LOCUS       sequence-423331-       11966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR81. C-terminal 
            HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTC-HaloTag_WDR81:1-1941
FEATURES             Location/Qualifiers
     source          1..11966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6920..6940
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             6956..7840
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(7912..7930)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7967..8088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7978..7997)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8032..8051
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8333..8690
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8333..8524
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8356..8376)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8541..8676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8741..9535
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8795..8814)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9405..9424
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9599..9647
                     /note="synthetic polyadenylation signal"
     promoter        9803..9907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9908..9976,9977..10768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10126..10145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10926..11514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11006..11025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11630..11913
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcac
     1081 catggcacag ggctctgggg gtcgagaagg tgcactccgg acgcctgctg gtggatggca
     1141 cagcccgcct tcccctgaca tgcaggaatt gcttcgctcc gtagaaaggg atctctctat
     1201 agatccacgc cagcttgcgc cggcacctgg aggtacgcat gtcgtagccc tggtaccggc
     1261 caggtggctc gccagtctga gagatagacg actcccattg ggaccttgcc ccagggctga
     1321 gggacttggt gaggcggagg tgcgaacctt gctgcagagg tctgtccaga ggcttcctgc
     1381 gggttggaca agggtagagg tgcacggtct ccgaaaacgg cggttgtcct atccgctggg
     1441 tggtggcctc ccttttgagg acggaagttg cggccctgaa actctcaccc ggtttatgca
     1501 agaagtagcc gcacaaaact accgaaatct gtggaggcat gcttatcata cgtacgggca
     1561 accgtattcc cattcccctg cgccgagcgc agtacccgca ctggatagtg tgcgacaagc
     1621 tcttcagcgg gtttatggtt gttctttttt gcccgttggc gaaacgacgc aatgcccctc
     1681 ttacgctcga gagggacctt gtcctccacg agggagccct gcctgtcctt ccttgttgcg
     1741 ggcagaggct ctcctcgaga gtccggagat gctgtatgtt gtgcaccctt acgtgcagtt
     1801 tagcctgcat gatgtggtga ccttcagccc agcaaagttg actaatagtc aggcaaaagt
     1861 gctctttatc ttgtttcgag tcctgcgagc catggacgct tgccaccggc aaggtctcgc
     1921 ctgcggtgca ttgtctcttt accatattgc cgtcgatgag aagctctgca gtgagcttag
     1981 attggatctg agtgcttacg aacggcctga ggaggacgag aatgaggagg ccccagtggc
     2041 gcgagacgaa gcgggtatcg tatcccaaga ggagcagggt ggccaacccg gtcaacccac
     2101 gggtcaagaa gagcttagat ctctcgtgct tgactgggtc cacggcagaa tctccaactt
     2161 ccactatttg atgcaactca acagactggc ggggcggagg cagggtgacc cgaattacca
     2221 tccagtcctg ccgtgggtgg tagatttcac aaccccacat ggtcggttcc gagacctccg
     2281 gaaatctaag ttcaggttga acaagggcga taagcaactg gactttacct atgaaatgac
     2341 tcggcaggcc tttgtagcgg gcggagcagg ggggggagag cctccccacg tgccgcatca
     2401 tatctcagat gtcctgtccg acatcaccta ctatgtgtac aaagccagaa ggactcctcg
     2461 gtctgttttg tgtggacacg ttcgcgccca atgggaaccc catgaatatc cagcgtcaat
     2521 ggagcgaatg cagaattgga cgccagacga atgtattcct gagttctaca ccgacccgag
     2581 tatatttcga tctattcacc ccgatatgcc tgacttggat gtgcccgcat ggtgctcaag
     2641 tagccaggag ttcgtagctg cccatcgcgc cttgttggaa tcacgggaag taagccggga
     2701 tctgcaccat tggattgacc ttacattcgg atacaaattg cagggtaaag aggcagttaa
     2761 ggaaaagaac gtttgtcttc atctggttga cgcacacacg catcttgcgt cctacggggt
     2821 tgtccaattg ttcgatcaac cccatccgca aaggctcgct ggcgccccag ctcttgcccc
     2881 agaaccgccg cttataccta aacttctggt gcagacgata caagaaacca ctggtcgaga
     2941 ggattttacc gaaaatcctg ggcaattgcc caacggagtc ggcagaccag tattggaagc
     3001 aaccccctgt gaagcttctt ggactaggga tagacctgtg gccggtgaag atgacctgga
     3061 gcaagccacc gaagcactgg atagtataag cctcgcggga aaggccgggg accagctcgg
     3121 aagcagctct caagccagtc ctggcctcct cagcttttca gtcgcgagtg cctcaagacc
     3181 tgggcggcgc aacaaagccg ccggtgccga cccaggcgaa ggggaggaag ggaggatatt
     3241 gcttccagaa gggttcaatc ctatgcaggc cttggaggag ctggaaaaaa caggaaactt
     3301 ccttgctaag ggccttggcg gcctgctgga ggtcccagag cagcctaggg tacagcccgc
     3361 ggtacccttg cagtgcctgt tgcaccgaga catgcaggcc ttgggtgtcc tcctcgcgga
     3421 aatggtgttt gcgacgcgag tccgaacgct ccaacctgac gctcctttgt gggtgcgctt
     3481 tcaggcagtg cgcggacttt gtacacggca cccgaaggag gtgccagttt cccttcagcc
     3541 tgtattggat acattgttgc agatgtccgg accggaggtt cccatgggtg ccgagcgggg
     3601 taagcttgac caattgttcg agtatcgacc ggtgagtcaa ggacttccac ccccatgccc
     3661 atcacagctg ctgtccccct ttagctcagt agttcccttc cctccttatt tcccggcact
     3721 gcataggttt attctgcttt atcaggctcg acgcgtcgag gatgaggccc aaggccggga
     3781 gttggtattc gccctctggc aacagctggg cgctgtgctt aaagacatta ccccagaggg
     3841 acttgagata ttgctgccct ttgtcctcag ccttatgtcc gaggaacata ccgcggtgta
     3901 tacagcctgg tacctctttg agcctgttgc gaaggctctg ggacctaaga acgcaaataa
     3961 gtacctcctg aaacccctca taggagctta tgagtcccct tgccaattgc acgggaggtt
     4021 ttatttgtat acagattgtt ttgtggccca gctcatggta agattgggcc tgcaggcctt
     4081 tttgacgcac ctgttgccgc acgtactcca agtattggcc ggagctgaag catcacagga
     4141 agagtcaaag gatctggcgg gagctgctga agaagaagag tccggtcttc caggggcggg
     4201 cccaggcagt tgcgcatttg gagaggaaat cccgatggat ggcgaacctc cagcgtcttc
     4261 aggtctcgga ctcccagact atacaagcgg ggttagcttc cacgatcagg ctgacctccc
     4321 cgagaccgaa gatttccaag ctggtctgta tgtaaccgaa agtccacaac cgcaagaagc
     4381 ggaggcagtg tccttgggtc gactcagcga caaatcttct acatccgaaa cttctctggg
     4441 cgaggagagg gcaccggatg aaggaggggc ccctgttgac aaaagctcac ttaggtccgg
     4501 agatagtagt caagatctca aacagtctga aggatccgaa gaagaagagg aggaagagga
     4561 ttcctgtgtt gtgctggagg aggaagaggg agaacaggag gaagttaccg gcgcaagtga
     4621 actgacactt agtgatacgg tgctctcaat ggagaccgta gtcgccggtg gctcaggagg
     4681 agacggggaa gaggaagagg aggccctgcc agaacaatca gaaggtaaag aacaaaagat
     4741 cctccttgac accgcctgta agatggtccg ctggctctcc gctaagttgg gaccaacggt
     4801 tgcgagtcgg cacgtggcgc ggaatctcct gagactgctc acaagctgct acgttggccc
     4861 tactaggcaa cagttcacag tctcctctgg agaatccccc ccactctccg ctggaaacat
     4921 ctaccagaag agaccagtac tgggtgacat cgtctctggg ccagtgttga gctgcttgtt
     4981 gcacatcgca cgattgtatg gcgaaccggt acttacgtat caatacctgc catatatttc
     5041 ttacctcgtg gcccccggct cagcttctgg gccctcccgc ctcaatagcc gaaaagaagc
     5101 gggtctgctt gctgctgtca cgttgactca aaaaattatt gtatacctta gcgacacgac
     5161 actgatggac atcttgccaa gaatcagcca tgaggtgctg cttccagtct tgtcattttt
     5221 gaccagtctt gttacggggt ttccgtccgg tgcccaagca aggactatcc tttgtgtaaa
     5281 aacaatatca ttgatagcac tcatttgcct taggattgga caggagatgg tacaacaaca
     5341 tttgtccgaa cccgtagcta cttttttcca agtctttagt cagctgcacg agctccgcca
     5401 gcaggacttg aagttggatc ctgctggtag gggagagggc caactgcccc aggtcgtttt
     5461 cagcgatggg caacaaaggc cagtcgaccc ggccctcctg gatgaactcc aaaaagtttt
     5521 caccctggaa atggcctaca cgatatacgt gcctttttca tgccttttgg gtgatataat
     5581 cagaaaaatt atccctaatc atgaactcgt tggagaactt gctgccctgt atcttgaaag
     5641 catttcaccc agttcaagga atccagcaag tgttgaaccg acaatgcctg gtacgggacc
     5701 cgagtgggat cctcatggtg gtggctgccc gcaagacgac gggcattctg gtacattcgg
     5761 tagcgtcctt gtcggcaatc gcattcagat acctaatgac tcccgccccg agaatcctgg
     5821 tcctttggga ccgatatcag gggtgggtgg gggaggactc ggaagcggct ccgacgacaa
     5881 tgccctcaaa caggaactgc cacgaagtgt ccacggcctg tcaggaaact ggctggcata
     5941 ctggcaatat gaaattggtg ttagtcagca agatgcccac ttccatttcc atcaaatccg
     6001 acttcaatcc ttccctggac actcaggtgc agtcaagtgt gtggcgcctc tttccagcga
     6061 agactttttt ctctcaggat caaaagatcg gactgtacgc ttgtggccac tctacaacta
     6121 tggtgatggc acttccgaaa cagctcctag gctggtttat actcaacacc gaaaatcagt
     6181 atttttcgta ggacagctcg aagcgccaca gcacgttgta agctgtgacg gggccgtgca
     6241 cgtatgggat cctttcaccg gtaagaccct gcgcactgtt gagcctctcg actctagggt
     6301 acccttgact gccgtcgcag ttatgccggc cccacatacg tctatcacca tggctagcag
     6361 cgactctacg ttgcggtttg tagactgtcg gaagcccggt ctgcagcacg aattcagatt
     6421 gggaggtgga cttaaccccg ggctggtcag agctttggct atctctccat caggccgctc
     6481 agtagtggcc ggattttcta gtgggttcat ggtcctgctc gacacgcgga ccggtctggt
     6541 tcttcggggc tggcccgcgc atgaaggtga cattttgcag atcaaagccg ttgaaggaag
     6601 tgtgctggta tcatcatcca gcgatcacag tctcacggtg tggaaagagc tggaacagaa
     6661 accgacacat cattacaaat cagcctcaga tccgatccat accttcgatt tgtacgggag
     6721 tgaagttgtg acgggcacgg tttccaacaa gatcggcgtt tgtagtttgc tcgaaccacc
     6781 ctcacaggcc acgaccaagc tcagtagtga gaattttcga ggaaccctca caagtcttgc
     6841 cctgctcccg acaaaacgcc atctgctctt gggatcagat aatggcgtta ttaggttgct
     6901 tgcactcgag ccaaccactg aggatctgta ctttcagagc gataacgatg gatccgaaat
     6961 cggtactggc tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta
     7021 cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc
     7081 ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc
     7141 agacctgatc ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca
     7201 cgtccgcttc atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat
     7261 tcacgactgg ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa
     7321 aggtattgca tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt
     7381 tgcccgcgag accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga
     7441 tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt
     7501 cgagatggac cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg
     7561 cttcccaaac gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga
     7621 atacatggac tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg
     7681 cgttctgatc ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc
     7741 tgtggacatc ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag
     7801 cgagatcgcg cgctggctgt ctactctgga gatttccggt taatagaatt ggcatgcaag
     7861 ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     7921 aataactagc ataacccctt ggggcggccg cttcgagcag acatgataag atacattgat
     7981 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     8041 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     8101 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt aagcaagtaa
     8161 aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta caatttcctg
     8221 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg
     8281 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
     8341 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     8401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8461 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8521 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8581 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8641 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt
     8701 cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt
     8761 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     8821 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     8881 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     8941 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     9001 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     9061 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     9121 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     9181 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     9241 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     9301 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     9361 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     9421 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     9481 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     9541 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa
     9601 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat
     9661 aaggatcctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     9721 ccggggtcag caccgtttct gcggactggc tttctacgta atggtttctt agacgtcagg
     9781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    10021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    10081 tcgccccgaa gaacgttttc caatgatgag cactttcaaa gttctgctat gtggcgcggt
    10141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    10201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    10261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    10321 aactatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    10381 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    10441 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    10501 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    10561 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    10621 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10681 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10741 aggtgcctca ctgattaagc attggtaatt cgaaatgacc gaccaagcga cgcccaaccg
    10801 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    11041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    11101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11341 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    11401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat
    11521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaaataca
    11641 ggaacgcacg ctggatggcc cttcgctggg atggtgaaac catgaaaaat ggcagcttca
    11701 gtggattaag tgggggtaat gtggcctgta ccctctggtt gcataggtat tcatacggtt
    11761 aaaatttatc aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat ttttacctgt
    11821 ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg gattatggta
    11881 aatccactta ctgtctgccc tcgtagccat cgagataaac cgcagtactc cggccacgat
    11941 gcgtccggcg tagaggatcg agatct
//
