LOCUS       sequence-423333-       11781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR81. C-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 11781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_WDR81:1-1941
FEATURES             Location/Qualifiers
     source          1..11781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1079..1088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6956..7468
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(7544..7562)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7599..7720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7610..7629)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7664..7683
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7965..8322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7965..8156
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7988..8008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8173..8308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8353..9390
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             9369..9377
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    9414..9462
                     /note="synthetic polyadenylation signal"
     promoter        9618..9722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9723..9791,9792..10583)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9941..9960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10741..11329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10821..10840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11445..11728
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcgc
     1081 caccatggca cagggctctg ggggtcgaga aggtgcactc cggacgcctg ctggtggatg
     1141 gcacagcccg ccttcccctg acatgcagga attgcttcgc tccgtagaaa gggatctctc
     1201 tatagatcca cgccagcttg cgccggcacc tggaggtacg catgtcgtag ccctggtacc
     1261 ggccaggtgg ctcgccagtc tgagagatag acgactccca ttgggacctt gccccagggc
     1321 tgagggactt ggtgaggcgg aggtgcgaac cttgctgcag aggtctgtcc agaggcttcc
     1381 tgcgggttgg acaagggtag aggtgcacgg tctccgaaaa cggcggttgt cctatccgct
     1441 gggtggtggc ctcccttttg aggacggaag ttgcggccct gaaactctca cccggtttat
     1501 gcaagaagta gccgcacaaa actaccgaaa tctgtggagg catgcttatc atacgtacgg
     1561 gcaaccgtat tcccattccc ctgcgccgag cgcagtaccc gcactggata gtgtgcgaca
     1621 agctcttcag cgggtttatg gttgttcttt tttgcccgtt ggcgaaacga cgcaatgccc
     1681 ctcttacgct cgagagggac cttgtcctcc acgagggagc cctgcctgtc cttccttgtt
     1741 gcgggcagag gctctcctcg agagtccgga gatgctgtat gttgtgcacc cttacgtgca
     1801 gtttagcctg catgatgtgg tgaccttcag cccagcaaag ttgactaata gtcaggcaaa
     1861 agtgctcttt atcttgtttc gagtcctgcg agccatggac gcttgccacc ggcaaggtct
     1921 cgcctgcggt gcattgtctc tttaccatat tgccgtcgat gagaagctct gcagtgagct
     1981 tagattggat ctgagtgctt acgaacggcc tgaggaggac gagaatgagg aggccccagt
     2041 ggcgcgagac gaagcgggta tcgtatccca agaggagcag ggtggccaac ccggtcaacc
     2101 cacgggtcaa gaagagctta gatctctcgt gcttgactgg gtccacggca gaatctccaa
     2161 cttccactat ttgatgcaac tcaacagact ggcggggcgg aggcagggtg acccgaatta
     2221 ccatccagtc ctgccgtggg tggtagattt cacaacccca catggtcggt tccgagacct
     2281 ccggaaatct aagttcaggt tgaacaaggg cgataagcaa ctggacttta cctatgaaat
     2341 gactcggcag gcctttgtag cgggcggagc agggggggga gagcctcccc acgtgccgca
     2401 tcatatctca gatgtcctgt ccgacatcac ctactatgtg tacaaagcca gaaggactcc
     2461 tcggtctgtt ttgtgtggac acgttcgcgc ccaatgggaa ccccatgaat atccagcgtc
     2521 aatggagcga atgcagaatt ggacgccaga cgaatgtatt cctgagttct acaccgaccc
     2581 gagtatattt cgatctattc accccgatat gcctgacttg gatgtgcccg catggtgctc
     2641 aagtagccag gagttcgtag ctgcccatcg cgccttgttg gaatcacggg aagtaagccg
     2701 ggatctgcac cattggattg accttacatt cggatacaaa ttgcagggta aagaggcagt
     2761 taaggaaaag aacgtttgtc ttcatctggt tgacgcacac acgcatcttg cgtcctacgg
     2821 ggttgtccaa ttgttcgatc aaccccatcc gcaaaggctc gctggcgccc cagctcttgc
     2881 cccagaaccg ccgcttatac ctaaacttct ggtgcagacg atacaagaaa ccactggtcg
     2941 agaggatttt accgaaaatc ctgggcaatt gcccaacgga gtcggcagac cagtattgga
     3001 agcaaccccc tgtgaagctt cttggactag ggatagacct gtggccggtg aagatgacct
     3061 ggagcaagcc accgaagcac tggatagtat aagcctcgcg ggaaaggccg gggaccagct
     3121 cggaagcagc tctcaagcca gtcctggcct cctcagcttt tcagtcgcga gtgcctcaag
     3181 acctgggcgg cgcaacaaag ccgccggtgc cgacccaggc gaaggggagg aagggaggat
     3241 attgcttcca gaagggttca atcctatgca ggccttggag gagctggaaa aaacaggaaa
     3301 cttccttgct aagggccttg gcggcctgct ggaggtccca gagcagccta gggtacagcc
     3361 cgcggtaccc ttgcagtgcc tgttgcaccg agacatgcag gccttgggtg tcctcctcgc
     3421 ggaaatggtg tttgcgacgc gagtccgaac gctccaacct gacgctcctt tgtgggtgcg
     3481 ctttcaggca gtgcgcggac tttgtacacg gcacccgaag gaggtgccag tttcccttca
     3541 gcctgtattg gatacattgt tgcagatgtc cggaccggag gttcccatgg gtgccgagcg
     3601 gggtaagctt gaccaattgt tcgagtatcg accggtgagt caaggacttc cacccccatg
     3661 cccatcacag ctgctgtccc cctttagctc agtagttccc ttccctcctt atttcccggc
     3721 actgcatagg tttattctgc tttatcaggc tcgacgcgtc gaggatgagg cccaaggccg
     3781 ggagttggta ttcgccctct ggcaacagct gggcgctgtg cttaaagaca ttaccccaga
     3841 gggacttgag atattgctgc cctttgtcct cagccttatg tccgaggaac ataccgcggt
     3901 gtatacagcc tggtacctct ttgagcctgt tgcgaaggct ctgggaccta agaacgcaaa
     3961 taagtacctc ctgaaacccc tcataggagc ttatgagtcc ccttgccaat tgcacgggag
     4021 gttttatttg tatacagatt gttttgtggc ccagctcatg gtaagattgg gcctgcaggc
     4081 ctttttgacg cacctgttgc cgcacgtact ccaagtattg gccggagctg aagcatcaca
     4141 ggaagagtca aaggatctgg cgggagctgc tgaagaagaa gagtccggtc ttccaggggc
     4201 gggcccaggc agttgcgcat ttggagagga aatcccgatg gatggcgaac ctccagcgtc
     4261 ttcaggtctc ggactcccag actatacaag cggggttagc ttccacgatc aggctgacct
     4321 ccccgagacc gaagatttcc aagctggtct gtatgtaacc gaaagtccac aaccgcaaga
     4381 agcggaggca gtgtccttgg gtcgactcag cgacaaatct tctacatccg aaacttctct
     4441 gggcgaggag agggcaccgg atgaaggagg ggcccctgtt gacaaaagct cacttaggtc
     4501 cggagatagt agtcaagatc tcaaacagtc tgaaggatcc gaagaagaag aggaggaaga
     4561 ggattcctgt gttgtgctgg aggaggaaga gggagaacag gaggaagtta ccggcgcaag
     4621 tgaactgaca cttagtgata cggtgctctc aatggagacc gtagtcgccg gtggctcagg
     4681 aggagacggg gaagaggaag aggaggccct gccagaacaa tcagaaggta aagaacaaaa
     4741 gatcctcctt gacaccgcct gtaagatggt ccgctggctc tccgctaagt tgggaccaac
     4801 ggttgcgagt cggcacgtgg cgcggaatct cctgagactg ctcacaagct gctacgttgg
     4861 ccctactagg caacagttca cagtctcctc tggagaatcc cccccactct ccgctggaaa
     4921 catctaccag aagagaccag tactgggtga catcgtctct gggccagtgt tgagctgctt
     4981 gttgcacatc gcacgattgt atggcgaacc ggtacttacg tatcaatacc tgccatatat
     5041 ttcttacctc gtggcccccg gctcagcttc tgggccctcc cgcctcaata gccgaaaaga
     5101 agcgggtctg cttgctgctg tcacgttgac tcaaaaaatt attgtatacc ttagcgacac
     5161 gacactgatg gacatcttgc caagaatcag ccatgaggtg ctgcttccag tcttgtcatt
     5221 tttgaccagt cttgttacgg ggtttccgtc cggtgcccaa gcaaggacta tcctttgtgt
     5281 aaaaacaata tcattgatag cactcatttg ccttaggatt ggacaggaga tggtacaaca
     5341 acatttgtcc gaacccgtag ctactttttt ccaagtcttt agtcagctgc acgagctccg
     5401 ccagcaggac ttgaagttgg atcctgctgg taggggagag ggccaactgc cccaggtcgt
     5461 tttcagcgat gggcaacaaa ggccagtcga cccggccctc ctggatgaac tccaaaaagt
     5521 tttcaccctg gaaatggcct acacgatata cgtgcctttt tcatgccttt tgggtgatat
     5581 aatcagaaaa attatcccta atcatgaact cgttggagaa cttgctgccc tgtatcttga
     5641 aagcatttca cccagttcaa ggaatccagc aagtgttgaa ccgacaatgc ctggtacggg
     5701 acccgagtgg gatcctcatg gtggtggctg cccgcaagac gacgggcatt ctggtacatt
     5761 cggtagcgtc cttgtcggca atcgcattca gatacctaat gactcccgcc ccgagaatcc
     5821 tggtcctttg ggaccgatat caggggtggg tgggggagga ctcggaagcg gctccgacga
     5881 caatgccctc aaacaggaac tgccacgaag tgtccacggc ctgtcaggaa actggctggc
     5941 atactggcaa tatgaaattg gtgttagtca gcaagatgcc cacttccatt tccatcaaat
     6001 ccgacttcaa tccttccctg gacactcagg tgcagtcaag tgtgtggcgc ctctttccag
     6061 cgaagacttt tttctctcag gatcaaaaga tcggactgta cgcttgtggc cactctacaa
     6121 ctatggtgat ggcacttccg aaacagctcc taggctggtt tatactcaac accgaaaatc
     6181 agtatttttc gtaggacagc tcgaagcgcc acagcacgtt gtaagctgtg acggggccgt
     6241 gcacgtatgg gatcctttca ccggtaagac cctgcgcact gttgagcctc tcgactctag
     6301 ggtacccttg actgccgtcg cagttatgcc ggccccacat acgtctatca ccatggctag
     6361 cagcgactct acgttgcggt ttgtagactg tcggaagccc ggtctgcagc acgaattcag
     6421 attgggaggt ggacttaacc ccgggctggt cagagctttg gctatctctc catcaggccg
     6481 ctcagtagtg gccggatttt ctagtgggtt catggtcctg ctcgacacgc ggaccggtct
     6541 ggttcttcgg ggctggcccg cgcatgaagg tgacattttg cagatcaaag ccgttgaagg
     6601 aagtgtgctg gtatcatcat ccagcgatca cagtctcacg gtgtggaaag agctggaaca
     6661 gaaaccgaca catcattaca aatcagcctc agatccgatc cataccttcg atttgtacgg
     6721 gagtgaagtt gtgacgggca cggtttccaa caagatcggc gtttgtagtt tgctcgaacc
     6781 accctcacag gccacgacca agctcagtag tgagaatttt cgaggaaccc tcacaagtct
     6841 tgccctgctc ccgacaaaac gccatctgct cttgggatca gataatggcg ttattaggtt
     6901 gcttgcatct agatttgggc ccaattcctg caggcgagct ctcggctcga gcggcgtctt
     6961 cacactcgaa gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt
     7021 ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat
     7081 ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc
     7141 gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta
     7201 ccctgtggat gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg
     7261 ggttacgccg aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga
     7321 cggcaaaaag atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg
     7381 cctgatcaac cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg
     7441 gcggctgtgc gaacgcattc tggcgtaagg ccgcgactct agggcatgca agctgatccg
     7501 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     7561 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     7621 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     7681 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     7741 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta
     7801 caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     7861 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca
     7921 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     7981 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     8041 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8101 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8161 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8221 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8281 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcga ttcttctgac
     8341 actagcgcca ccatgaagaa gcccgaactc accgctacca gcgttgaaaa atttctcatc
     8401 gagaagttcg acagtgtgag cgacctgatg cagttgtcgg agggcgaaga gagccgagcc
     8461 ttcagcttcg atgtcggcgg acgcggctat gtactgcggg tgaatagctg cgctgatggc
     8521 ttctacaaag accgctacgt gtaccgccac ttcgccagcg ctgcactacc catccccgaa
     8581 gtgttggaca tcggcgagtt cagcgagagc ctgacatact gcatcagtag acgcgcccaa
     8641 ggcgttactc tccaagacct ccccgaaaca gagctgcctg ctgtgttaca gcctgtcgcc
     8701 gaagctatgg atgctattgc cgccgccgac ctcagtcaaa ccagcggctt cggcccattc
     8761 gggccccaag gcatcggcca gtacacaacc tggcgggatt tcatttgcgc cattgctgat
     8821 ccccatgtct accactggca gaccgtgatg gacgacaccg tgtccgccag cgtagctcaa
     8881 gccctggacg aactgatgct gtgggccgaa gactgtcccg aggtgcgcca cctcgtccat
     8941 gccgacttcg gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc cgtaatcgac
     9001 tggtccgaag ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt cttctggcgg
     9061 ccctggctgg cttgcatgga gcagcagact cgctacttcg agcgccggca tcccgagctg
     9121 gccggcagcc ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca gctctaccag
     9181 agcctcgtgg acggcaactt cgacgatgct gcctgggctc aaggccgctg cgatgccatc
     9241 gtccgcagcg gggccggcac cgtcggtcgc acacaaatcg ctcgccggag cgcagccgta
     9301 tggaccgacg gctgcgtcga ggtgctggcc gacagcggca accgccggcc cagtacacga
     9361 ccgcgcgcta aggaggtagg tcgagtttag actctagcca tcacgatggc cgcaataaaa
     9421 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     9481 tcctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     9541 gtcagcaccg tttctgcgga ctggctttct acgtaatggt ttcttagacg tcaggtggca
     9601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9901 ccgaagaacg ttttccaatg atgagcactt tcaaagttct gctatgtggc gcggtattat
     9961 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10021 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10081 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacta
    10141 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10201 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10261 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10321 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10381 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10441 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10501 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10561 cctcactgat taagcattgg taattcgaaa tgaccgacca agcgacgccc aaccggtatc
    10621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    11221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
    11341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11401 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac
    11461 gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga
    11521 ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat
    11581 ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
    11641 gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc
    11701 acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc
    11761 cggcgtagag gatcgagatc t
//
