10621 cagtactccg gccacgatgc gtccggcgta gaggatcgag atct
//
