LOCUS       sequence-423323-        8942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human SMCR8. N-terminal 
            HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTN-HaloTag_SMCR8:1-937
FEATURES             Location/Qualifiers
     source          1..8942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1067..1957
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1970..1990
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(4893..4911)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4943..5064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4954..4973)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5008..5027
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5309..5666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5309..5500
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5332..5352)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5517..5652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5717..6511
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5771..5790)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6381..6400
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6575..6623
                     /note="synthetic polyadenylation signal"
     promoter        6779..6883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6884..6952,6953..7744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7102..7121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7902..8490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7982..8001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8606..8889
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgcttccga attcatgatc agcgcccctg acgtagtggc
     2041 cttcaccaaa gaggaagagt atgaagaaga gccttacaat gagccggccc tgcctgagga
     2101 gtactcggtg ccgctcttcc ccttcgccag tcagggtgct aacccctggt caaaactgtc
     2161 cggggccaag ttttcgaggg acttcattct tatttccgag ttctctgagc aggtgggacc
     2221 ccaaccctta ctgaccatcc ccaatgacac caaagttttt ggcacttttg atctcaatta
     2281 cttctccctg cgtatcatgt ctgtggatta ccaggcttcc ttcgtgggcc atcctcctgg
     2341 atctgcctac cccaagctga acttcgtgga ggactccaag gtggtgctgg gagattctaa
     2401 ggagggcgcc tttgcatacg tgcaccacct taccctatac gacctggagg cccgtggctt
     2461 cgtgaggccg ttttgcatgg cttatatctc tgcagaccag cataaaatca tgcagcagtt
     2521 ccaggagctt tcagccgaat tttccagagc ttctgagtgc ttgaagactg gcaacaggaa
     2581 ggcatttgct ggggaacttg aaaaaaagct gaaagacttg gattacacca ggacagtgct
     2641 acacacagaa acggagatcc agaagaaagc caacgacaaa ggcttttact catctcaggc
     2701 aattgagaaa gccaatgaac tggccagtgt ggagaagtcc atcattgaac atcaagacct
     2761 gctgaagcag atccgctcat accctcatcg gaagttgaag gggcatgatt tgtgtcctgg
     2821 tgagatggag cacatccagg atcaggccag ccaggcatcc actacctcta accctgatga
     2881 gtctgccgac acagaccttt acacctgcag accagcctac accccaaaac ttatcaaagc
     2941 aaagtccacc aagtgttttg acaagaagtt gaagaccttg gaggagctct gtgacactga
     3001 atatttcacc cagaccctgg ctcagctcag ccacattgaa cacatgttca gaggagacct
     3061 gtgttacctc ctgaccagtc agattgatag agcacttcta aaacaacagc atataacaaa
     3121 ctttctcttt gaagactttg tggaggtcga tgacaggatg gtggagaaac aagaaagcat
     3181 accctctaag cccagtcaag acaggccgcc ttccagttct ctagaagaat gcccaattcc
     3241 taaagtgtta attagtgttg gttcttacaa gtccagtgtg gagtctgtgt tgatcaagat
     3301 ggagcaggaa ctgggagatg aggagtacaa ggaagtggaa gtgactgagt tgagcagttt
     3361 cgacccccag gaaaacttgg actacctgga tatggatatg aaagggagta tcagcagtgg
     3421 tgaaagtatt gaagttttgg gcacggagaa atccacctcc gtgctttcta aatctgacag
     3481 ccaggcaagc ctcacagtac cattgagccc ccaggtggtc cggagcaaag cagtcagcca
     3541 caggaccatc agcgaggaca gtattgaagt cctcagtacc tgcccctctg aggccctcat
     3601 ccctgatgac tttaaggcca gctacccaag tgccattaat gaagaagaat catatccaga
     3661 tggcaatgaa ggagccatcc gcttccaggc aagcatcagt cctccagaac tgggtgagac
     3721 agaggaaggc agcatagaaa acaccccatc acaaatagac tcctcctgct gtattgggaa
     3781 ggagagcgat ggtcagttgg tgctgccctc cactccagcc cacacacact ctgacgagga
     3841 tggggtggtg agcagccccc cacagcgcca caggcagaag gaccaggggt tccgtgtaga
     3901 cttttcagtg gaaaatgcca acccttcttc ccgagacaac agttgtgaag ggtttcccgc
     3961 ttatgagctg gacccgagcc acctgctggc tagccgggac atcagtaaga ccagcctgga
     4021 caactactca gacaccacca gctacgtgag cagtgtagcg tccaccagct cagacaggat
     4081 cccctctgct tatcctgctg gcctgtcttc cgataggcat aaaaagaggg ctggccagaa
     4141 cgccttaaaa ttcatccgcc agtacccctt tgcccaccca gccatctact ccctgctcag
     4201 tgggaggaca cttgtggtcc tgggggaaga tgaggccata gtcaggaaac tcgtgactgc
     4261 actggctatc tttgtcccca gctatggctg ctacgctaag cccgtgaaac attgggcctc
     4321 ctcccctttg cacattatgg attttcagaa gtggaagctt attggcttgc agagagtggc
     4381 ctcccctgcc ggtgccggta ccctccatgc cctgagccgc tacagccgct acacgagcat
     4441 cctggacctt gacaacaaaa ccctgcgctg ccccctttac agaggcaccc tggtgccccg
     4501 cctggcagac caccgcacac agatcaagcg gggcagcacc tactacctgc atgtccagag
     4561 catgctcacc cagctctgct ccaaggcctt cctctacacc ttctgccacc acctgcacct
     4621 gcctacccac gacaaggaga cagaggagct ggtagccagc cgccagatga gcttcctaaa
     4681 gctgaccctg ggtctggtga atgaggatgt tagggtggtc cagtacctgg ctgagctgct
     4741 gaagctgcac tacatgcagg aatctccagg gaccagccac cccatgctca ggtttgacta
     4801 tgtccccagc tttttgtata aaatctgatg atctagattt gggcccaatt cctgcaggat
     4861 tttgcggccg cttgctgagt tggctgctgc caccgctgag caataactag cataacccct
     4921 tggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4981 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     5041 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     5101 cagggggaga tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc
     5161 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     5221 ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct
     5281 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
     5341 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5401 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5461 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5521 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5581 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5641 tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact
     5701 taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5761 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5821 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5881 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5941 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6001 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6061 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6121 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6181 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6241 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6301 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6361 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6421 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6481 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6541 caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca
     6601 tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atcctctttg cgcttgcgtt
     6661 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     6721 actggctttc tacgtaatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7081 gatgagcact ttcaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7261 catgagtgat aacactgcgg ccaacttact tctgacaact atcggaggac cgaaggagct
     7321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7741 gtaattcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc aaaggcggta
     7801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8341 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8461 cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc tacggggtct
     8521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8581 atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg atggcccttc
     8641 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     8701 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgattgcgg
     8761 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg
     8821 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt
     8881 agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga ggatcgagat
     8941 ct
//
