LOCUS       sequence-423330-       11954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR81. N-terminal 
            HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 11954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTN-HaloTag_WDR81:1-1941
FEATURES             Location/Qualifiers
     source          1..11954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1067..1957
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1970..1990
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(7905..7923)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7955..8076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7966..7985)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8020..8039
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8321..8678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8321..8512
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8344..8364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8529..8664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8729..9523
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8783..8802)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9393..9412
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9587..9635
                     /note="synthetic polyadenylation signal"
     promoter        9791..9895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9896..9964,9965..10756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10114..10133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10914..11502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10994..11013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11618..11901
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgcttccga attcatggca cagggctctg ggggtcgaga
     2041 aggtgcactc cggacgcctg ctggtggatg gcacagcccg ccttcccctg acatgcagga
     2101 attgcttcgc tccgtagaaa gggatctctc tatagatcca cgccagcttg cgccggcacc
     2161 tggaggtacg catgtcgtag ccctggtacc ggccaggtgg ctcgccagtc tgagagatag
     2221 acgactccca ttgggacctt gccccagggc tgagggactt ggtgaggcgg aggtgcgaac
     2281 cttgctgcag aggtctgtcc agaggcttcc tgcgggttgg acaagggtag aggtgcacgg
     2341 tctccgaaaa cggcggttgt cctatccgct gggtggtggc ctcccttttg aggacggaag
     2401 ttgcggccct gaaactctca cccggtttat gcaagaagta gccgcacaaa actaccgaaa
     2461 tctgtggagg catgcttatc atacgtacgg gcaaccgtat tcccattccc ctgcgccgag
     2521 cgcagtaccc gcactggata gtgtgcgaca agctcttcag cgggtttatg gttgttcttt
     2581 tttgcccgtt ggcgaaacga cgcaatgccc ctcttacgct cgagagggac cttgtcctcc
     2641 acgagggagc cctgcctgtc cttccttgtt gcgggcagag gctctcctcg agagtccgga
     2701 gatgctgtat gttgtgcacc cttacgtgca gtttagcctg catgatgtgg tgaccttcag
     2761 cccagcaaag ttgactaata gtcaggcaaa agtgctcttt atcttgtttc gagtcctgcg
     2821 agccatggac gcttgccacc ggcaaggtct cgcctgcggt gcattgtctc tttaccatat
     2881 tgccgtcgat gagaagctct gcagtgagct tagattggat ctgagtgctt acgaacggcc
     2941 tgaggaggac gagaatgagg aggccccagt ggcgcgagac gaagcgggta tcgtatccca
     3001 agaggagcag ggtggccaac ccggtcaacc cacgggtcaa gaagagctta gatctctcgt
     3061 gcttgactgg gtccacggca gaatctccaa cttccactat ttgatgcaac tcaacagact
     3121 ggcggggcgg aggcagggtg acccgaatta ccatccagtc ctgccgtggg tggtagattt
     3181 cacaacccca catggtcggt tccgagacct ccggaaatct aagttcaggt tgaacaaggg
     3241 cgataagcaa ctggacttta cctatgaaat gactcggcag gcctttgtag cgggcggagc
     3301 agggggggga gagcctcccc acgtgccgca tcatatctca gatgtcctgt ccgacatcac
     3361 ctactatgtg tacaaagcca gaaggactcc tcggtctgtt ttgtgtggac acgttcgcgc
     3421 ccaatgggaa ccccatgaat atccagcgtc aatggagcga atgcagaatt ggacgccaga
     3481 cgaatgtatt cctgagttct acaccgaccc gagtatattt cgatctattc accccgatat
     3541 gcctgacttg gatgtgcccg catggtgctc aagtagccag gagttcgtag ctgcccatcg
     3601 cgccttgttg gaatcacggg aagtaagccg ggatctgcac cattggattg accttacatt
     3661 cggatacaaa ttgcagggta aagaggcagt taaggaaaag aacgtttgtc ttcatctggt
     3721 tgacgcacac acgcatcttg cgtcctacgg ggttgtccaa ttgttcgatc aaccccatcc
     3781 gcaaaggctc gctggcgccc cagctcttgc cccagaaccg ccgcttatac ctaaacttct
     3841 ggtgcagacg atacaagaaa ccactggtcg agaggatttt accgaaaatc ctgggcaatt
     3901 gcccaacgga gtcggcagac cagtattgga agcaaccccc tgtgaagctt cttggactag
     3961 ggatagacct gtggccggtg aagatgacct ggagcaagcc accgaagcac tggatagtat
     4021 aagcctcgcg ggaaaggccg gggaccagct cggaagcagc tctcaagcca gtcctggcct
     4081 cctcagcttt tcagtcgcga gtgcctcaag acctgggcgg cgcaacaaag ccgccggtgc
     4141 cgacccaggc gaaggggagg aagggaggat attgcttcca gaagggttca atcctatgca
     4201 ggccttggag gagctggaaa aaacaggaaa cttccttgct aagggccttg gcggcctgct
     4261 ggaggtccca gagcagccta gggtacagcc cgcggtaccc ttgcagtgcc tgttgcaccg
     4321 agacatgcag gccttgggtg tcctcctcgc ggaaatggtg tttgcgacgc gagtccgaac
     4381 gctccaacct gacgctcctt tgtgggtgcg ctttcaggca gtgcgcggac tttgtacacg
     4441 gcacccgaag gaggtgccag tttcccttca gcctgtattg gatacattgt tgcagatgtc
     4501 cggaccggag gttcccatgg gtgccgagcg gggtaagctt gaccaattgt tcgagtatcg
     4561 accggtgagt caaggacttc cacccccatg cccatcacag ctgctgtccc cctttagctc
     4621 agtagttccc ttccctcctt atttcccggc actgcatagg tttattctgc tttatcaggc
     4681 tcgacgcgtc gaggatgagg cccaaggccg ggagttggta ttcgccctct ggcaacagct
     4741 gggcgctgtg cttaaagaca ttaccccaga gggacttgag atattgctgc cctttgtcct
     4801 cagccttatg tccgaggaac ataccgcggt gtatacagcc tggtacctct ttgagcctgt
     4861 tgcgaaggct ctgggaccta agaacgcaaa taagtacctc ctgaaacccc tcataggagc
     4921 ttatgagtcc ccttgccaat tgcacgggag gttttatttg tatacagatt gttttgtggc
     4981 ccagctcatg gtaagattgg gcctgcaggc ctttttgacg cacctgttgc cgcacgtact
     5041 ccaagtattg gccggagctg aagcatcaca ggaagagtca aaggatctgg cgggagctgc
     5101 tgaagaagaa gagtccggtc ttccaggggc gggcccaggc agttgcgcat ttggagagga
     5161 aatcccgatg gatggcgaac ctccagcgtc ttcaggtctc ggactcccag actatacaag
     5221 cggggttagc ttccacgatc aggctgacct ccccgagacc gaagatttcc aagctggtct
     5281 gtatgtaacc gaaagtccac aaccgcaaga agcggaggca gtgtccttgg gtcgactcag
     5341 cgacaaatct tctacatccg aaacttctct gggcgaggag agggcaccgg atgaaggagg
     5401 ggcccctgtt gacaaaagct cacttaggtc cggagatagt agtcaagatc tcaaacagtc
     5461 tgaaggatcc gaagaagaag aggaggaaga ggattcctgt gttgtgctgg aggaggaaga
     5521 gggagaacag gaggaagtta ccggcgcaag tgaactgaca cttagtgata cggtgctctc
     5581 aatggagacc gtagtcgccg gtggctcagg aggagacggg gaagaggaag aggaggccct
     5641 gccagaacaa tcagaaggta aagaacaaaa gatcctcctt gacaccgcct gtaagatggt
     5701 ccgctggctc tccgctaagt tgggaccaac ggttgcgagt cggcacgtgg cgcggaatct
     5761 cctgagactg ctcacaagct gctacgttgg ccctactagg caacagttca cagtctcctc
     5821 tggagaatcc cccccactct ccgctggaaa catctaccag aagagaccag tactgggtga
     5881 catcgtctct gggccagtgt tgagctgctt gttgcacatc gcacgattgt atggcgaacc
     5941 ggtacttacg tatcaatacc tgccatatat ttcttacctc gtggcccccg gctcagcttc
     6001 tgggccctcc cgcctcaata gccgaaaaga agcgggtctg cttgctgctg tcacgttgac
     6061 tcaaaaaatt attgtatacc ttagcgacac gacactgatg gacatcttgc caagaatcag
     6121 ccatgaggtg ctgcttccag tcttgtcatt tttgaccagt cttgttacgg ggtttccgtc
     6181 cggtgcccaa gcaaggacta tcctttgtgt aaaaacaata tcattgatag cactcatttg
     6241 ccttaggatt ggacaggaga tggtacaaca acatttgtcc gaacccgtag ctactttttt
     6301 ccaagtcttt agtcagctgc acgagctccg ccagcaggac ttgaagttgg atcctgctgg
     6361 taggggagag ggccaactgc cccaggtcgt tttcagcgat gggcaacaaa ggccagtcga
     6421 cccggccctc ctggatgaac tccaaaaagt tttcaccctg gaaatggcct acacgatata
     6481 cgtgcctttt tcatgccttt tgggtgatat aatcagaaaa attatcccta atcatgaact
     6541 cgttggagaa cttgctgccc tgtatcttga aagcatttca cccagttcaa ggaatccagc
     6601 aagtgttgaa ccgacaatgc ctggtacggg acccgagtgg gatcctcatg gtggtggctg
     6661 cccgcaagac gacgggcatt ctggtacatt cggtagcgtc cttgtcggca atcgcattca
     6721 gatacctaat gactcccgcc ccgagaatcc tggtcctttg ggaccgatat caggggtggg
     6781 tgggggagga ctcggaagcg gctccgacga caatgccctc aaacaggaac tgccacgaag
     6841 tgtccacggc ctgtcaggaa actggctggc atactggcaa tatgaaattg gtgttagtca
     6901 gcaagatgcc cacttccatt tccatcaaat ccgacttcaa tccttccctg gacactcagg
     6961 tgcagtcaag tgtgtggcgc ctctttccag cgaagacttt tttctctcag gatcaaaaga
     7021 tcggactgta cgcttgtggc cactctacaa ctatggtgat ggcacttccg aaacagctcc
     7081 taggctggtt tatactcaac accgaaaatc agtatttttc gtaggacagc tcgaagcgcc
     7141 acagcacgtt gtaagctgtg acggggccgt gcacgtatgg gatcctttca ccggtaagac
     7201 cctgcgcact gttgagcctc tcgactctag ggtacccttg actgccgtcg cagttatgcc
     7261 ggccccacat acgtctatca ccatggctag cagcgactct acgttgcggt ttgtagactg
     7321 tcggaagccc ggtctgcagc acgaattcag attgggaggt ggacttaacc ccgggctggt
     7381 cagagctttg gctatctctc catcaggccg ctcagtagtg gccggatttt ctagtgggtt
     7441 catggtcctg ctcgacacgc ggaccggtct ggttcttcgg ggctggcccg cgcatgaagg
     7501 tgacattttg cagatcaaag ccgttgaagg aagtgtgctg gtatcatcat ccagcgatca
     7561 cagtctcacg gtgtggaaag agctggaaca gaaaccgaca catcattaca aatcagcctc
     7621 agatccgatc cataccttcg atttgtacgg gagtgaagtt gtgacgggca cggtttccaa
     7681 caagatcggc gtttgtagtt tgctcgaacc accctcacag gccacgacca agctcagtag
     7741 tgagaatttt cgaggaaccc tcacaagtct tgccctgctc ccgacaaaac gccatctgct
     7801 cttgggatca gataatggcg ttattaggtt gcttgcatga tgatctagat ttgggcccaa
     7861 ttcctgcagg attttgcggc cgcttgctga gttggctgct gccaccgctg agcaataact
     7921 agcataaccc cttggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     7981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     8041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     8101 atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa
     8161 tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     8221 ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc
     8281 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     8341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     8401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct tctgacacaa
     8701 cagtctcgaa cttaaggcta gagccaccat gattgaacaa gatggattgc acgcaggttc
     8761 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     8821 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     8881 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     8941 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     9001 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     9061 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     9121 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     9181 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     9241 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     9301 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     9361 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     9421 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     9481 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     9541 gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt
     9601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcctctt
     9661 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     9721 ccgtttctgc ggactggctt tctacgtaat ggtttcttag acgtcaggtg gcacttttcg
     9781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10081 acgttttcca atgatgagca ctttcaaagt tctgctatgt ggcgcggtat tatcccgtat
    10141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa ctatcggagg
    10321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    10381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    10441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    10501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    10561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    10621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    10681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    10741 gattaagcat tggtaattcg aaatgaccga ccaagcgacg cccaaccggt atcagctcac
    10801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11461 gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt
    11521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11581 ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg aacgcacgct
    11641 ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg
    11701 ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa aatttatcag
    11761 gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga
    11821 tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tccacttact
    11881 gtctgccctc gtagccatcg agataaaccg cagtactccg gccacgatgc gtccggcgta
    11941 gaggatcgag atct
//
