LOCUS       sequence-423324-        8954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human SMCR8. C-terminal 
            HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTC-HaloTag_SMCR8:1-937
FEATURES             Location/Qualifiers
     source          1..8954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3908..3928
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             3944..4828
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(4900..4918)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4955..5076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4966..4985)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5020..5039
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5321..5678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5321..5512
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5344..5364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5529..5664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5729..6523
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5783..5802)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6393..6412
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6587..6635
                     /note="synthetic polyadenylation signal"
     promoter        6791..6895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6896..6964,6965..7756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7114..7133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7914..8502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7994..8013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8618..8901
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcac
     1081 catgatcagc gcccctgacg tagtggcctt caccaaagag gaagagtatg aagaagagcc
     1141 ttacaatgag ccggccctgc ctgaggagta ctcggtgccg ctcttcccct tcgccagtca
     1201 gggtgctaac ccctggtcaa aactgtccgg ggccaagttt tcgagggact tcattcttat
     1261 ttccgagttc tctgagcagg tgggacccca acccttactg accatcccca atgacaccaa
     1321 agtttttggc acttttgatc tcaattactt ctccctgcgt atcatgtctg tggattacca
     1381 ggcttccttc gtgggccatc ctcctggatc tgcctacccc aagctgaact tcgtggagga
     1441 ctccaaggtg gtgctgggag attctaagga gggcgccttt gcatacgtgc accaccttac
     1501 cctatacgac ctggaggccc gtggcttcgt gaggccgttt tgcatggctt atatctctgc
     1561 agaccagcat aaaatcatgc agcagttcca ggagctttca gccgaatttt ccagagcttc
     1621 tgagtgcttg aagactggca acaggaaggc atttgctggg gaacttgaaa aaaagctgaa
     1681 agacttggat tacaccagga cagtgctaca cacagaaacg gagatccaga agaaagccaa
     1741 cgacaaaggc ttttactcat ctcaggcaat tgagaaagcc aatgaactgg ccagtgtgga
     1801 gaagtccatc attgaacatc aagacctgct gaagcagatc cgctcatacc ctcatcggaa
     1861 gttgaagggg catgatttgt gtcctggtga gatggagcac atccaggatc aggccagcca
     1921 ggcatccact acctctaacc ctgatgagtc tgccgacaca gacctttaca cctgcagacc
     1981 agcctacacc ccaaaactta tcaaagcaaa gtccaccaag tgttttgaca agaagttgaa
     2041 gaccttggag gagctctgtg acactgaata tttcacccag accctggctc agctcagcca
     2101 cattgaacac atgttcagag gagacctgtg ttacctcctg accagtcaga ttgatagagc
     2161 acttctaaaa caacagcata taacaaactt tctctttgaa gactttgtgg aggtcgatga
     2221 caggatggtg gagaaacaag aaagcatacc ctctaagccc agtcaagaca ggccgccttc
     2281 cagttctcta gaagaatgcc caattcctaa agtgttaatt agtgttggtt cttacaagtc
     2341 cagtgtggag tctgtgttga tcaagatgga gcaggaactg ggagatgagg agtacaagga
     2401 agtggaagtg actgagttga gcagtttcga cccccaggaa aacttggact acctggatat
     2461 ggatatgaaa gggagtatca gcagtggtga aagtattgaa gttttgggca cggagaaatc
     2521 cacctccgtg ctttctaaat ctgacagcca ggcaagcctc acagtaccat tgagccccca
     2581 ggtggtccgg agcaaagcag tcagccacag gaccatcagc gaggacagta ttgaagtcct
     2641 cagtacctgc ccctctgagg ccctcatccc tgatgacttt aaggccagct acccaagtgc
     2701 cattaatgaa gaagaatcat atccagatgg caatgaagga gccatccgct tccaggcaag
     2761 catcagtcct ccagaactgg gtgagacaga ggaaggcagc atagaaaaca ccccatcaca
     2821 aatagactcc tcctgctgta ttgggaagga gagcgatggt cagttggtgc tgccctccac
     2881 tccagcccac acacactctg acgaggatgg ggtggtgagc agccccccac agcgccacag
     2941 gcagaaggac caggggttcc gtgtagactt ttcagtggaa aatgccaacc cttcttcccg
     3001 agacaacagt tgtgaagggt ttcccgctta tgagctggac ccgagccacc tgctggctag
     3061 ccgggacatc agtaagacca gcctggacaa ctactcagac accaccagct acgtgagcag
     3121 tgtagcgtcc accagctcag acaggatccc ctctgcttat cctgctggcc tgtcttccga
     3181 taggcataaa aagagggctg gccagaacgc cttaaaattc atccgccagt acccctttgc
     3241 ccacccagcc atctactccc tgctcagtgg gaggacactt gtggtcctgg gggaagatga
     3301 ggccatagtc aggaaactcg tgactgcact ggctatcttt gtccccagct atggctgcta
     3361 cgctaagccc gtgaaacatt gggcctcctc ccctttgcac attatggatt ttcagaagtg
     3421 gaagcttatt ggcttgcaga gagtggcctc ccctgccggt gccggtaccc tccatgccct
     3481 gagccgctac agccgctaca cgagcatcct ggaccttgac aacaaaaccc tgcgctgccc
     3541 cctttacaga ggcaccctgg tgccccgcct ggcagaccac cgcacacaga tcaagcgggg
     3601 cagcacctac tacctgcatg tccagagcat gctcacccag ctctgctcca aggccttcct
     3661 ctacaccttc tgccaccacc tgcacctgcc tacccacgac aaggagacag aggagctggt
     3721 agccagccgc cagatgagct tcctaaagct gaccctgggt ctggtgaatg aggatgttag
     3781 ggtggtccag tacctggctg agctgctgaa gctgcactac atgcaggaat ctccagggac
     3841 cagccacccc atgctcaggt ttgactatgt ccccagcttt ttgtataaaa tcctcgagcc
     3901 aaccactgag gatctgtact ttcagagcga taacgatgga tccgaaatcg gtactggctt
     3961 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     4021 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     4081 gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     4141 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     4201 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     4261 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     4321 tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg cccgcgagac
     4381 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     4441 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     4501 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     4561 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     4621 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     4681 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     4741 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     4801 ctggctgtct actctggaga tttccggtta atagaattgg catgcaagct gatccggctg
     4861 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     4921 aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     4981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     5101 atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa
     5161 tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     5221 ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc
     5281 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     5341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct tctgacacaa
     5701 cagtctcgaa cttaaggcta gagccaccat gattgaacaa gatggattgc acgcaggttc
     5761 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5821 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5881 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5941 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6001 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6061 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6121 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6181 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6241 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6301 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6361 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6421 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6481 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6541 gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt
     6601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcctctt
     6661 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     6721 ccgtttctgc ggactggctt tctacgtaat ggtttcttag acgtcaggtg gcacttttcg
     6781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7081 acgttttcca atgatgagca ctttcaaagt tctgctatgt ggcgcggtat tatcccgtat
     7141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa ctatcggagg
     7321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7741 gattaagcat tggtaattcg aaatgaccga ccaagcgacg cccaaccggt atcagctcac
     7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt
     8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8581 ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg aacgcacgct
     8641 ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg
     8701 ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa aatttatcag
     8761 gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga
     8821 tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tccacttact
     8881 gtctgccctc gtagccatcg agataaaccg cagtactccg gccacgatgc gtccggcgta
     8941 gaggatcgag atct
//
