LOCUS       sequence-423345-        9605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DDB1. C-terminal HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTC-HaloTag_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..9605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4559..4579
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             4595..5479
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(5551..5569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    5606..5727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5617..5636)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5671..5690
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5972..6329
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5972..6163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5995..6015)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6180..6315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6380..7174
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6434..6453)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7044..7063
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7238..7286
                     /note="synthetic polyadenylation signal"
     promoter        7442..7546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7547..7615,7616..8407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7765..7784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8565..9153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8645..8664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9269..9552
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcgc
     1081 caccatgtcg tacaactacg tggtaacggc ccagaagccc accgccgtga acggctgcgt
     1141 gaccggacac tttacttcgg ccgaagactt aaacctgttg attgccaaaa acacgagatt
     1201 agagatctat gtggtcaccg ccgaggggct tcggcccgtc aaagaggtgg gcatgtatgg
     1261 gaagattgcg gtcatggagc ttttcaggcc caagggggag agcaaggacc tgctgtttat
     1321 cttgacagcg aagtacaatg cctgcatcct ggagtataaa cagagtggcg agagcattga
     1381 catcattacg cgagcccatg gcaatgtcca ggaccgcatt ggccgcccct cagagaccgg
     1441 cattattggc atcattgacc ctgagtgccg gatgattggc ctgcgtctct atgatggcct
     1501 tttcaaggtt attccactag atcgcgataa taaagaactc aaggccttca acatccgcct
     1561 ggaggagctg catgtcattg atgtcaagtt cctatatggt tgccaagcac ctactatttg
     1621 ctttgtctac caggaccctc aggggcggca cgtaaaaacc tatgaggtgt ctctccgaga
     1681 aaaggaattc aataagggcc cttggaaaca ggaaaatgtc gaagctgaag cttccatggt
     1741 gatcgcagtc ccagagccct ttgggggggc catcatcatt ggacaggagt caatcaccta
     1801 tcacaatggt gacaaatacc tggctattgc ccctcctatc atcaagcaaa gcacgattgt
     1861 gtgccacaat cgagtggacc ctaatggctc aagatacctg ctgggagaca tggaaggccg
     1921 gctcttcatg ctgcttttgg agaaggagga acagatggat ggcaccgtca ctctcaagga
     1981 tctccgtgta gaactccttg gagagacctc tattgctgag tgcttgacat accttgataa
     2041 tggtgttgtg tttgtcgggt ctcgcctggg tgactcccag cttgtgaagc tcaacgttga
     2101 cagtaatgaa caaggctcct atgtagtggc catggaaacc tttaccaact taggacccat
     2161 tgtcgatatg tgcgtggtgg acctggagag gcaggggcag gggcagctgg tcacttgctc
     2221 tggggctttc aaggaaggtt ctttgcggat catccggaat ggaattggaa tccacgagca
     2281 tgccagcatt gacttaccag gcatcaaagg attatggcca ctgcggtctg accctaatcg
     2341 tgagactgat gacactttgg tgctctcttt tgtgggccag acaagagttc tcatgttaaa
     2401 tggagaggag gtagaagaaa ccgaactgat gggtttcgtg gatgatcagc agactttctt
     2461 ctgtggcaac gtggctcatc agcagcttat ccagatcact tcagcatcgg tgaggttggt
     2521 ctctcaagaa cccaaagctc tggtcagtga atggaaggag cctcaggcca agaacatcag
     2581 tgtggcctcc tgcaatagca gccaggtggt ggtggctgta ggcagggccc tctactatct
     2641 gcagatccat cctcaggagc tccggcagat cagccacaca gagatggaac atgaagtggc
     2701 ttgcttggac atcaccccat taggagacag caatggactg tcccctcttt gtgccattgg
     2761 cctctggacg gacatctcgg ctcgtatctt gaagttgccc tcttttgaac tactgcacaa
     2821 ggagatgctg ggtggagaga tcattcctcg ctccatcctg atgaccacct ttgagagtag
     2881 ccattacctc ctttgtgcct tgggagatgg agcgcttttc tactttgggc tcaacattga
     2941 gacaggtctg ttgagcgacc gtaagaaggt gactttgggc acccagccca ccgtattgag
     3001 gacttttcgt tctctttcta ccaccaacgt ctttgcttgt tctgaccgcc ccactgtcat
     3061 ctatagcagc aaccacaaat tggtcttctc aaatgtcaac ctcaaggaag tgaactacat
     3121 gtgtcccctc aattcagatg gctatcctga cagcctggcg ctggccaaca atagcaccct
     3181 caccattggc accatcgatg agatccagaa gctgcacatt cgcacagttc ccctctatga
     3241 gtctccaagg aagatctgct accaggaagt gtcccagtgt ttcggggtcc tctccagccg
     3301 cattgaagtc caagacacga gtgggggcac gacagccttg aggcccagcg ctagcaccca
     3361 ggctctgtcc agcagtgtaa gctccagcaa gctgttctcc agcagcactg ctcctcatga
     3421 gacctccttt ggagaagagg tggaggtgca caacctactt atcattgacc aacacacctt
     3481 tgaagtgctt catgcccacc agtttctgca gaatgaatat gccctcagtc tggtttcctg
     3541 caagctgggc aaagacccca acacttactt cattgtgggc acagcaatgg tgtatcctga
     3601 agaggcagag cccaagcagg gtcgcattgt ggtctttcag tattcggatg gaaaactaca
     3661 gactgtggct gaaaaggaag tgaaaggggc cgtgtactct atggtggaat ttaacgggaa
     3721 gctgttagcc agcatcaata gcacggtgcg gctctatgag tggacaacag agaaggagct
     3781 gcgcactgag tgcaaccact acaacaacat catggccctc tacctgaaga ccaagggcga
     3841 cttcatcctg gtgggcgacc ttatgcgctc agtgctgctg cttgcctaca agcccatgga
     3901 aggaaacttt gaagagattg ctcgagactt taatcccaac tggatgagtg ctgtggaaat
     3961 cttggatgat gacaattttc tgggggctga aaatgccttt aacttgtttg tgtgtcaaaa
     4021 ggatagcgct gccaccactg acgaggagcg gcagcacctc caggaggttg gtcttttcca
     4081 cctgggcgag tttgtcaatg tcttttgcca cggctctctg gtaatgcaga atctgggtga
     4141 gacttccacc cccacacaag gctcggtgct cttcggcacg gtcaacggca tgatagggct
     4201 ggtgacctca ctgtcagaga gctggtacaa cctcctgctg gacatgcaga atcgactcaa
     4261 taaagtcatc aaaagtgtgg ggaagatcga gcactccttc tggagatcct ttcacaccga
     4321 gcggaagaca gaaccagcca caggtttcat cgacggtgac ttgattgaga gtttcctgga
     4381 tattagccgc cccaagatgc aggaggtggt ggcaaaccta cagtatgacg atggcagcgg
     4441 tatgaagcga gaggccactg cagacgacct catcaaggtt gtggaggagc taactcggat
     4501 ccatcggata tctagatttg ggcccaattc ctgcaggcga gctctcgagc caaccactga
     4561 ggatctgtac tttcagagcg ataacgatgg atccgaaatc ggtactggct ttccattcga
     4621 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga
     4681 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat
     4741 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa
     4801 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt
     4861 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct
     4921 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt
     4981 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc
     5041 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
     5101 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
     5161 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
     5221 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
     5281 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
     5341 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
     5401 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
     5461 tactctggag atttccggtt aatagaattg gcatgcaagc tgatccggct gctaacaaag
     5521 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     5581 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     5641 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5701 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     5761 gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa
     5821 atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     5881 catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa
     5941 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg
     6001 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6061 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6121 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6181 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6241 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6301 gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga
     6361 acttaaggct agagccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6421 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6481 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6541 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6601 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6661 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6721 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6781 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6841 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6901 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6961 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7021 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7081 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7141 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     7201 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt
     7261 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc
     7321 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
     7381 cggactggct ttctacgtaa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7441 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7501 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7561 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7621 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7681 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7741 aatgatgagc actttcaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7801 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7861 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7921 aaccatgagt gataacactg cggccaactt acttctgaca actatcggag gaccgaagga
     7981 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8041 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8101 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8161 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8221 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8281 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8341 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8401 ttggtaattc gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg
     8461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9001 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9121 cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg
     9181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9241 aggatcttca cctagatcct tttatagtcc ggaaatacag gaacgcacgc tggatggccc
     9301 ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag tggattaagt gggggtaatg
     9361 tggcctgtac cctctggttg cataggtatt catacggtta aaatttatca ggcgcgattg
     9421 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga
     9481 acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct
     9541 cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga
     9601 gatct
//
