LOCUS       sequence-423344-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DDB1. N-terminal HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTN-HaloTag_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1067..1957
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1970..1990
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(5503..5521)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    5553..5674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5564..5583)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5618..5637
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5919..6276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5919..6110
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5942..5962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6127..6262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6327..7121
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6381..6400)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6991..7010
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7185..7233
                     /note="synthetic polyadenylation signal"
     promoter        7389..7493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7494..7562,7563..8354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7712..7731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8512..9100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8592..8611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9216..9499
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgcttccga attcatgtcg tacaactacg tggtaacggc
     2041 ccagaagccc accgccgtga acggctgcgt gaccggacac tttacttcgg ccgaagactt
     2101 aaacctgttg attgccaaaa acacgagatt agagatctat gtggtcaccg ccgaggggct
     2161 tcggcccgtc aaagaggtgg gcatgtatgg gaagattgcg gtcatggagc ttttcaggcc
     2221 caagggggag agcaaggacc tgctgtttat cttgacagcg aagtacaatg cctgcatcct
     2281 ggagtataaa cagagtggcg agagcattga catcattacg cgagcccatg gcaatgtcca
     2341 ggaccgcatt ggccgcccct cagagaccgg cattattggc atcattgacc ctgagtgccg
     2401 gatgattggc ctgcgtctct atgatggcct tttcaaggtt attccactag atcgcgataa
     2461 taaagaactc aaggccttca acatccgcct ggaggagctg catgtcattg atgtcaagtt
     2521 cctatatggt tgccaagcac ctactatttg ctttgtctac caggaccctc aggggcggca
     2581 cgtaaaaacc tatgaggtgt ctctccgaga aaaggaattc aataagggcc cttggaaaca
     2641 ggaaaatgtc gaagctgaag cttccatggt gatcgcagtc ccagagccct ttgggggggc
     2701 catcatcatt ggacaggagt caatcaccta tcacaatggt gacaaatacc tggctattgc
     2761 ccctcctatc atcaagcaaa gcacgattgt gtgccacaat cgagtggacc ctaatggctc
     2821 aagatacctg ctgggagaca tggaaggccg gctcttcatg ctgcttttgg agaaggagga
     2881 acagatggat ggcaccgtca ctctcaagga tctccgtgta gaactccttg gagagacctc
     2941 tattgctgag tgcttgacat accttgataa tggtgttgtg tttgtcgggt ctcgcctggg
     3001 tgactcccag cttgtgaagc tcaacgttga cagtaatgaa caaggctcct atgtagtggc
     3061 catggaaacc tttaccaact taggacccat tgtcgatatg tgcgtggtgg acctggagag
     3121 gcaggggcag gggcagctgg tcacttgctc tggggctttc aaggaaggtt ctttgcggat
     3181 catccggaat ggaattggaa tccacgagca tgccagcatt gacttaccag gcatcaaagg
     3241 attatggcca ctgcggtctg accctaatcg tgagactgat gacactttgg tgctctcttt
     3301 tgtgggccag acaagagttc tcatgttaaa tggagaggag gtagaagaaa ccgaactgat
     3361 gggtttcgtg gatgatcagc agactttctt ctgtggcaac gtggctcatc agcagcttat
     3421 ccagatcact tcagcatcgg tgaggttggt ctctcaagaa cccaaagctc tggtcagtga
     3481 atggaaggag cctcaggcca agaacatcag tgtggcctcc tgcaatagca gccaggtggt
     3541 ggtggctgta ggcagggccc tctactatct gcagatccat cctcaggagc tccggcagat
     3601 cagccacaca gagatggaac atgaagtggc ttgcttggac atcaccccat taggagacag
     3661 caatggactg tcccctcttt gtgccattgg cctctggacg gacatctcgg ctcgtatctt
     3721 gaagttgccc tcttttgaac tactgcacaa ggagatgctg ggtggagaga tcattcctcg
     3781 ctccatcctg atgaccacct ttgagagtag ccattacctc ctttgtgcct tgggagatgg
     3841 agcgcttttc tactttgggc tcaacattga gacaggtctg ttgagcgacc gtaagaaggt
     3901 gactttgggc acccagccca ccgtattgag gacttttcgt tctctttcta ccaccaacgt
     3961 ctttgcttgt tctgaccgcc ccactgtcat ctatagcagc aaccacaaat tggtcttctc
     4021 aaatgtcaac ctcaaggaag tgaactacat gtgtcccctc aattcagatg gctatcctga
     4081 cagcctggcg ctggccaaca atagcaccct caccattggc accatcgatg agatccagaa
     4141 gctgcacatt cgcacagttc ccctctatga gtctccaagg aagatctgct accaggaagt
     4201 gtcccagtgt ttcggggtcc tctccagccg cattgaagtc caagacacga gtgggggcac
     4261 gacagccttg aggcccagcg ctagcaccca ggctctgtcc agcagtgtaa gctccagcaa
     4321 gctgttctcc agcagcactg ctcctcatga gacctccttt ggagaagagg tggaggtgca
     4381 caacctactt atcattgacc aacacacctt tgaagtgctt catgcccacc agtttctgca
     4441 gaatgaatat gccctcagtc tggtttcctg caagctgggc aaagacccca acacttactt
     4501 cattgtgggc acagcaatgg tgtatcctga agaggcagag cccaagcagg gtcgcattgt
     4561 ggtctttcag tattcggatg gaaaactaca gactgtggct gaaaaggaag tgaaaggggc
     4621 cgtgtactct atggtggaat ttaacgggaa gctgttagcc agcatcaata gcacggtgcg
     4681 gctctatgag tggacaacag agaaggagct gcgcactgag tgcaaccact acaacaacat
     4741 catggccctc tacctgaaga ccaagggcga cttcatcctg gtgggcgacc ttatgcgctc
     4801 agtgctgctg cttgcctaca agcccatgga aggaaacttt gaagagattg ctcgagactt
     4861 taatcccaac tggatgagtg ctgtggaaat cttggatgat gacaattttc tgggggctga
     4921 aaatgccttt aacttgtttg tgtgtcaaaa ggatagcgct gccaccactg acgaggagcg
     4981 gcagcacctc caggaggttg gtcttttcca cctgggcgag tttgtcaatg tcttttgcca
     5041 cggctctctg gtaatgcaga atctgggtga gacttccacc cccacacaag gctcggtgct
     5101 cttcggcacg gtcaacggca tgatagggct ggtgacctca ctgtcagaga gctggtacaa
     5161 cctcctgctg gacatgcaga atcgactcaa taaagtcatc aaaagtgtgg ggaagatcga
     5221 gcactccttc tggagatcct ttcacaccga gcggaagaca gaaccagcca caggtttcat
     5281 cgacggtgac ttgattgaga gtttcctgga tattagccgc cccaagatgc aggaggtggt
     5341 ggcaaaccta cagtatgacg atggcagcgg tatgaagcga gaggccactg cagacgacct
     5401 catcaaggtt gtggaggagc taactcggat ccattaggat atctagattt gggcccaatt
     5461 cctgcaggat tttgcggccg cttgctgagt tggctgctgc caccgctgag caataactag
     5521 cataacccct tggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     5581 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     5641 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     5701 gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg
     5761 tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     5821 ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct
     5881 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg
     5941 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6001 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6241 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca
     6301 gtctcgaact taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc
     6361 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6421 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6481 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6541 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6601 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6661 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6721 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6781 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6841 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6901 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6961 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7021 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7081 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7141 aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat
     7201 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atcctctttg
     7261 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     7321 gtttctgcgg actggctttc tacgtaatgg tttcttagac gtcaggtggc acttttcggg
     7381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7681 gttttccaat gatgagcact ttcaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaact atcggaggac
     7921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8341 ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc
     8401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8701 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9061 ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc
     9121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9181 atcaaaaagg atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg
     9241 atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg
     9301 ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc
     9361 gcgattgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     9421 gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     9481 ctgccctcgt agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga
     9541 ggatcgagat ct
//
